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Principle:
Laboratory diagnosis of intestinal amoebiasis is
based on demonstration of haematophagous
trophozoite, cyst or antigen from stool.
Laboratory diagnosis of hepatic amoebiasis is
practically done by detection of trophozoite from
pus or antigen detection from blood and saliva.
Laboratory diagnosis
Culture: Culture of stool samples followed by
isoenzyme analysis can accurately distinguish E.
histolytica from E. dispar and is considered to be
the 'gold standard' for diagnosis.
However, this method takes several weeks to carry
out and requires special laboratory facilities,
making it impractical for routine laboratory test.
Laboratory diagnosis
Immunological tests:
Antigen detection.
Antibody detection.
Enzyme immunoassays (EIA)
Nucleic acid based technique PCR-based
methods.