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Objectives
Discuss lab values related to anemia management
>Interpret CBC results, including H/H, RDW (red cell distribution width), MCV (mean corpuscular volume) and MCHC (mean corpuscular hemoglobin concentration) >Examine iron study results, focusing on iron saturation and ferritin
Objectives
Discuss the complex relationship between calcium,
phosphorus, vitamin D and PTH >Review bone and mineral pathophysiology >Discuss why interventions involve thinking about all four of the above lab values >Outline when lab values are only abnormal vs when they are alarming >Discuss treatment of bone and mineral abnormalities with medications and surgery
Objectives
Discuss how dialysis adequacy is determined and used
in a clinical setting >Discuss URR, Kt/V and PET, how to calculate these measures and how to interpret the results >Discuss the advantages and disadvantages of URR (Urea Reduction Ratio) vs Kt/V >Examine when, why and how the dialysis prescription should be adjusted
Plan
Briefly present basic information for each topic
Hopefully, present new information for each topic Case studies will help to enhance understanding of
information presented
Anemia
Anemia is defined as a decrease in red blood cells
(RBCs)
Anemia
Decrease in RBCs can be due to a variety of factors..
>Abnormal destruction of red blood cells (e.g. hemolytic anemia, sickle cell disease) >Lack/decreased cell production from bone marrow (e.g. aplastic anemia, myeloproliferative disorders) >Blood loss (e.g. GI Bleed) >Lack of substances needed to produced RBCs >All of the above seen in people with CKD
RELATED INFORMATION
Stimulates bone marrow to produce RBCs To produce hemoglobin
DNA
Chronic inflammation 2/2 infection and/or autoimmune disorders (e.g. Lupus) impairs DNA synthesis => anemia Reticulocyte count reflects ability of bone marrow to produce RBCs. Retic ulocyte = immature RBCs Anemia in ESRD, can see decreased RBC production (lack of substances to make cells) and increased retic count. A very elevated retic count points to increase RBC destruction or hemolysis as a potential cause of anemia.
Bone marrow
Anemia Management
Current practice
>If Hgb less than 10 g/dl x 2, start Epogen/Aranesp >Hgb levels checked at least monthly (KDOQI) >Goal: maintain Hgb between 10 and 12 g/dl >CREATE and CHOIR study >Do not know optimal Hgb for people with CKD >If Hgb exceeds upper limit (12 g/dl) or increases more than 1 g/dl in 2 wks: >Hold the dose (per FDA) >No benefits to hemoglobin > 13 g/dl. In fact, increases the risk of clots, vascular events (heart attack and stroke) and death
Anemia Management
New?
>TREAT--Trial to Reduce Cardiovascular Events with Aranesp Therapy (people not on dialysis) >Study completed in 2009 >Recommends Hgb >10 and <11 g/dl >Reduce risk of clots, heart attacks, stroke and death >Several other studies currently underway >Will guidelines for people on dialysis also change?
Anemia
Lets define lab values reported in a CBC results:
>Red blood cells >Hemoglobin >Hematocrit >Reticulocyte count >MCV (mean corpuscular volume) >MCHC (mean corpuscular hemoglobin concentration) >RDW (red cell distribution width)
>Carries oxygen
*Ly, et al (200$)
CKD abnormal H/H; what are critical Hgb values? Hgb values below 5 g/dl can cause heart failure
Hemoglobin
Hematocrit
Hematocrit
>% of RBC in plasma (liquid part of the blood) >Increase/decrease of plasma volume affects the hematocrit values >Decrease occurs with over hydration (diluted) >Increase occurs with under hydration (concentrated blood volume) >How would Hct change before/after dialysis? >What lab value used to dose epo? Why? >If RBC and Hgb are normal, estimate Hct by multiplying the Hgb times 3. (10 x 3 = 36)
Reticulocyte Count
Reticulocytes are immature forms of erythrocytes (also called
RBCs) >Up to 1.5% normal in men >Up to 2.5% normal in women >Low retic count seen with folic acid deficiency >High retic count seen when the bone marrow is responding to an increase need for RBCs. Bone marrow cant produce enough mature RBCs fast enough, so it does the next best thing, increases the production of immature RBCs >What would you expect a retic count to be in a person with ESRD on dialysis?
