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INFRARED
Spectroscopy
Spectroscopy
What is Infrared?
Infrared radiation lies between the visible and microwave portions of the electromagnetic spectrum. Infrared waves have wavelengths longer than visible and shorter than microwaves, and have frequencies which are lower than visible and higher than microwaves. The Infrared region is divided into: near, mid and far-infrared. * Near-infrared refers to the part of the infrared spectrum that is closest to visible light and far-infrared refers to the part that is closer to the microwave region. * Mid-infrared is the region between these two. The primary source of infrared radiation is thermal radiation. (heat) It is the radiation produced by the motion of atoms and molecules in an object. The higher the temperature, the more the atoms and molecules move and the more infrared radiation they produce. Any object radiates in the infrared. Even an ice cube, emits infrared.
Spectroscopy
Spectroscopy
Infrared Spectroscopy
The bonds between atoms in the molecule stretch and bend, absorbing infrared energy and creating the infrared spectrum.
Symmetric Stretch
Antisymmetric Stretch
Bend
A molecule such as H2O will absorb infrared light when the vibration (stretch or bend) results in a molecular dipole moment change
Spectroscopy
Excited states
h(n1 n0 )
h(n1 - n0)
Infrared absorption occurs among the ground vibrational states, the energy differences, and corresponding spectrum, determined by the specific molecular vibration(s). The infrared absorption is a net energy gain for the molecule and recorded as an energy loss for the analysis beam.
Infrared Spectroscopy
A molecule can be characterized (identified) by its molecular vibrations, based on the absorption and intensity of specific infrared wavelengths.
Spectroscopy
Spectroscopy
Infrared Spectroscopy
For isopropyl alcohol, CH(CH3)2OH, the infrared absorption bands identify the various functional groups of the molecule.
Spectroscopy
Identification and quantitation of organic solid, liquid or gas samples. Analysis of powders, solids, gels, emulsions, pastes, pure liquids and solutions, polymers, pure and mixed gases. Infrared used for research, methods development, quality control and quality assurance applications. Samples range in size from single fibers only 20 microns in length to atmospheric pollution studies involving large areas.
Spectroscopy
Pharmaceutical research Forensic investigations Polymer analysis Lubricant formulation and fuel additives Foods research Quality assurance and control Environmental and water quality analysis methods Biochemical and biomedical research Coatings and surfactants Etc.
Spectroscopy
Dispersion Spectrometer
In order to measure an IR spectrum, the dispersion Spectrometer takes several minutes. Also the detector receives only a few % of the energy of original light source.
Sample
Light source
Fixed CCM
FTIR
In order to measure an IR spectrum, FTIR takes only a few seconds. Moreover, the detector receives up to 50% of the energy of original light source. (much larger than the dispersion spectrometer.)
The interferogram is calculated and transformed into a spectrum using a Fourier Transform (FT).
Spectroscopy
Sample SB
3000
2000 [cm-1]
1000
Spectroscopy
IR Light Source
Intensity Distribution and Temperature Dependency versus Wavelength of Black Body Radiation Energy
105 104 103 102 10 1 10-1 10-2 300K 10-3 200K 0.1 0.2 0.5 1 2 5 10 20 50 100 1000K
6000K
4000K
2000K
500K
10-4
Wavelength l / mm
Spectroscopy
FTIR seminar
Signal strength
Spectroscopy
FTIR seminar
-2l
-l
2l
Fixed mirror
(X)
-2l
l
-l
2l
Spectroscopy
FTIR seminar
(a)
Monochromatic light z
Wavenumber
Time t
(b)
Dichroic light
SAz
I(t)
Time t
b (u)
Wavenumber
Spectroscopy
FTIR seminar
Interferometer interferogram
Single strength
Spectroscopy
Fourier Transform
Fourier transform
SB
4000
Wavenumber[cm-1]
400
(Interferogram)
Wavenumber
D* (l, f) (cmHz1/2W-1)
Spectroscopy
FTIR seminar
Detector Properties
1010
108
4000 Wavenumber[cm-1]
600
Spectroscopy
FT-IR Advantages
Fellgett's (multiplex) Advantage
FT-IR collects all resolution elements with a complete scan of the interferometer. Successive scans of the FTIR instrument are coadded and averaged to enhance the signal-to-noise of the spectrum.
Spectroscopy
Theoretically, an infinitely long scan would average out all the noise in the baseline.
The dispersive instrument collects data one wavelength at a time and collects only a single spectrum. There is no good method for increasing the signal-to-noise of the dispersive spectrum.
FT-IR Advantages
Connes Advantage
an FT-IR uses a HeNe laser as an internal wavelength standard. The infrared wavelengths are calculated using the laser wavelength, itself a very precise and repeatable 'standard'. Wavelength assignment for the FT-IR spectrum is very repeatable and reproducible and data can be compared to digital libraries for identification purposes.
Spectroscopy
FT-IR Advantages
Jacquinot Advantage
FT-IR uses a combination of circular apertures and interferometer travel to define resolution. To improve signal-to-noise, one simply collects more scans. More energy is available for the normal infrared scan and various accessories can be used to solve various sample handling problems. The dispersive instrument uses a rectangular slit to control resolution and cannot increase the signal-tonoise for high resolution scans. Accessory use is limited for a dispersive instrument.
Spectroscopy
Spectroscopy
Spectroscopy
Spectroscopy
Spectroscopy
Apodization
Spectroscopy
Spectroscopy
Spectroscopy
Autoalignment for all models (The interferometer optics can always be aligned by the PC) In addition to proven technology for Rapid scanning and vacuum capabilities; a Step scan capability enables time-resolved studies similar to research models by Nicolet, Bruker and Bio-Rad. IR imaging with IMV-4000 multi-channel microscope for all models (Rapid scanning with a linear array MCT detector ) PC communication and control using USB Aperture of 7.1, 5.0, 3.5, 2.5, 1.8, 1.2, 0.9, 0.5 mm diameter for FT/IR-4100/4200 Spectra Manager II (cross-platform software suite for JASCO spectroscopy systems) (Spectra Manager CFR: 21 CFR Part 11 compliance) Research model capability (Upgradeable wavelength extension, high resolution, step scan) Improved Water Vapor and CO2 Compensation
Spectroscopy
FTIR4000 Series
FT/IR-4100 FT/IR-4200
No additional optics for IR microscope interface Standard apertures for optimum S/N and resolution capability Easy replacement of light source and detector
Microscope
Polymer shell Improved instrument design Compact size Sample compartment with same size as a higher class model
Aperture
FT/IR-400 Plus
Spectroscopy
Spectroscopy
Analog circuit
ADC
Pre-amp.
Pre-amp.
Photo coupler
Photo coupler
Clock
24-bit AD
HeNe laser
Find the zero crossings, then interpolate a matching set of IR data points.
HeNe laser
Over sampling method
Reduction of high frequency noise by over sampling with a 16 times greater number of sampling points enables improvement of the S/N ratio.
Spectroscopy
FTIR6000 Series
- Upgradeability - Wide wavenumber range - Full vacuum capability - Step scan upgrade
FT/IR-600Plus
Spectroscopy