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institutes
Public health
Local health departments
and CDC
FDA, FSIS/USDA
Non-regulatory
National disease surveillance
Outbreak detection and
Regulatory
Food safety policies
Inspect, monitor, enforce
Product recall and traceback
Investigation of farm and
production facilities
Problem identification
Problem management
regulatory action
FoodNet
Reports trends in
foodborne infections and
tracks the impact of food
safety policies nationally
Developed: 1995
Because: After the 1993 outbreak due to E. coli O157, USDAs
Food Safety Inspection Service revised meat inspection system.
To tell Congress if E. coli O157 infections were being prevented,
they helped CDC start a new tracking system.
Now: Conducts surveillance for 9 infections and hemolytic
uremic syndrome (HUS), working closely with 10 state health
departments and other federal agencies.
Annual report card on food safety..
Since 2006-2008,
only E. coli O157 has
decreased significantly
http://www.cdc.gov/foodnet/trends/figures-2014.html#ui-id-3
by other routes
We investigate outbreaks to
Investigating an outbreak
Detect that an outbreak may be happening
Develop a hypothesis about how transmission
occurred
Test the hypothesis 3 lines of investigation
Epidemiological investigation
Source tracing
Culture of suspect food items
The regulatory authorities and the affected company
This is an
infection with
Campylobacter bacteria
Campylobacter, eh?
We can treat that, and we
need to report it to the
local health department
I will test it for antibiotic resistance
Local/State Health
Department Level
Campylobacter infections
1.3 million illnesses, 13,000 hospitalizations, and 120
deaths annually in the U.S.
Acquired from different sources
bird and cattle reservoirs
poultry, raw milk, animal contact, surface water
Causes diarrheal illness in humans
bloody and non-bloody diarrhea
Guillain Barr Syndrome (1/1000)
Mat-Su Valley
Anchorage
Kenai
Peninsula
Illness features:
91% diarrhea
82% abdominal cramps
21% bloody diarrhea
In all: 98 cases identified
5 hospitalized, 1 with Guillain Barre syndrome
Hypothesis generation: initial interviews
Some spontaneously mentioned eating fresh raw peas
Gardner 2011 Clin Infect Dis 53:26-32
Farm A Peas
Farm A
To process:
Combine with
wash tank
2009: Scare-cranes,
authorities
Often associated with a particular event
Dispersed outbreaks were rarely detected
Fostered impression that foodborne disease was just a
local problem
outbreaks
Dispersed outbreak
themselves
surveillance
Multi-state investigation
Local investigation
Industrial contamination event
Local food handling error
Local solution
Broad implications
PulseNet
Connects cases of
illness nationwide to
identify outbreaks that
would otherwise go
undetected
Started: 1996
Because: After 1993 E. coli O157 outbreak (726 cases, 4 deaths),
many clinical labs began testing for E. coli O157. CDC began
PulseNet to detect outbreaks sooner.
Now: National network of public health and food regulatory agency
laboratories that perform standardized molecular subtyping
(fingerprinting) of E. coli O157, Salmonella and Listeria
PulseNet staff
search for similar
patterns in past 2-4
months
When a cluster is
identified, they
report it to
epidemiologists
States can look at
PulseNet database
too
Local/State Health
Department Level
Local/State Health
Department Level
National
Level = CDC
Local/State Health
Department Level
National
Level = CDC
Well hold a conference
call with all the states that have
cases. Let us know if your
interviews give any clues
Salmonella infections
1.2 million illnesses, 23,000 hospitalizations, and 450
deaths annually in the U.S.
Acquired from many different sources
Many animal reservoirs
Poultry, meat, fresh produce, eggs, raw milk,
animal contact
Causes diarrheal illness in humans
blood stream infections
severe focal infections
Many serotypes: Enteritidis, Typhimurium, Newport and
Heidelberg are most common
55% female
Median age 21 years
31% hospitalized
No deaths
2/14 tested were multidrug resistant, including
ceftriaxone
October 8, 2013:
Results of patient interviews (n=132)
Case-Patients
Comparison
Exposure
# Exposed/
# Observed
Percent
Exposed
Chicken
105/132
80%
64%
0.002
48/61
79%
NA
NA
Foster Farms
brand
P-value
Outbreak summary
49% female
Median age 18 years
38% hospitalized
13% blood infections
No deaths
Bagged spinach
Carrot juice
Peanut butter
Broccoli powder on a snack food
Dry dog food
Frozen pot pies
Canned chili sauce
Hot peppers
White and black pepper
Raw cookie dough (flour?)
Hazelnuts
Fenugreek sprouts
Papayas
Pine nuts
Raw scraped tuna
Pomegranate anils
Torshi
Cashew nut cheese
Cucumbers
Chia sprout powder
Caramel-dipped apples
If detecting a
dispersed outbreak
is like finding a
needle in the
haystack,
would a bigger
magnet help?
The Opportunity
Listeria monocytogenes
CDC warned
o Public, with web-updates, social
media, media outreach
o All health care facilities
o All public health departments
There is a small
cloud on the
horizon
Rapid culture-independent
diagnostic tests
2013
Luminex
GI Pathogen Panel
15 targets,
five hours
Multi-pathogen
culture independent
diagnostic platforms
2015
BioFire FilmArray
22 targets,
one hour
PulseNet fingerprints
Culture-independent
diagnostic test platform
No PulseNet fingerprints,
No serotype, no antibiotic
susceptibilty test, no DNA sequence
PulseNet fingerprints
Reflex culture
Culture-independent
diagnostic test platform
No PulseNet fingerprints,
No serotype, no antibiotic
susceptibilty test, no DNA sequence
Long term: Develop assays for public health that are themselves
culture-independent
Selective amplification around diagnostic regions of genome?
Single cell sorting and sequencing?
Of use in food matrices as well?
Thank you