>Indicator of variation in the size of red blood cells >Values > 14.5% = RBC vary a lot in size >Immature red cells usually larger >RDW increased in those with ESRD Why? Bone marrow working hard to produce enough red blood cells but cant produce enough mature cells to keep up with demand.
MCV (mean corpuscular volume) (size of RBC) MCHC (mean corpuscular hemoglobin concentration) (% RBC in fluid)
80-100 femoliters < 80 = Iron deficiency anemia, congenital anemias 32-35% < 32% = Iron deficiency anemia
> 35% = same as 32-35% = above Anemia due to blood loss or a chronic disease
Anemia
Case study
>E.R. is a 39 y/o Hispanic female. Separated with 3 children, ages 12, 13 and 19 years old. >ESRD of unknown etiology; transplant in 2003. Kidney was from her sister. >Rejection (per renal biopsy) August 2010; restarted dialysis in August 2010. >Receives dialysis via ED every 4-5 days. >Receives Aranesp every 2-3 weeks with dialysis.
>What are normal vs. abnormal values? >What information does the abnormal lab values tell you? >Are there any alarming/critical lab values?
CBC Results
Test RBC (red cell count) HGB (hemoglobin) HCT (hematocrit) Retic count (reticulocyte count) Range 4.00-5.20 12-16 g/dl 36-46 % 0.5% - 1.5%/2.5% Patient = E.R. 2.99 million 9.2 g/dl 26.9 % 3.0%
80-100
31-37
90.0-------------75%
34.0%-----------28%
Anemia
Case Study
>32 year old male, T.A., mentally challenge >Lives with his mother >ESRD on hemodialysis 2/2 neurogenic bladder and >Gout >HTN >Sickle cell trait
CBC Results
Test RBC (red cell count) HGB (hemoglobin) HCT (hematocrit) Retic count (reticulocyte count) MCV (mean corpuscular volume) Range 4.00-5.20 12-16 g/dl 36-46 % 0.5% - 1.5%/2.5% 80-100 FL Patient = T.A. 3.52 10.8 g/dl 32.2 % Not reported 91 FL 34 g/dl
16.4%
Measuring Iron
Where iron is found
>Ferritin >Is a protein that binds to iron; helps to transport iron in the body >Most ferritin is found in the liver, spleen, muscle and bone marrow with a small amount found in the blood >Normally, 1 ng of ferritin (in blood) = 10 ng of iron stores (in liver, spleen, muscle and bone marrow) >Ferritin is a proxy measure for iron stores and has it s limitations.
Image retrieved August 11, 2011 from http://www.google.com/imgres?q=diagram+iron+stores+body&um...
Ferritin
Low ferritin levels usually indicates iron deficiency
High ferritin levels, however.. >Does not necessarily indicate adequate iron stores >Many factors can increase ferritin levels, e.g. recent iron infusion, infection,
inflammation, e.g. autoimmune disorders, malignancy, blood transfusions (250 mg of iron/1 unit packed red cells) >Wait two weeks before measuring iron stores after giving iron load (more than 125 mg/week) >Can become iron toxic (ferritin greater than 1000) >High levels can be due to inherited disorders or too much iron administration >Toxic levels of iron can cause organ failure and death
DRIVE STUDY
Dialysis Patients Response to IV iron with elevated ferritin
Study (DRIVE) >Provided some clarification for safe upper limits of ferritin levels in hemodialysis patients. >Ferric gluconate (ferrlecit) administration is superior to no iron therapy in anemic dialysis patients receiving epogen and ferritin levels of 500 to 1200 ng/ml and Tsats of <or=25%.
