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The Nature of
Biochemistry
B.
C.
Range of Application
Biochemistry
Branches of Biochemistry
Biochemistr
y
Living
Organism
Biomolecules
Complex
Polysaccharid
es
Polynucleotid
es
Proteins
Lipids
Membranes
Simple
Carbohydrate
s
Nutrients
Kingdoms
Energy
Morphology
Cells
Nucleotides
Amino acids
others
Organelles
Eukaryotic
Prokaryotic
Complex
Simple cells
Nuclear/boun
d DNA
Cytoplasm
DNA
potassium etc.
The organic substances such as
Attributes of Life
METABOLISM
REGULATION
LOCOMOTION
- The ability to move on its initiative, under its control.
VARIATION AND CHANGE
- Which explain why no two organisms are exactly
WATER
- Is more essential to life than is food.
- A person can live for several days or
Functions
1. It is a universal medium in which the various chemical changes of
General Properties
A. Water has a unique properties
-has a boiling point
- freezing point
-heat of vaporization
-specific heat
- heat of fusion
- water tension( the forces of attraction
between
molecules in liquid water is relatively high)
Water Component
Interstitial water
Homeostasis
-
A.Water Intake
1. Fluids and beverages 1,200 ml- 1,500 ml
2. Daily water intake - 2,100 ml 2,800 ml
3. Daily output 1,850 ml 2,600 ml
A.Organs
1.KIDNEY - one liter, 15 ml of water is required to
dissolved 1 gm of solute.
2.SKIN 350 ml of water is lost daily through the
skin by diffusion. 450 ml 700 ml of water is lost
through skin.
3.LUNGS lung water goes out of the body as tiny
droplets in the expired air ( 350 ml)
4.FECES 150 ml , small amount of water is
usually lost daily through intestinal elimination.
Electrolytes
-Acid, base and salt under current process is
known as electrolytes in the process of
ionization.
a. POSITIVE IONS ( CATIONS)
1. Sodium
(Na+)
2. Potassium (K+)
3. Calcium (Ca+)
4. Magnesium (Mg+)
Chloride (Cl-)
Bicarbonate (HCO3 -)
Phosphate (PO4-)
Sulfate (SO4 -)
Ions of inorganic salts such as lactate pyruvate
Organic Compounds
1. Nucleic acid (DNA and RNA)
2. proteins
3. carbohydrates
4. fats and lipids
Inorganic Elements
1. Bulk elements (N,Na,Mg,P,S,Cl,K, and Ca)
Structure of Cell
Cell is a structural & functional unit of human body, capable of
carrying out functions of life independently.
Basic unit of life.
Nucleus
Cytoplasm
Cell Membrane
Functions of Cell
Production of Bio-Energy
- Storage
- Multiplication
- Specific function according to location
Cell Wall
The cell wall functions to support and protect the cell.
Plants have cell walls composed of cellulose; fungi have
walls composed of chitin.
Nucleus
The nucleus contains DNA; it is the control center of the
cell because DNA contains instructions needed to
produce proteins that control metabolism and other cell
functions.
Cytoplasm
Is the material enclosed by the plasma membrane,
excluding the nucleus.
Ribosomes
Synthesize protein accordingly. It also read the code in
mRNA.
Nucleolus
It is a structure within the nucleus where the ribosomal
subunits are produced.
This structure appears darker than the nucleus.
Lysosomes
These are membrane-bound vesicles containing hydrolytic
digestive enzymes produced by the Golgi Complex.
They fused with other vesicles formed around the material
the has entered the cell, allowing the digestion of the
vesicle contents.
Peroxisomes
These are vesicles that contain enzymes which oxidize
(remove hydrogen) from a variety of different compounds
and pass the hydrogen to oxygen.
Vacuoles
It is a membranous sac similar to but larger than
vesicles.
This structure stores water and dissolved substances.
Chloroplasts
It is the site of photosynthesis in the plants.
