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Bacillariophyceae diatoms
Chaetoceros calcitrans (5 m) Skeletonema costatum (9 m) Thalassiosira pseudonana (9 m)
Live food aquaculture training course www.aquatrain.org
(CCAP-M, Oban)
Chaetoceros sp.
Sources of algae
CCAP Culture collection of algae and protozoa, Oban, UK. www.ife.ac.uk/ccap/ Algobank Microalgae Strain Bank, Universite de Caen, France. www.unicaen.fr SAG Sammlung von Algenkulturen, Gottingen, Germany www.gwdg.de/~botanik/phykolgia CCMP Provasoli-Guillard National Centre for culture of marine phytoplankton, Westboothbay harbour ME, USA. http://ccmp.bigelow.org UTEX Culture collection of algae at the univesity of Texas at Austin www.bio.utexas.edu/research/utex
Hatchery - Algae requirements Sea bream General assumptions 1) Av. daily algal consumption/m3 larval volume (L) 2) Av daily requirement per 100M rotifer production (L) 3) Algal production volume/m3 daily production (m3) Annual production target (2g juveniles) 20 100 6,7
Requirements
Initial larval rearing vol (m3) Daily larval algae requirement (m3) Daily rotifer production (M) Daily rotifer algae requirement (m3) Total daily algal production (m3) Algal production volume needed (m3) No of 0.5m3 bags needed No of 4m3 tanks required Total algal production volume (m3)
2.000.000
62 1,23 615 0,62 1,8 12 28 0 14
4.000.000
123 2,46 1231 1,23 3,7 25 56 0 28
6.000.000
185 3,69 1846 1,85 5,5 37 54 4 43
10.000.000
308 6,15 3077 3,08 9,2 61 50 12 73
Stock cultures
Approx. 150 - 300 litres per day
Mass cultures
Using algae and yeast there is an average daily requirement of 100 litres of algae for every 100 million rotifers produced. A 6 million hatchery would use 2 x 109 rotifers per day => 2m3 of algae per day. (3 production runs of 2 mill)
Rotifer enrichment.
If all the rotifers were enriched at 2million/ml need 1m3 of tank space and at least 1m3 of algae.
Live food aquaculture training course www.aquatrain.org
Calculating the volume of algae required for green water larval rearing
(6 million juveniles at 20 x 10 cells/ml Nannochloropsis)
6
20 L of algae per m3 of larval rearing per day 6 at 20 x 10 cells/ml A 6 million production hatchery will have approximately 185 m3 of larval rearing. Daily larval rearing requirement of 3.7 m3
High density systems Indoor Outdoor 200 400 150 2,5 20 280 6 40
Shelf lighting