Iron Saturation
Complete name = transferrin iron saturation or Tsat
>Estimates ability to bind iron and transport it to various sites in the body >Serum iron / total iron binding capacity X 100 More sensitive than ferritin; not affected by inflammation/infection
Iron Deficiency
Many causes
>Blood loss >Celiac disease (decreases absorption of iron) >Hemolysis (RBC breaks apart) >Gastric bypass (decreases absorption) >Epogen administration, etc. Must identify cause of iron deficiency before treating
deficit >? external iron loss >Need more iron Decreasing ferritin and increasing Hgb = iron moving from storage to hemoglobin (e.g. in response to epogen administration) Increasing ferritin and decreasing sats and decreasing Hgb = inflammation >Increase ESA dose
Case Study
Case Study
>F.L. 86 yo female who attends the anemia/CKD clinic. History significant for. >HTN >CKD IV >Anemia >Unable to tolerate po iron supplements due to GI upset
Case Study
TEST/ INTERVENTION Hemoglobin DATE = 11/8/2010 8.8 (decreasing) DATE = 12/22/2010 DATE = 1/17/2011 7.7 9.5
Hematocrit
Saturation Ferritin BUN Creatinine GFR Aranesp dose
28.4
none none 54 (increasing) 2.4 (increasing) 23 ml/min Increased to 200 mcg
25.3
10% 33 55 2.35 24 ml/min 200 mcg
30.9
none none 39 2.04 28 ml/min 200 mcg
>If Hgb is decreasing >Dont miss other causes of anemia >Increase epogen Look at iron sats and ferritin >If iron sats and ferritin both low, give iron >Remember iron is stored in places we dont measure, so look at the entire clinical picture
Vitamin D2-3
Vitamin D comes from
sun, food and our body 25-hydroxyvitamin D2 produced in the liver Normally kidneys produce an enyzme that converts D2 to D3 (1,25 dihydroxyvitamin = calcitriol)
Vitamin D
A complex group of fat-soluble substances (D1-D5)
>D2 = ergocalfciferol >Sources >Foodonly found in seafood, mushrooms, egg yolks and fortified foods >OTC: Generic Vitamin D >Prescription: Drisdoll >Changed in the liver to 25-hydroxycholecalciferol (25-OH) >Measured in those with CKD Stages 3-5 >25-OH changed in normal kidneys to 1,25 dihydroxycholecalciferol >Measured in those with CKD Stage 3-6
Vitamin D3
D3 = cholecalciferol
>D3 = 1,25 dihydroxycholecalciferol >Decreased amounts produced in CKD >Also referred to as active Vitamin D >Sources: Calcitriol (Rocaltrol) Hectoral (doxercalciferol) Zemplar (paricalcitrol) Sunlight (converted to Vitamin D3 in the skin)
Vitamin D
Lab ranges
>25OH = < 30/32 >1,25 = 18-78 >Controversy on what level is normal & too high What does Vitamin D do? >Helps maintain serum calcium and phosphorus levels/regulates release of calcium and phosphorus from the bone >Increases calcium absorption from the intestines >Suppresses PTH synthesis
Calcium
Functions
>Maintains bone structure >Plays a major role in nerve conduction >Assists with muscle contraction/relaxation Most calcium found in bone Serum calcium binds to albumin >Serum calcium = 6.5 Albumin = 2.5 >0.8 x (4.0-2.5) + 6.5 = 7.7 >Corrected serum calcium more accurate >Corrected total calcium 8.4 to 9.5 mg/dl
Hypercalcemia
Long term consequences for those with CKD
>Increased risk CV calcifications (larger arteries) >Calciphylaxis (soft tissue) Serum calcium > 13.0 Causes >Medications (calcium acetate, zemplar) S/S of hypercalcemia >Depression, anxiety, muscle weakness, cognitive dysfunction, fatigue, hypertension, constipation >ECG changes/arrhythmias
Hypocalcemia
Serum calcium < 6.5
>Numbness/tingling in perioral area, fingers, toes >Muscle cramps or tetany (muscle spasm or tremors) >Seizures
http://morningreporttgh.blogspot.com/2010/03/h ypocalcemia.html
community.wegohealth.com
Hypocalcemia
Causes
>CKD (usually CKD Stages 5 & 6) >Medications (e.g. Cinacalcet, Hectoral) >Rapid correction of acidemia (CO2 low) during hemodialysis can trigger tetany and seizures >Hungry Bone Syndrome after parathyroidectomy >Severe decrease in serum calcium due to abrupt decreased in PTH release; change upsets balance of calcium moving to and from the bones
Phosphorus
85% of phosphorus is found in bone and teeth
Has many functions
>Helps maintain health bone and teeth >Essential for storage of energy (ATP) >Helps maintain tissues, cells, DNA, and RNA Phosphate = 3.5 to 5.5 mg/dl
