Академический Документы
Профессиональный Документы
Культура Документы
SIZE OF BACTERIA
Cocci: sphere, 1m
Bacilli: rods , 0.5-1 m in width -3 m in length
Spiral bacteria: 1~3 m in length and 0.3-0.6 m in width
Structure of Bacteria
Essential structures
cell wall
cell membrane
Cytoplasm
nuclear material
Particular structures
capsule
flagella
pili
spore
1884: Christian Gram: First publication for the Gram stain method)
Editor's note: I would like to testify that I have found the Gram method to be one of
the best and for many cases the best method which I have ever used for staining
Schizomycetes.
Flagellum
Cell membrane Nucleoid
Cell wall
Gram +
Pili
Gram Granule
Capsule
Cell (inner) membrane Outer membrane
Ribosomes
Cell wall
Cell wall
Situation:
outmost portion.
15-30nm in
thickness, 10%25% of dry
weight.
Special components of
Gram positive cell wall
Teichoic acid
SPA / M POTEIN
Wall-less forms of
Bacteria.
When bacteria are treated with 1) enzymes that are lytic for
Cell
membrane
Site of biosynthesis of DNA, cell wall polymers and membrane lipids. Selective
permeability and transport of solutes into cells
Electron transport and oxidative phosphorylation
Excretion of hydrolytic exoenzymes
Mesosomes
Cytoplasm
Composed
extrachromosomal
genetic elements
Inclusions: sources of stored
energy, e,g volutin
Plasmid
granulo
se
Plasmids are
small circular/line extrachromosomal
double-stranded DNA molecules They
are capable of self-replication and contain
genes that confer some properties such as
antibiotic resistance virulence factors
Plasmids are not essential for cellular
survival.
Inclusions are
aggregates of various
compounds that are
normally involved in
storing energy
reserves or building
blocks for the cell.
Inclusions accumilate
when a cell is grown
in the presence of
excess nutrients and
they are often
observed under
laboratory
Inclusions of
Bacteria
Nucleus
Lacking
nuclear
membrane, absence
of nucleoli, hence
known as nucleic
material or nucleoid,
one to several per
bacterium.
Attachment
Protection from phagocytic
engulfment.
Resistance to drying.
Depot for waste products.
Reservoir for certain
nutrients.
protection
These are structures surrounding the outside of the cell envelope. They
usually consist of polysaccharide; however, in certain bacilli they are
composed of a polypeptide (polyglutamic acid). They are not essential to
cell viability and some strains within a species will produce a capsule,
whilst others do not. Capsules are often lost during in vitro culture.
Identification
of Bacteria
Pathogenesis
Motility of
bacteria
Monotrichate/Amphitrichate/Lophotrichate/Peritrichate
Flagella
Pili
Pili
Endospores
(spores)
Dormant cell
Resistant to adverse
conditions
- high temperatures
- organic solvents
Identification of
Bacteria
Pathogenesis
Resistance
Methods
Microscope
Light Microscope
Electron Microscope
Darkfield Microscope
Phase Contrast Microscope
Fluorescence Microscope
Cofocal Microscope
Staining Methods
Simple
staining;
Differential staining ( Gram
stain, Acid-fast stain),
Special staining( Negative stain,
Spore stain, Flagella stain)