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Claude Bernard
“After carrying out an analysis
of phenomena, we must
always reconstruct our
physiological synthesis so as
to see the joint action of all the
parts we have isolated.”
PHYSIOLOGY and LIFE
1. Solid component
Cell
Tissues - master tissues
- vegetative tissues
Organs
Systems
1. Liquid component
2 Major fluid compartments
FLUID COMPARTMENTS
ECF ICF
7.4-7.45 pH 7.35-7.4
308 mOsm/L Osmolarity 308 mOsm/L
1. Reflex in nature
2. Operates on a feedback
mechanism, mostly (-); rarely
(+)
3. Amplification or Gain
4. Oscillation – driving, waxing,
damped
CELL and its FUNCTIONS
Others
Phosphatidylglycerol
Phosphatidylinositol
cardiolipin
Cholesterol
Glycolipids
LIPID BILAYER
Features
1. It is fluid and not solid so portions of
the membrane can flow literally from
one point to another
2. Substances dissolved or floating in the
lipid bilayer diffuse to all areas of the
cell membrane
3. Lipid soluble substances easily cross
the lipid bilayer
4. Water soluble substances do not
dissolve in the lipid bilayer; they cross
the membrane through water-filled
channels, pores, or carriers
2 MEMBRANE PROTEINS
a. Mostly Glycoproteins
b. Fibronectin – integrins
2 types of Proteins
1. Integral proteins
2. Peripheral proteins
MEMBRANE PROTEINS
Functions
1. Anchor
2. Pumps
3. Carriers
4. Ion channels
5. Receptors
6. Enzymes
7. Antibody processing
MEMBRANE CARBOHYDRATES
Functions
1. Bring (-) charge to the cell
2. Attachment of one cell to the other
3. Act as receptor substances for
binding hormones
4. Immune reactions or antigens
CYTOPLASM and its
ORGANELLES
Cytosol is the clear fluid of the
cytoplasm where the particles are
dispersed
It contains mainly dissolved proteins,
electrolytes and glucose, secretory
granules and the organelles
1. Endoplasmic Reticulum (ER)
a. Granular ER
b. Agranular ER
2. Golgi Apparatus
3. Lysosomes
ORGANELLES
4. Peroxisomes
5. Mitochondria
Other structures include:
a. Filaments
• 13 tubulin protofilaments
• Myosin I, II, MTOCs
a. Tubules
∀ α ,β ,δ tubulins
• Kinesia
• Dynein
a. nucleus
INTERCELLULAR
CONNECTIONS
1. Tight Junctions
Tight
Leaky
1. Gap Junctions
CELLULAR TRANSPORT
SYSTEM
A. Transport across but NOT through
Membranes
1. Endocytosis
Energy requiring
Receptor-ligand complex
Sample:cholesterol & LDL receptors
V-SNARES and T-SNARES
2 forms: pinocytosis
phagocytosis
1. Exocytosis
CELLULAR TRANSPORT
SYSTEM
B. Cellular Transport through
Membranes
1. Diffusion
No energy needed
Downhill
2 pathways
Through intermolecular intertices
Through channels or transport
proteins
RATE OF DIFFUSION
Determined by:
1. Amount of substance available
2. Velocity of kinetic motion
3. Range of diffusion – distance
and time
4. Member of openings in the
membrane
DIFFUSION COEFFICIENT
Diffusion Coefficient = D
Proportional to the speed with which
the diffusing molecule can move in
the surrounding medium
Size of molecule
Small molecule 1/(MW1/2)
Larger molecule 1/(MW1/3)
Spherical molecule
Viscosity of the medium
Stokes – Einstein Equation
D= KT / 6π rη
J = (-) DA (∆ c/ ∆ x)
DIFFUSION PERMEABILITY
of the C ELLULAR MEMBRANE
1. Permeable to lipid-soluble molecules
2. Impermeable to water-soluble molecule
Ions are also relatively insoluble in lipid
solvents; diffusion is through protein
channels:
a. Ion specific
b. Allows small ions only
c. Others – diameter, shape, charge
a. Ion channels are controlled by:
a. Voltage difference
b. Chemical or ligand-gated
CELLULAR TRANSPORT THROUGH
MEMBRANE
Osmosis
Semi-permeable membrane
Osmotic pressure
Van’t Hoff’s Law of O.P.
π = RT (φ ic)
Isoosmotic Solutions
Hypoosmotic
Hyperosmotic
PREDICTORS OF VOLUME
CHANGES
1. The steady-state volume of the cell
is determined only by the
concentration of impermeant
solutes in the ECF
2. Permeant solutes cause only
transient changes in cell volume
3. The greater the permeabilty of the
membranes to the permeant solute,
the more rapid the time course
TRANSPORT THROUGH MEMBRANES
Protein-Mediated Transport
Properties
1. More rapid transport
2. Shows saturation kinetics
3. Mediating protein has chemical specificity
4. Structurally related molecules may
compete for transport
5. Transport may be inhibited by compounds
that are not structurally related
In the form of carrier proteins or transporters or
channels
TRANSPORT THROUGH MEMBRANES
Facilitated Transport System
Movement of solutes from an area of higher
concentration to an area of lower
concentration with the use of transporters
No energy required
Can’t move substances against a
concentration gradient
They act to equalize concentrations of the
transported substance on the 2 sides of the
membrane
Show saturation kinetics
Phloretin inhibits sugar uptake while insulin
stimulates it
ACTIVE TRANSPORT