Hyperphosphatemia
Serum levels greater than 12
May be asymptomatic Signs and symptoms, if present
Elevated Phosphorus
What is the role of dietary restriction in decreasing
serum phosphorus levels? >Much phosphorus is found in high quality protein foods >Need high protein intake to prevent muscle wasting but can limit dairy, some vegetables, processed foods and colas >Goal protein intake = 1 gm protein per kg of body weight per day
PHOS*
170 mg 60 mg 265 mg 340 mg 265 mg 25 mg 40 mg 1355 mg
PROTEIN*
12 gms 4 gms 36 gms 2 gms 32 gms 1 gms 0 gms 88 gms
>Two eggs >Two pieces toast Lunch >Grilled chicken-4 oz >Garden salad-2 cups Dinner >Steak-4oz >Green beans-1cup >Apple-medium, fresh
*www.davita.com
>7000 mg X 0.6 = 4200 mg phosphorus/wk 800 mg eliminated/HD treatment = 2400 mg/wk 4200 2400 = 1800 mg Net + phosphorus balance 1800 mg per week >1 Renagel binds about 100 mg phosphorus 1 pill/meal X 100 = 300 x 7 days = 2100 mg/wk 1800 mg 2100 mg = 300 mg negative balance/wk
Hypophosphatemia
Serum levels less than 2.5
May be asymptomatic Causes
>Not eating =>> malnutrition Symptoms, when present >Muscle weakness (e.g. diplopia, dysphagia) >Ventricular arrhythmias >Neuro manifestions (e.g. confusion, coma, seizures) >Poor oxygenation (phosphorus and ATP)
the serum phosphorus to increase Kidney does not reabsorb calcium and vitamin D is not activated, causing decreased serum calcium levels Vitamin D is not activated, causing parathyroid gland hypertrophy and hyperplasia >Decreased serum calcium and increased serum phosphorus levels caused increased secretion of PTH
>Calcitriol (Vitamin D) deficiency >Hyperphosphatemia kidneys no longer excrete phosphate >Decreased Vitamin D and increased phosphorus causes hypocalcemia
Major factors responsible for stimulating PTH are
>Hypocalcemia (sensed by receptors on parathyroid gland => increased secretion of PTH) >Decreased vitamin D levels (1,25 dihydroxyvitamin D = calcitriol) >Hyperphosphatemia
http://www.medscape.com/viewarticle/518757_2
>Optimal PTH levels in advanced kidney disease not known Phosphate = 3.5 to 5.5 mg/dl Corrected total calcium 8.4 to 9.5 mg/dl Calcium-phosphate product < 55 mg2/dl2 >Larger doses Vitamin D analogs associated with increased calcium and phosphorus
Outcomes
Case Study
S.M., an 89 yo with HTN, DM, CVA (residual left sided
weakness), MVR , CABG and CKD V >Very knowledge about dietary content for potassium and phosphorus >Medications >Coreg, Lotrel, Lasix, Mirtazapine, ASA, MVI, Vitamin D 1000 u/day, calcium acetate 667 mg/meal >Pleasant, alert and oriented >BUN 107, Creatinine 7.48, GFR 6 ml/min, K 4.8, CO2 15-----Decision made to start dialysis
phosphorus, vitamin D and PTH-to decide if any actions are needed While can shoot for stated lab values, getting all labs values within stated goal range can be very difficult Must always consider how the person feels/looks when interpreting lab results
Dialysis Adequacy
Used to determine if enough dialysis is being delivered
of normal kidney function/adequacy is relative term >Intermittent dialysis (3x per week) inefficient, only dialyzing out toxins about 7% of the time while the body produces toxins 100% of the time
>Urea = being water soluble, it is easily measured However, there are 90 different compounds that are toxins that we dont measure. Many of these compounds are more toxic than urea. >No current measure of adequacy for these different toxins Conventional dialysis has its limitations: >It removes urea (small, water soluble molecules) >Weekly clearance of urea = about 1/6th of normal physiologic clearance (what would be cleared by healthy kidneys) >28% of toxins are protein bound and not easily removed by dialysis
Yavuz, et al (2005)
Dialysis Adequacy
Adequacy is important since under-dialysis can cause:
>Weakness and fatigue >Weight (muscle) loss > Nausea, decreased appetite > Sleep disturbances
>URR = Urea Reduction Ratio (HD) >Kt/V (HD and PD) >PET = Peritoneal Equilibration Test (PD)
However, people usually live longer and have fewer hospitalizations if URR >= 60% >So, if no one number determines adequate dialysis, how does one choose a URR goal?
