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US\Amel Altayeb
MSC u of k
Last lecture QS
1-GLYCOGEN OCCUR IN TOW
MAIN FORMS??
2- GLYCOGEN FOUND IN GREATEST
AMOUNT IN
3: ALPHA AMYLASE EXTRACT
FROM
CARMINE IS. AND EXTRACTED
FROM
WHAT IS THE PRINCIPLE OF HEXAMIN SILVER
TECHNIQUE??
defintion
Known as
mucopolysaccaride,glycosaminoglyc
an
classification
1:neutral mucins
2:Acidic mucins
ACIDIC MUCIN:
A:Strongly sulfated:
1:Connective tissue
2:epithelail
B:Weakly sulfated:
C:Carboxylated sialomucin:
1:enzyme labile (n-acetyle)
2:enzyme resistant(n-acetyl-o-acetyl)
D:Sulfated sialomucin:
A:Non- sulfated :carboxylated uronic acid
(hyalurnic acid)
Acidic mucin
A:1:Strongly sulfated connective
tissue mucins (proteogylycan):
this mucins react in low PH with
cationic dyes
Its usually PAS (-VE)
Produced by
fibroblast,endothelial
cells,osteocyte,chondrocyte and
mast cells
1:chondrotin sulafate A
2:chondrotin sulafate B
3:chondrotin sulafate c
4:Heparin/heparan sulfate
5:Keratan sulfate
6:Hyaluronosulfate
Chondroitin sulfated
C
Heparin/heparan
sulfate
Keratan sulfate
Cartilage,umblical
cord,and dermis of
skin
Aorta,cardiac
connective
tissue,mast cell
Cornea,ageing
C:Carboxylated
mucins( sialomucins)
1: enzyme labile: N-acetyl
sialomucins:
**can be digested by sialidaze
enzyme.
**found in bronchial sub mucous
gland,submandibular salivary
glands,and goblet cells of small
intestin
2:Enzyme resistant
sialomucin(N-acetyle-oneuramic acid)
Resistant to digestion by sialidase
enzyme
Its PAS negative
Found in the mucosaof the large
intestin, stomach, and bronchus
D:Sulfated sialomucine
Found in large amount in the
prostatic carcinoma, malignant
synovioma
A:hyaluronic acid:
Found in the umblical cord,early
placenta,cardiac connective tissues and
dermis of skin
2:neutral mucins
Its epithelial in type
Most abundant in Brunners gland
1:chitin
Its hyaline substances in non
human tissue
Its forming the exoskeleton of
various insects
In human its usually seen in the
2:starch
Starch may be on the food content
It may enter during the surgical
operations via the medical gloves
leading to formation of granuloma
Its appear as polygonal to round
bodies , with a central refractile
Cellulose
droplets.
Undigested food particles ,can be
demonstrated in the gastrointestinal tract.