Mitochondria
It is the powerhouse of the cell where ATP, the energy
currency of the cell is produced.
Cytoskeleton
- It is a network of protein molecules that provides the
Microtubules
The structure responsible for the movement of cilia and
flagella.
They also move the chromosomes during cell division.
Tissues
Classification of Tissues
Connective Tissue
Muscular Tissue
Nervous Tissue
Epithelial Tissue
Systems
Muscular System
Skeletal System
Digestive System
Respiratory System
Circulatory System
Excretory System
Reproductive System (Male & Female)
Nervous System
Endocrine System
Physical Chemistry
Biochemistry dealing as it does with the
COLLOIDAL STATE OF
MATTER
The old method of grouping substances
into colloids and crystalloids was base
upon the ability of the substances to pass
through parchment membrane. Those that
diffuse readily through such membrane
were called CRYSTALLOIDS, while those
which do not were termed COLLOIDS.
General Properties
1. Filtrability
3. Tyndall phenomenon
-colloidal solution manifest the tyndall effect.
That is , the path of a powerful beam of light
through a colloidal solutions appears strongly
luminous when viewed at right angle.
4. Brownian movement
- the suspended particles of colloidal
solutions are observed to be continuous, rapid
vibratory motion. This is attribute to the
incessant bombardment of the particles of the
solute by the movement of solvent.
5. Electrical charges
- colloidal particles especially suspensoids
are electrically charged, the charge being
distributed over the surface of the entire
particle. The migration of particles either to
positive and negative poles of an electricalfield is called ELECTROPHORESIS.
6. Surface tension
-the total surface area presented by colloidal
particles is enormous compared to those of
suspension.
Classification
a. Emulsoids
b. Suspensoids
Emulsoids
Are lyophillic, having affinity for the solvent.
Forming gel and swell when contact with large
Suspensoids
Are lyophobic ( no affinity for the solvent).
Exhibit Imbibition and Syneresis.
The colloidal state of suspensoids is rendered
1. Size of
particles
1 milimicron or
less
1 to 100
milimicron
Above 100
milimicra to 1
mm
2.Diffusibility
and filtrability
Passes thru
membrane &
filters
Passes thru
filters but not
thru
membranes
Do not pass
thru either
3. Visibility
Not visible
Visible under
Visible with
ultramicroscope microscope or
naked eyed
4. Motion
Not visible
Brownian
movement
Settles down
5.Osmotic
pressure
High
Low
None
6.Tyndal
none
exhibits
none
a. Surface Tension
b. Adsorption
-accumulation of substances of the surface of
solid or liquid.
- the greater the surface of the adsorbing
agent the greater is the adsorption. It is
increased by a rise of pressure and diminished
by a rise in temperature.
-the process of adsorption brings substances
nearer each other, thus promoting chemical
reactions.
Chromatography
Is a technique used to separate and identify
the components of a mixture of substances.
Methods:
1. Adsorption chromatography
5. Paper Chromatography
- one of the most ingenious methods of
utilizing partition chromatography is the
employment of paper as supporting medium.
6. Thin Layer Chromatography
7. Gel Filtration Chromatography
8. Gas Liquid Chrmatography
Emulsions
A dispersion of small drops of one liquid in
another liquid.
Types :
1.Emulsions in which oil is dispersed in water
known as the oil water type.
2. Emulsion in which water is dispersed in oil
known as the water oil type.
Viscosity
Liquid tends to flow due to its fluidity. The
resistance which a liquid offers to flowing is
viscosity. This varies greatly with different
liquids. The rate flow of a protein solution
through capillary tube by gravity or under
pressure is measured and compared with that
of the solvent alone.
Factors:
1. Temperature
2. Chemical nature
3. Colloids
Osmosis
Whenever two solutions of unequal
Diffusion
Is the interpenetration of molecules between
Dialysis
When two different solution are separated by