CD10 expression in stromal cells of

oral cavity squamous cell carcinoma:a

clinic and pathologic correlation
A Piattelli, M Fioroni, Giezzi, V Perrotti, M
Piattelli, C Rubini
Oral disease 2006

G.Kiran kumar

Aims and objectives

Aim

of the study was a correlation of CD10 and

several parameters : age, tumor size, presence of
lymphnode metastasis, clinical stage, histologic
grading , presence of local recurrences.

CD10

is expressed in majority of follicle center

lymphomas and burkits lymphomas. CD10 also
been shown to be present in variety of other
neoplasms.

Introduction
CD10

is a zinc dependantant
metallopeptidase with a
molecular weight of 90,000 -110
000 .

Biochemical

point of view it is a
type II integral membrane protein
known as neutral endopeptidase
24.11.

 CD

10 is widely distributed and has been found in

Normal and neoplastic hematopoietic and lymphoid

cells.
Renal tubules
Brush border of intestine
Hepatic canaliculi
Fetal liver cells
Normal liver cells
Gonads
Adrenal cortex
Brain
Leukocytes

CD10

may play specific role in the control of cell

growth and differentiation of both hematopoietic
and epithelial systems.

Variety

of neoplasms such as
Renal cell carcinoma, prostatic adenocarcinoma,
transitional cell carcinoma, pancreatic
adenocarcinoma, malignant melanoma etc.

In

skin it might be an indicator of

tumor invasiveness.

In

renal malignancies it is an
additional marker for differential
diagnosis of histological types.

Materials and methods

Sample

size taken was tissues of 77 consecutive

patients with SCC of oral cavity.
Biopsy was taken with the presence of normal
epithelium at the periphery of lesion.

These

tissues were retrieved from archives of

the Department of Pathologic Anatomy and
Histopathology of University of Ancona,Italy.

Immunostaining

was performed with

monoclonal antibody directed against CD10.

Sections

were deparaffinized in xylene and

dehydrated in an alcohol series.

Immunostaining

with CD10 required

pretreatment with EDTA at pH 8.0 for 20min
at 250W in microwave oven.

CD10

was added for 1hr at 37c in a moist chamber

followed by incubation with biotinylated antimouse
IgG.

Avidin

biotin complex and alkaline phosphate

reagent each for 30 min.

Between

each steps sections were washed with Tris

buffered saline.

Fast

red and 3,3 diaminobenzidinetetrahydrochloride

served as chromogens.

Brown

staining of cell membrane was

considered positive.

CD10

expression is evident in the stromal cells

but not in the tumor cells.

When

the neoplastic cells were surrounded by

the stromal expressing CD10, the expression
was considered to be positive.

In

this study if 10% of stromal cells in the

area of invasive growth were for CD10
the case was regarded as having
proliferation of CD+ stromal cells and
defined as CD10+.

STASTICAL EVALUATION
Correlation

between stromal CD10 expression

and clinico-pathologic factors were evaluated
using chi-square test and p value was less than
0.05 considered as significant.

RESULTS
25

out of 77 cases of SCC were positive.

CD10

positive cases of which 16 were

associated with cervical lymphnode metastses
and 14 cases were related to recurrences

CD10

positive was present in 6 cases of

WDSCC, 9 cases of MDSCC and 10 cases of
PDSCC.

Highly

significant corelations were found with

lymph node status, presence of local recurrences
and histologic grading.

Not

correlated with tumor size and clinic stage

Discussion
Contrasting

results have been reported on the

prognostic value of CD10.

In

primary intestinal lymphomas CD10+ group

showed longer survival than CD10- group. (G0
et al 2002).

CD10+

in lung cancer patients correlated with a

significantly higher 5yrs survival than negative
patients.(Tokuhara et al 1991)

Improved

survival rate with CD10+ in diffuse

large B cell lymphoma(Ohshima et al 2001).

But

, Uherova et al said that cd10+ displayed

overall shorter survival time.

Haradr

etal (1999) said that as such there is no

difference found in survival of CD10+ and
CD10- patients.

In

this study CD10+ is an indicator of worse

prognosis.

Positive

cells were correlated with metastases,

local recurrences and histologic grading.

This

article strongly supports the role of CD10

in the differentiation and growth of neoplastic
cells and it may have role in the tumor invasion
probably facilitating the occurrences of
metastases.

Thank you