ENZYMOLOGI

Difinisi
Enzim adalah protein katalisator yang spesifik.
Katalisator ?
Spesifik ?

Struktur Enzim
NOMENKLATUR DAN
KLASIFIKASI
MULA-MULA SESUAI KEHENDAK
PENEMUNYA: PTYALIN.
DITAMBAH ASE DIBELAKANG SUBSTRAT
PROTEASE = ENZIM PEMECAH PROTEIN
SISTIM INTERNASIONAL I.U.B.S (International Union of
Biochemistry System)
KETENTUAN IUBS
1. ENZIM TERDIRI DARI 6 KELAS UTAMA YAITU:
(1.OKSIDO REDUKTASE, 2.TRANSVERASE,
3.HIDROLASE, 4.LIASE, 5.ISOMERASE, 6.LIGASE.
2. NAMA ENZIM TERDIRI DARI 2 BAGIAN, BGN I
MENYATAKAN SUBSTRATNYA, BGN II DIAKHIRI
ASE MENYATAKAN JENIS REAKSI YG
DIKATALISANYA : LAKTAT DEHYDROGENASE.
3. ADA KETERANGAN TAMBAHAN (DALAM
KURUNG)
4. SETIAP ENZIM PUNYA NOMER KODE SENDIRI2.
E.C (ENZYME CODE) YG TERDIRI DARI 4 DIGIT

Oxido reductase
Enzyme oxido reductase are classified into 4
groups:
1.Oxidases
2.Dehydrogenases
3.Hydroperoksidases
4.Oxygenases
Oxidases
Use oxygen as hydrogen acceptor.
Catalyse the removal of hydrogen from a
substrate.
They formed water or hydrogen peroxide.
AH
2
+ O
2
A + H
2
O

AH
2
+ O
2
A + H
2
O
2

Some oxidases contain copper.
Cytocrome oxidase is hemoprotein, have typical
heme prostetic group present in Hb, myoglobin,
and other cyt.
React hydrogen to oxygen in respiratory chain.
Poisoned by CO, Cyanide, H
2
S
Term as Cytochrome a
3
.
Protein + cyt a + cyt a
3
Cyt aa
3,
it contains
two mol of heme. Cu associated with heme unit.
Flavo protein
Contain flavin mononucleotide (FMN) as
prostetic group.
FMN and FAD formed in the body from vit.
Riboflavin.
Bound tightly to respective apoenzyme protein.
Many flavoprotein enzyme contain one or more
metal as essential cofactors metalloflavo
protein
E.g: Xantin oxidase
Glucose oxidase
An FAD spesific enzyme prepared from
fungi
It use in estimating glucose
Mechanism are complex.
Evidence points is reduction of the
isoalloxacine ring via semiquinone(free
radical) intermediate.
DEHYDROGENASES
CANNOT USE OXYGEN AS A HYDRGEN
ACCEPTOR.
There are large number enzyme
Two main functions are:
1.Transfer of hydrogen from one substrat to
another in couple of redox reaction.
2. As componen of respiratory chain
HYDROPEROXIDASES
Use hidrogen peroxide or organic peroxide
as substrate
Two type of enzyme : peroxidase and
catalase. Both found in animal and plan.
Protect the body againts harmful
peroxsides
OXYGENASES
CATALYSE the direct transfer and
incorporation of oxygen into a substrate
molecule.
It take place in two steps:
1.O
2
binding to enzyme at the active site
2. The bound oxygen reduce or transfered
to substrate
DISTRIBUSI ENZIM DLM SEL
KOMPONEN ENZIM RANTAI
RESPIRASIOKSIDASI FOSFORILASI
TERLETAK PADA MEMBRAN
MITOKONDRIA SEBELAH DALAM.
ENZIM2 SIKLUS KREBS TERDAPAT
DALAM MATRIX MIT.
ENZIM2 PENCERNAAN TDP DLM
LISOSOM.
Bagaimana Enzim Bekerja?
Bagaimana enzim kerja?
SIFAT ENZIM
Tetap struktur dan fungsi di akhir reaksi
Spesifik
Mengalami sintesis dan degradasi

FAKTOR-2 YG MEMPENGARUHI KECEPATAN
KATALISA ENZ.
1. KONSENTRASI SUBSTRAT
2. KONSENTRASI ENZIM
3. SUHU
4. Ph
5. OKSIDASI
6. INHIBISI






Mengapa belajar enzim?
1. Patofisiologi Penyakit
2. Penegakan diagnosis



Sekian