Usually measured once per month
Kt/V
Also measures how much urea is removed during dialysis,
but takes into account two additional factors: >Urea made by the body during dialysis >Urea removed during dialysis along with excess fluid Goals* (many different values found in literature) >For CAPD, Kt/V = 2.0 >Kt/V = 1.7 is minimal dose >For HD (adults and peds), Kt/V = 1.2 is minimal dose >As little as 3% residual renal function can increase the Kt/V calculation from 1.2 to 1.65 *KDOQI
Kt/V
SYMBOL K EXPLANATION Rate at which blood passes through the dialyzer in ml/min
Kt
Volume of blood cleared of urea during one dialysis treatment Volume of water in a persons body
Calculating Kt/V*
Example
>Dialyzers clearance = 400 ml/min >Treatment time = 210 min (3.5 hours) >Kt = 400 ml/min x 210 min = 84,000 ml (84 L) >V = volume >Weight = 70 kg. 60% body water (average) >70 kg. x .60 = 42 >Kt/V = 84/42 = 2.0 >Would you/could you make any dialysis changes? *http://kidney.niddk.nih.gov/kudiseases/pubs/hemodialys is dose
>1.2 (goal)/2.0 (achieved) = 0.6 >3.5 - .6 = 2.9 hours (decrease from 3.5 hours)
Increasing the t (time)
>if the Kt/V is 0.9 but the goal is 1.2: 1.2/0.9 = 1.33 or 1.33 times more Kt needed 3 hours (current) x 1.33 = 4 hours
>URR measures urea removed during dialysis >Kt/V adds the amount of urea removed with excess fluid More weight loss during dialysis will yield a higher Kt/V for the same URR URR may be lower than usual if large volume removed Kt/V of 1.2 ~ URR 63%
>Increased the blood flow (Qb) >Increase treatment time >Use a larger dialyzer >Increased dialysis solution flow rate (Qd) >600 ml/min to 800 ml/min >Assess access &/or needle placement for problems
Nocturnal/Home Dialysis
Nocturnal
>3x/week, decreased blood flows, longer treatment times Home Dialysis >5-6 times per week, shorter treatment times Since both treatment modalities lead to feeling better/improved lab results; challenges current concepts of adequacy
membrane area times permeability, so a test is useful to determine the function of the peritoneal membrane >Assesses rate at which solutes (substance dissolved in fluid) equilibrate between the peritoneal capillary blood and dialysate >Solutes = creatinine, urea, phosphate, proteins commonly measured >Dextrose concentration responsible for UF
Diffusion
PET
Standardized 4-hour procedure
>Measures dialysate creatinine and glucose levels at 0, 2 and 4 hours after dialysis solution is infused into the abdomen and serum creatinine and glucose levels at any time during the test. >Performed several weeks after PD initiated and when clinical problems arise, e.g. suspect altered membrane transport
PET
Transport rates assessed by calculating the rates when
equilibrium is reached between the peritoneal blood and dialysate >D/P ratio = solute concentration in dialysate/ solute concentration in plasma (blood) >D/DO = Decrease in dialysate glucose concentration over time Expressed as standard deviation (SD)SD tells you how much variation from the average
Dialysate Creatinine Creat. D/P 0 hour 0.5 0.063 2 hour 4.5 0.563 4 hour 6 0.750
Serum Creatinine @ 2 hrs = 8 Serum Glucose 257
Rapid Transporters
Those with high rates of diffusion/osmosis
>Transport small molecules (e.g. urea, creatinine, glucose) quickly >Leads to equilibration between dialysate and blood early in the dwell >If fluid left in peritoneum, it will be continuously absorbed by the lymphatics, potentially leading to poor UF and volume expansion >Do best with short dwell times >May benefit from icodextrin dialysate solution >Poorly absorbed so osmotic gradient maintained
Decreased Clearance?
