EX. 3: ABO GROUPING (FORWARD) EX.

4: ABO GROUPING (REVERSE)

BSMT 3B
Group 9: Bacani, Ronaline Anne L. Jocson, Jancy R. Ocampo, Karl A. Velasco, Princess Rochelle S.

INTRODUCTION
1900 karl landsteiner Test performed to determine an individual's blood type 4 main blood groups: A, B, O, or AB Important in transfusion medicine In transfusion medicine

EXERCISE 3: ABO GROUPING (FORWARD) GUIDE QUESTIONS:

1. List some advantages of the tube method over the slide method of ABO grouping.

It allows for fairly long incubation Centrifugation Simplicity of reading and grading of results. Clean and more hygienic. Requires smaller volume of reagents.

EXERCISE 3: ABO GROUPING (FORWARD) GUIDE QUESTIONS:

2. Restate the principle of ABO forwarding typing. Antigens present on RBCs are detected by mixing the red cells with specific anti-sera and observing agglutination, either after centrifugation (tube method) or after standing at room temperature (slide method). Agglutination is a positive reaction

EXERCISE 3: ABO GROUPING (FORWARD) POST-LAB CONFERENCE QUESTIONS:

1. List some precautions in ABO grouping and explain briefly.
Expired reagents Proper incubation Equal amount of anti-sera and the blood sample

EXERCISE 3: ABO GROUPING (FORWARD) POST-LAB CONFERENCE QUESTIONS:

2. Why is it necessary to wait for two minutes before reading the result of the slide method?

In slide method, you must wait until two minutes to let the antigen to react to the anti-sera.

EXERCISE 4: ABO GROUPING (REVERSE) GUIDE QUESTIONS:

1. What is the importance of reverse grouping? Identifies the presence of antibodies Confirms the reaction obtained by the forward grouping test Tests the serum/plasma from the recipient or donor with group A red cells and group B red cell

EXERCISE 4: ABO GROUPING (REVERSE) GUIDE QUESTIONS:

2. What are the limitations of the reverse groupings?
False negative reactions False positive reactions

EXERCISE 4: ABO GROUPING (REVERSE) POST-LAB CONFERENCE QUESTIONS:

1. List some precautions in performing the reverse type. Explain each Use the recommended strength of cell suspension Do not warm the ABO reagent before performing the test. If an antibody seems to be missing or weak in the reverse typing, add 2 additional drops of patients serum and centrifuge, if still missing decrease the temperature for 10 minutes and centrifuge Use white background in reading the result

EXERCISE 4: ABO GROUPING (REVERSE) POST-LAB CONFERENCE QUESTIONS:

2. Discuss the difference between antiserum and patient ABO antibodies. Antiserum refers to the blood from an immunized host from which clotting proteins and red blood cells have been removed. An antibody is a protein produced by the body's immune system when it detects harmful substances, called antigens.

REFERENCE
Harmening, D.M. (2012). Modern Blood Banking & Transfusion Practices 6th Edition. F.A. Davis. http://www.austincc.edu/mlt/clin2/abo1.html http://www.nlm.nih.gov/medlineplus/ency/article/002223.htm faculty.madisoncollege.edu www.bloodindex.org biology.clc.uc.edu http://faculty.madisoncollege.edu/mljensen/BloodBank/lectures/abo_blood _group_system.htm http://emedicine.medscape.com/article/1731198-overview