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maturation)
PRESENTED TO : DR AKHTAR RASOL
PRESENTED BY :
2013-VA-204
2013-VA-206
2013-VA-209
2013-VA-210
IVM
It
Alternative
to IVF
Successful
First
Medium
There
TCM-199
Hams
F12
Assessment of Oocyte
Presence of vacuole.
IVM
1- glass
2- plastic
Temp 39 c
Humidity: 98-100 %
CO2 concentration: 2%
Types of maturation
1- cytoplasmic
2- nuclear
For staining; fixing of oocyte with acetic acid and staining with 1 %
aceto orecin to check nuclear maturation
Step 3.When the eggs are mature, they are fertilised with your
partners, or donors sperm. Embryos are cultured then
transferred to your womb, just as they would be with
conventional IVF treatment.
IVF vs IVM
ICSI
Risk of IVM
Reference