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pGLO
Genetic transformation
PA
Plasmids
PGLO lab
What is GFP
Safety
Clean Up
When all the agar is dissolved, allow the LB nutrient agar to cool so
that the outside of the flask is just comfortable to hold (50C).
While the agar is cooling, label the plates and prepare the arabinose
and ampicillin as outlined below (Step 3). Be careful not to let the agar
cool so much that it begins to solidify. Keeping the flask with molten
agar in a waterbath set to 50C can help prevent the agar from cooling
too quickly.
Add the entire contents of the LB nutrient agar packet. Swirl the flask to
dissolve the agar, and heat to boiling Repeat heating and swirling until
all the agar is dissolved
Prepare Arabinose/Ampicillin
Plate Storage
Arabinose
Arabinose
Transferring E.coli
Heat Shock
Recovery