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Professor B. Z. Chowdhry
School of Science
Room 202, Grenville Building
Tel: 0208-331-8208
E-mail: b.z.chowdhry@gre.ac.uk
What about:
High energy phosphate compounds
Low energy phosphate compounds ?
Concept of high energy and low energy
phosphates not useful!
Phosphoenolpyruvate (PEP)
61.9
Phosphocreatine
43.1
Pyrophosphate
33.5
30.5
Glucose-6-phosphate
13.8
Glycerol-3-phosphate
9.2
THE FACTS
(2)
(3)
(4)
(5)
(ii)
(iii)
the synthesis of acetylcoenzyme A in bacterial
cells.
CH3COOPO32- + ADP3-
CH3COSCoA + HOPO32-
Answer
In any set of coupled reactions the elements of water (not a
molecule of water as such) are removed from the reactants of
the condensation and incorporated into phosphate.
If we make allowances for ionisation an OH group is removed
from acetic acid (CH3CO2-) and liberated as part of ADP whilst
H+ is removed from coenzyme A and liberated as part of
phosphoric acid.
The formation of the condensation product is therefore
facilitated because water is being removed and liberated in the
form of a species which has mole fractions less than unity!
Vitamin
source
Mechanistic role
Coenzyme
Adenosine triphosphate
Transfer of phosphoryl
or
nucleotidyl groups
Cosubstrate
Metabolite Coenzymes:
Synthesized from common metabolites
Include: e.g. ATP
Methionine + ATP
adenosylmethionine + PPPi
(S-AM)
Norepinephrine
CH2
CH2
+
NH3
HO
HO
Epinephrine
HO
CH2
CH2
+
NH2
CH3
Topic 2
Protein phosphorylation
Protein phosphorylation
Protein + ATP
Active
E1
Protein-P +ADP
Inactive
OR
Protein + ATP
Inactive
E2
Protein-P +ADP
Active
Signalling networks
Can be very complex
E.g. protein A phosphorylates protein B
Protein B phosphorylates C
BUT
A can also phosphorylate C directly
B can phosphorylate D, which may in turn phosphorylate A