Академический Документы
Профессиональный Документы
Культура Документы
&
LAMBDA PHAGE CYCLE
Bhaskar Ganguly
Ph.D. , M.V.Sc., B.V.Sc. & A.H.
Examples of cofactors
common prosthetic groups (haem) or metal ions
(magnesium)
Dissociable protein subunits sigma () factor.
General Properties of
factor
TTGACA
TATAAT
Sigma factors
Sigma subunit
70 RpoD
Growth/housekeeping
54 RpoN
~15
RpoS
~100
RpoH
Heat shock
~40
RpoF
Flagella-chemotaxis
~40
~5
~5
32 RpoE
FecI
# of genes controlled
~1000
In log-phase E. coli:
~4000 genes
~2000 core RNA polymerase molecules
~2/3 (1300) are active at a time
~1/3 (650) can bind subunits.
The alternative
54
factor
Lysis or Lysogeny
Lysis: Infection by phage produces
many progeny and breaks open (lyses)
the host bacterium
Lysogeny: After infection, the phage
DNA integrates into the host genome
and resides there passively
No progeny
No lysis of the host
Can subsequently lyse (lysogeny)
UV
Induction
Lysis
Lysogeny
Elements of lysogeny
The phage genome integrated into the
host bacterial genome is a prophage.
Bacterium carrying the prophage is a
lysogen.
Lysogens are immune to further infection
by similar phage because the phage
functions are repressed in trans.
Induction of the lysogen leads to
excision of the prophage, replication of
the phage DNA, and lysis of the host
bacterium.
int
gam
red
xis cIII N
Pint
tL1
cI
cro
cII O P Q
Virus head
Lysis &tail
SR
AJ
promoter
operator
terminator
Not to scale!
Immediate early
transcription
int
gam
red
xis cIII N
Pint
tL1
cI
cro
cII O P Q
SR
tR2 PR t6S
tR3
6S RNA
Cro
AJ
int
gam
red
xis cIII N
Pint
tL1
PL
N
cI
cro
N
cII O P Q
SR
AJ
tR2 PR t6S
tR3
6S RNA
N protein
CIII
Recombination proteins
Cro
CII
Q protein
Replication proteins
int
gam
red
xis cIII N
Pint
tL1
Cro
cI
cro
Q
cII O P Q
SR
AJ
tR2 PR t6S
tR3
Lytic functions
Replication proteins
Viral head & tail proteins
int
gam
red
xis cIII N
Pint
tL1
Cro
cI
Q
cro
cII O P Q
SR
AJ
tR2 PR t6S
tR3
Lytic functions
Viral head & tail proteins
att
int
gam
red
xis cIII N
tint Pint
Int
CIII
CII
tL1
cI
cro
CII
cII O P Q
CI
Repressor
SR
AJ
tR2 PR t6S
tR3
PRE = promoter for
repression
establishment
att
int
gam
red
xis cIII N
Pint
tL1
CI
cI
cro
cII O P Q
CI
Repressor
SR
AJ
tR2 PR t6S
tR3
PRM = promoter for
repression
maintenance
Activated by Repressor
binding to oR1 & oR2
Repressor structure
repressor is a dimer; monomer has 236 amino acids.
C-terminal domain: protein-protein interaction;
dimerization and cooperativity
Connector
N-terminus: DNA binding; Helix-Turn-Helix motif
operator
operator
oR2
operator
oR1
Thank you