Diagnostic tests

Ainur Abdrakhmanova
 Diagnostic tests
CBC (leukopenia, thrombocytopenia in
50%)
Chemistry panel: liver and kidney function
tests
ESR: inflammation marker, used to monitor
Urinalysis: presence of protein,
wbc, rbc, cellular casts
BUN/creatinine: high
Complement: low
CRP: inflammation marker
 Anti phospholipid Ab: in 60%
Anti - Ro(SSA) and Anti La(SSB): against RNA
proteins, in 35-40% and 15% respectively
Anti-Sm Ab: in 25-30%, specificity is 99%
Anti-dsDNA: in 25-85%, specificity is almost
100%, titers correlate with disease activity

DNA binding and C3 94% (predictive value)

X - ray: abnormal shadows (fluid, inflammation

in the lungs)
Echocardiogram: real time image of beating
heart
Kidney biopsy
 ANA test
Sensitivity: 93-95%
Specificity: 57%
Healthy people positive low titer
Titer not used to assess the disease
activity
 ANA test

Immune ELISA Immunoblots

fluorescence (IF)
1. Pattern of
separation of
fluorescence cytoplasmic
generic Ag and Nu Ag by
2. Substrate used
3. ANA titer (1:160 specific MW
- In and
3% >)false positive
similar to IF multiple Ag transfer to
- Some patterns less on the strip/membran
defined plate e
- Difficult to specify Ab - Highly sensitive Incubation
- Used mainly for - False positive results with control
screening - Screening and and pt. serum
diagnosis
+ if band

- Only linear epitopes can be

detected
ANA pattern
 speckled homogeneous

Rim (peripheral) nucleolar

 Reference
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