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ANJALI
ESEM 1st SEMESTER
WHAT IS
CHROMATOGRAPHY?
TYPES OF CHROMATOGRAPHY
Gas chromatography
Paper chromatography
Thin layer chromatography (TLC)
High performance liquid chromatography
(HPLC)
GAS
CHROMATOGRAPHY
PRINCIPLE
Flow controller
column
Waste
Detector
Carrier
gas
Column oven
APPLICATIONS
ADVANTAGES &
DISADVANTAGES
PAPER CHROMATOGRAPHY
TYPES
DESCENDING PC
In this type, development of the chromatogram is done by allowing
the solvent to travel down the paper. Mobile phase is present in the
upper portion.
ASCENDING PC
Here the solvent travel upward direction of the chromatographic
paper.
ASCENDING-DESCENDING PC
It is the hybrid of both the above technique. The upper part of the
ascending chromatography can be folded over a rod & allowing the
paper to become descending after crossing the rod.
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HORIZONTAL PC
Here a circular paper is taken & the sample is
given at the center of the paper. After drying the
spot the filter paper tied horizontally on a
petridish containing solvent. So that wick of the
paper is dipped inside the solvent. The solvent
rises through the wick & the component get
separated in form of concentrate circular zone.
TWO DIMENSIONAL PC
In this technique a square or rectangular paper is
used. Here the sample is applied to one of the
corners & development is performed at right
angle to the direction of the first run.
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APPLICATIONS
ADVANTAGES &
DISADVANTAGES
Large quantity of
sample cannot be
applied.
In quantitative
analysis it is not
effective.
Complex mixture
cannot be
separated.
Less accurate.
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THIN LAYER
CHROMATOGRAPHY
Thin layer
chromatography
plate
Beaker
Pencil line
Spot of mixture
Solvent
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PRINCIPLE
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APPLICATIONS
ADVANTAGES &
DISADVANTAGES
Pump to
produce
high
pressure
Sample
injectio
n
Signal to processor
HPLC tube
Detector
waste
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TYPES
APPLICATIONS
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ADVANTAGES &
DISADVANTAGES
Speed (minutes)
High resolution
Sensitivity
Reproducibility of +/1%
Accuracy
automation
Cost
Complexity
Low sensitivity for
some compounds
Irreversibly adsorbed
compounds not
detected.
Coeluction difficult
to detect
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THANK YOU
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