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Criteria &
Standards
Introduction
Quest for pure water
Formulation of specific
standards
To judge the quality of
water
To minimise health
hazards
Guidelines for drinking
water quality (WHO)
Relates to the following
aspects:
Acceptability
Microbiological
Chemical
Radiological
Acceptability
Physical parameters
Inorganic
constituents
Physical Parameters
Biologicalaspects –
Protozoa, Helminths, Free
living organisms
Chemical Aspects
Cumulative toxic properties
Inorganic
Potentially hazardous to human health
Those detected frequently
Those detected in relatively high
concentrations
E.g. Lead, Arsenic, Mercury, Fluoride etc
Organic
Polynuclear aromatic hydrocarbons
Pesticides
Chemical aspects
Health risk assessment
Tolerable daily intake (TDI)
No observed adverse effect
level (NOAEL)
Lowest observed adverse
effect level (LOAEL)
Uncertainty factors
Radiological Aspects
As low as is
reasonably possible
Surveillance of Drinking
water quality
Sanitary survey
Sampling
Bacteriological surveillance
Biological examination
Chemical surveillance
Surveillance of Drinking
water quality
Sanitary Survey
Sampling
For physical
For bacteriological examination
& chemical examination
• Capacity 200-250ml
• Bottle capacity < 2 L.
• Clean, sterile bottles
• Winchester quart bottles
Collection of
samples
Transport &
Surveillance of Drinking
water quality ( contd)
Bacteriological Surveillance
Presumptive coliform test
Colony counts
Cl. perfringens
Biologicalexamination
Chemical surveillance
Presumptive coliform
tests
1. Multiple tube methods
Estimating the most probable no. (MPN) of
colliform organisms in 100 ml of water
the test is carried out by inoculating measured
quantities of the sample water (0.1,1.0,10, 50 ml)
into tubes of Mc Conkey's Lactose Bile
salt broth with Bromocresol purple as indicator
the tubes are incubated for 48 hrs
from the number of tubes showing acid and
gas an estimate of MPN of coliform
organisms in 100 ml of the sample water
can be obtained from the statistical tables
this result is known as presumptive coliform
count (presumption - each tube showing
fermentation- coliform organisms)
sometimes it may be due to other
organisms or combination of organisms
Confirmatory tests
it is not required in case of unchlorinated water,
but required in chlorinated water
done by subculturing each presumptive positive
tube in 2 tubes of brilliant green bile broth, one
of which is incubated at 37 deg C for upto 48 hrs
for confirmation of coliform organisms
other tube is incubated at44 Deg C and
inspected after 6 and 24 hrs to decide whether or
not E. coli is present
E. coli is almost the only coliform organism
which is capable of producing gas from
lactose at 44 deg C
Further confirmation of the presence of
E. coli if desired can be obtained by
testing for Indole production at 44 deg C
2. Membrane Filtration
technique
in some countries used as a standard
procedure to test for coliform organisms
A measured volume is filtered through a
membrane specially made of cellulose
ester
All the bacteria present in water are retained on
the surface of the membrane and by
inoculating the membrane face upwards on a
suitable media and at appropriate temperature,
it is possible to count the colonies and obtain
the results within 20 hrs as compared to72-96
hrs required for the usual multiple tube
technique
Colony count
colony counts on nutrient Agar at 37 deg C and
22 deg C are frequently used in the
bacteriological examination of water
Colony counts provide an estimate of the general
bacterial purity of water
A single count is of little value, but count from the
same source at frequent intervals is of
considerable value- a sudden increase will give
an earliest indication of contamination
Recommended plate
counts
Water at the Plate count Plate count after 3
point of after 2 days atdays at 22 deg C
consumption 37 deg C
Disinfected 0 20
not 10 100
disinfected
Recent studies indicate that a bacterial
plate count on yeast extract agar after
incubation at 22 deg C for 7 days might
serve as a general purpose indicator of
microbial quality because in the absence
of chlorine residual the no. of bacteria can
grow enormously.
Hardness of Water
Soap destroying power of water
Caused by dissolved compounds of
calcium and magnesium
Expressed in terms of mEq./L (< 1=
soft & > 6= very hard water)
Drinking water should be moderately
hard
Disadvantages:
Consumes more soap , Destroys fabrics
Unsuitable in certain industries, Scaling of
utensils / boilers
Shortens life of pipes & fixtures
Advantage - ? Protection from cardio-
vascular diseases
WATER POLLUTION
Two major classifications