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Fisiologi Pertumbuhan
Kurva Pertumbuhan
Respirasi
Pertumbuhan Mikroba
Kontrol pertumbuhan:
infeksi
Industri dan bioteknologi
Chemical Requirements
#1 = air
Elements
C (50% of cells dry weight) H, O, N, P, S
mineral
Organik
Sumber energi glukosa
Vitamin (koenzim)
Beberapa asam amino purin dan
pirimidin
Environmental Factors
Influencing Growth
Temperature
O2
pH
Osmotic Pressure
Others: radiation, atmospheric
pressure
Bacterial Division
Bacteria divide by binary fission
Alternative means
Budding
Conidiospores (filamentous
bacteria)
Fragmentation
Fig. 7.13
Generation Time
Time required for cell to divide/for
population to double
Average for bacteria is 1-3 hours
E. coli generation time = 20 min
20 generations (7 hours), 1 cell
becomes 1 million cells!
Phases of Growth
Lag phase making new enzymes
in response to new medium
Log phase exponential growth
Desired for production of
products
Most sensitive to drugs and
radiation during this period
Phases of Growth
Stationary phase
nutrients becoming limiting or
waste products becoming toxic
death rate = division rate
Death phase death exceeds
division
Measuring Growth
Direct methods count individual
cells
Indirect Methods measure effects
of bacterial growth
RESPIRASI
Aerob
Anaerob
Fermentasi
Figure 9.UN05
Figure 9.6-1
Electrons
carried
via NADH
Glycolysis
Glucose
Pyruvate
CYTOSOL
ATP
Substrate-level
phosphorylation
MITOCHONDRION
Figure 9.6-2
Electrons carried
via NADH and
FADH2
Electrons
carried
via NADH
Glycolysis
Glucose
Pyruvate
CYTOSOL
Pyruvate
oxidation
Acetyl CoA
Citric
acid
cycle
MITOCHONDRION
ATP
ATP
Substrate-level
phosphorylation
Substrate-level
phosphorylation
Figure 9.6-3
Electrons carried
via NADH and
FADH2
Electrons
carried
via NADH
Glycolysis
Glucose
Pyruvate
CYTOSOL
Pyruvate
oxidation
Acetyl CoA
Citric
acid
cycle
Oxidative
phosphorylation:
electron transport
and
chemiosmosis
MITOCHONDRION
ATP
ATP
ATP
Substrate-level
phosphorylation
Substrate-level
phosphorylation
Oxidative
phosphorylation