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Microbiology
Manufacturing II
Prof.: Jacqueline Hernández
objetivo
Estudiar ciertos aspectos de la
microbiología enfocados desde el
punto de vista industrial.
Se hará énfasis en la importancia y
utilidad de algunos microorganismos
en procesos industriales.
microbiology
The study of Fungi - single &
microscopic multi cell forms -
organisms which yeast,
include many life- filamentous
forms: molds, complex
Bacteria - simple, fungi
single cell
microbiology
Protists - single cells, Viruses - acellular,
some multicellular protein-based
algae, protozoans, lifeforms,
slime molds typically
intracellular
parasites
mircobiology
Microbiology is not a new field of study.
In 1674 Anton van Leeuwenhoek used
simple, self-made microscopes to examine
stagnant H2O, where he observed "wee
animalcules" - microscopic organisms.
Animalcules“ - microscopic organisms.
microbiology
Hisdetailed descriptions and drawings of
these microscopic forms of life were
published by the Royal Society of
London.
Many others also made significant
contributions including Louis Pasteur,
Edward Jenner and Robert Koch as a few
examples.
Areas of Microbiology
Comparación en Reinos
Biológicos
Haeckel (1894) Whittaker (1969)¹ Woese (1977) Woese (1990)
Tres reinos Cinco reinos Seis reinos Tres dominios
Archaebacteria Archaea
Plantae Plantae
Prokaryotic Cells
Prokaryotic Cells
lack nucleolus.
2. Complexity - No organelles.
3. Cell walls
All typical prokaryotic cells possess cell
walls.
Cell walls made of a peptidoglycan layer.
hrs.)
Population constantly doubles - increases
exponentially
Growth of Micro-organisms
2. Measuring Bacterial Growth
Plate count (standard plate counts)
Direct microscopic count
Turbidity / optical density
Growth of Micro-organisms
3. Phases of
Growth
Micro-organisms
move through
four stages of
growth including
lag, logarithmic,
stationary and
death phases
Growth of Micro-organisms
Lag Phase:
increased metabolic activity
no increased number of cells
cells are preparing for reproduction and
accumulating nutrients and energy
Exponential / Logarithmic Growth Phase
(Log Phase)
microbial population is constantly doubling -
rapid growth / reproduction
diagnostic testing and antibiotic therapies most
effective in this phase
Growth of Micro-organisms
Stationary Phase
population numbers are static: rate of
reproduction = rate of death
occurs due to exhaustion of nutrients,
accumulation of wastes, etc..
Death Phase
rate of death higher than rate of
reproduction.
rate of death is species-variable
Growth of Micro-organisms
Nutrients - fundamental nutritional
requirements include Carbon,
Nitrogen, Hydrogen, Oxygen as well as
trace elements and minerals.
There may be a requirement for special
growth factors for more fastidious
organisms.
Theserequirements could include
vitamins, amino acids, carbohydrates,
blood factors
Parameters Influencing Bacterial Growth
Techniques used to Classify
Bacteria
Bacteria represent a significant source of
pathogenic infections in humans.
There are many ways to classify bacteria.
Examples include:
cell morphology - size and shape (rods,
spheres, spiral)
colony shape (on petri plates)
stain uptake (use of characterizing stains- i.e.
Gram stains)
Techniques used to Classify
Bacteria
motility (mobile / immobile)
energy sources (autotrophs, heterotroph, etc.)
growth requirements (pH, salinity , oxygen,
nitrogen)
products (fermentation / growth products)
antibiotic response and sensitivities
Cell wall composition.
Nucleic acid profiling (sequence comparisons)
Serotyping and immunological testing
RFLP analysis and genetic hybridization
Enumeration of Cells
Numbers of microorganisms can be estimated
based using a number of techniques which
include conducting representative cell
counts (on plates or other media).
Direct microscopic counts are conducted by
examining a known volume of cell suspension
under a microscope.
Direct microscopic counts enumerate living and
dead cells equally.
The use of stains (i.e. Gram stain, etc.) can
increase visibility and allow for both
qualitative and quantitative assessments.
Streak PLate
Standard Plate Count
The SPC (standard plate count) is still the
single most common method of
enumerating cells.
A precise volume of liquid (media /
sample) containing the cells is placed on
the surface of an agar-containing petri
plate.
Standard Plate Count
The plate is incubated (typically >24 hours)
and the plate is counted for colonies.
Each colony is assumed to be derived from a
single bacterial cell - allowing the
microbiologist to relate the number of
colonies (CFU - colony forming unit) and
dilution factors to determine the original
number of organisms in the sample.
This is usually expressed as CFU / ml (colony
forming units per milliliter).
Serial dilution
Serial Dilutions
Alternatively, changes in turbidity can be
measured to assess growth of bacteria in
clear medias / broth.
This can be conducted using a
spectrophotometer or a tubidometer and
provides rapid data.
Optical techniques however, do not
typically differentiate between live and
dead cells.
pensamiento
Amor Fraterno:
Si nos amamos
unos a otros , Dios
vive en nosotros ”.
1Juan - 4 ; 12