EX.

5: ABO SOLUBLE ANTIGENS

BSMT 3B

GROUP 9
BACANI, RONALINE ANNE L. JOCSON, JANCY R.

OCAMPO, KARL A.
VELASCO, PRINCESS ROCHELLE S.

INTRODUCTION
integral parts of the membranes of RBCs, endothelial cells, platelets,
lymphocytes, and epithelial cells

found in all body secretions presence is dependent on the ABO genes inherited 80% of the random US population

GUIDE QUESTIONS:
Why is there a need to inactivate the saliva?

The saliva must be boil for about 5-10 minutes to destroy the enzymes
so that the saliva must consist mainly of water so an equal saline should be added to ensure that that red cell are not hemolyzed. And in using the saliva you can indicate if a patient is a secretor or nonsecretor through agglutination.

GUIDE QUESTIONS:
Why is there a need to dilute the anti-sera?

It will be necessary to dilute the Anti-A and the Anti-B to a point where
only a one plus reaction is obtained against the appropriate red cells. And anti sera must be diluted so that its antibody titer is more closely matches the antigen level in the saliva.

GUIDE QUESTIONS:
What is the principle of the test?

Certain blood group substances occur in soluble form in a large proportion (78%) of individuals in secretions such as saliva and gastric juice. These individuals are termed secretors (they possess the Se gene) and secrete antihuman globulin (ABH)soluble antigens. These water soluble blood group substances are readily detected in very minute quantities because they have the property of reacting with their corresponding antibodies and thereby neutralizing or inhibiting the capacity of the antibody to agglutinate erythrocytes possessing the corresponding antigen. The reaction is termed hemagglutination inhibition and provides a means of assaying the relative activity or potency of these water-soluble blood group substances.

GUIDE QUESTIONS:

POST-LAB CONFERENCE QUESTIONS:

How are ABH substances formed?

The formation of ABH antigens results from the interaction of genes at

three separate loci. These genes do not actually code for the production of antigens but rather produce specific glycosyltransferase that add sugar to a basic precursor substance, A B H antigens are formed from the same precursor material to which sugar are attached in response to specific enzyme transferases elicited by an inherited gene.

POST-LAB CONFERENCE QUESTIONS:

What is the importance of testing for ABH substances?

To know if the person in secretor or non-secretor

POST-LAB CONFERENCE QUESTIONS:

Are there any other applications of this procedure?

In the past, test for ABH secretion have been used to establish the
true ABO group of an individual whose RBC antigens are poorly developed.

REFENCES:
Harmening, D.M. (2012). Modern Blood Banking & Transfusion Practices 6th Edition.
F.A. Davis. Page 126-127

http://faculty.madisoncollege.edu/mljensen/BloodBank/Lab_Manual/determining_sec
retor_status.htm

http://exa.unne.edu.ar/bioquimica/inmunoclinica/documentos/Biotec_Anti_H_Lectin.
pdf

http://pathmicro.med.sc.edu/mayer/rx-12.jpg http://davisplus.fadavis.com/product/modern-blood-banking-transfusion-practicesharmening