The Guide

Feb 2012

Tissue
Culture

Fun
Fruit
Nutrition

Biotechnology

By:
Afida Zahara Adzkiya
Alifah Farah Gusmaniar
Indah Puspita Aziz
Ridha Astri Indryana

1987 Street Ave City,

State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com

Fun
Fruit
Nutrition

Definition of tissue culture

Tissue
culture
is
an
activity to cultivate a tissue of
plant vegetatively to be a
plant
that
has
similar
property to its parent in a
short time.
Plants can be multiplied
using this technique as plants
have a totipotency ability,
meaning that from a tissue
they can grow and develop
into new individuals. Tissue
culture is used to solve food
shortage problems.
1987 Street Ave City,
State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com

Fun
Fruit
Nutrition

Information Layout Slide with Table

Tissue culture was found and

well known by F.C Steward, a plant
physiologist
Who

did

from

his

United

research

in

States.
1989.

Steward try to make tissue culture

of carrot and he is success.
Tissue culture well because the
work of Muer, Hildebrant, and
Riker in 1954.
1987 Street Ave City,
State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com

Fun
Fruit
Nutrition

The Various Uses of Tissue Culture

Obtaining a lot of
germs in a short time
with similar property to
the parent.
Making plants with
properties we want.
Tissue culture is
economical from
aspect of time , space,
and energy.
Produce plants free
from virus and disease.
1987 Street Ave City,
State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com

The Procedure of Tissue Culture

Technique

1987 Street Ave City,

State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com

Preparing
growth
medium.
Substance that must exist
in growth medium consist
of
minerals
(macro
element, such as C, H, O, N
and micro element such as
Zn, Mo), amino acid,
vitamin,
sugar,
and
hormone
in
certain
amount.

1987 Street Ave City,

State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com

Preparing tissue.
The tip part of a young shoot, such as
root and leaf tips, are cut into 1-1.5
mm. Next, the tissue is sterilized
using 5% hypochlorite calcium and is
washed off several times with sterile
aquadest.

1987 Street Ave City,

State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com

Cultivating tissue
(explants) in liquid growth
medium.
Explants are placed in a mixer table (shaker) that
shakes at 60-70 times per minute. The mixing is
performed for 6 hours a day for 1.5 2 months .
Shaking is meant for faster distribution of substance
and food absorption as well as process of gas
exchange. In about 2 months, callus will grow in this
liquid medium.

1987 Street Ave City,

State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com

Moving plant
whose root
bud and leaf
bud have
grown to
another solid
growth
medium.

1987 Street Ave City,

State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com

Cultivating
plant
in
pot
containing soil.
The plants whose rot bud and
leaf bud have grown are
removed
to
another
solid
medium to be separated, so
bigger plants can grow.

1987 Street Ave City,

State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com

Cultivating plant in
actual land.
Before being cultivated in actual land,
the plant from solid medium is moved
or cultivated first in a pot that contains
soil that has been given fertilizer. If the
condition of plant has been strong, it
can be moved to actual land.

1987 Street Ave City,

State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com

Question Time
Apakah objek yang dikultur oleh
pempopuler tekhnik kultur jaringan (Muer,
Hildebrant, and Riker) sama dengan objek
yang dikultur oleh F. C. Steward?
Tidak sama.
Hildebrant berhasil mengembangkan tekhnik kultur jaringan pada selsel rambut dari jaringan mesofil daun tanaman monokotil.
Muer berhasil menumbuhkan tanaman lengkap dari kultur sel
tunggal.
Kalau Riker kami belum mengetahui apa objek yang dikulturnya,
namun pada logikanya itu pasti berbeda dengan objek yang dikultur
oleh F. C. Steward.

1987 Street Ave City,

State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com

Question Time
Question from Sabila:
What kind of plant that usually
used for tissue culture?

Pada dasarnya semua jenid tumbuhan dapat dikultur, karena

semua tumbuhan memiliki sifat totipotensi di jaringan meristem
(ujung akar dan batang). Namun tumbuhan yang biasanya
dikultur adalah:
-Yang pertumbuhannya lambat atau susah
-Memiliki nilai jual yang tinggi
-Banyak bergunan bagi masyarakat
-Contohnya adalah tanaman anggrek, jati dan tebu.

1987 Street Ave City,

State
60623, US.

Balanced Nutrition Coming to Fruition

www.funFruit.com