Increasing BUN and creatinine could be due to:
>Poor compliance >High protein intake or metabolic acidosis >Decreased peritoneal permeability >Slow transporter >UF continues through out the dwell; clearance continues through out long dwell exchange >Increase inflow dialysate volumes to increase clearance
>Could be due to increased lymph absorption or catheter malfunction If PET shows increased clearance >? Peritonitis >If UF inadequate ? membrane failure If PET shows decreased clearance and decreased UF >? membrane failure
PET
Test can be used to:
>Predict dialysis dose (# cycles and dwell duration) >Help choose peritoneal dialysis regimen >Classify peritoneal dialysis transport (rapid and slow transporters) >Calculate creatinine clearance >CCL = (D/P) X V Peritoneal characteristics change over time >Peritonitisproblems with UF common due to increase glucose absorption that occurs during infection
products. Currently there is no way to measure all the toxins that accumulate in the blood. While Kt/V and URR help us to measure outcomes, they have limitations. In the end, it is as important to look at the entire clinical picture as it is to calculate Kt/V and URR. >How does the person feel? >Is his/her weight stable? PET results can help >Determine dwell times and # cycles needed to clear toxins. >Confirm impending membrane failure.
References
Coyne, D.W., Kapoian, T., Suki, W., Singh, A.K., Moran, J.E., Dahl, N.V.,
and Rizkala, A.R (2007). Ferric gluconate is highly efficacious in anemic hemodialysis patients with high serum and low transferrin saturation: results of the Dialysis Patients Response to IV Iron with Elevated Ferritin (DRIVE) Study. Journal of American Society of Nephrology,(3), 975-984.
Http://kidney.niddk.nih.gov,kudiseases/pubs/hemodialysisdose
Recommendations for Anemia in Chronic Kidney Disease. Retrieved August 25, 2011 from http://www.kidney.org/professional/kdoqi/guidelines_anemia/cpr32.h tm
References
KDOQI Clinical Practice Guidelines and Clinical Practice
Recommendations for Anemia in Chronic Kidney Disease: 2007 Update of Hemoglobin Target. Retrieved August 25, 2011 from http://www.kidney.org/professional/kdoqi/guidelines_ane mia/cpr21.htm KDOQI Clinical Practice Guidelines and Clinical Practice Recommendations 2006 Updates for Hemodialysis Adequacy and Peritoneal Adequacy. Retrieved August 16, 2011 from http:/www.kidney.org/professional/kdoqi/guidelines/guid eline_update HD_PD/pd_guide2.htm
Referrences
Ly, J., Marticorean, R., Donnelly S., (2004). Red blood
cell survival in chronic renal failure. American Journal of Kidney Diseases, 44(4), 715-719.
National Kidney Foundation (2001). KDOQI clinical
practice guidelines for hemodialysis adequacy. American Journal of Kidney Diseases, 37, supp 1, S7-64.
References
Yavuz, A., Tetta, C., Ersoy, F., Dinitin, V., Ratanaret, R.,
De Cal, M., Borello, M., Bordoni, V., Savatori, G., Andrikes, E., Yakapoglu, E., Levin, N., & Ronco, C., (2005) Uremic toxins: A new focus on an old subject. Seminars in Dialysis, 18 (3), 203-212.