CYTOMORPHOLOGY OF CANINE

TRANSMISSIBLE VENERAL TUMORS

Sudha Rani Chowdary. CH*, P.Vidya Sagar, M.

Raghunath
B. Sailaja and P. Ravi Kumar

Department of Teaching Veterinary Clinical Complex,

NTR college of Veterinary Science,
Gannavaram, Andhra Pradesh.
 Introduction

TVT -Transmissible venereal tumour (TVT)

also known as
Infectious sarcoma
Veneral granuloma
Transmissible lymphosarcoma
Sticker tumour

(GOLDSCHMIDT and HENDRICK, 2002).

 It mainly affects the

external genitalia
occasionally the internal genitalia
less commonly, lips or the other
portions of the skin or mucosa that
come in contact with the genitalia

(GOLDSCHMIDT and HENDRICK, 2002).

 OBJECTIVES

The incidence of TVT in canines that

were brought to TVCC, NTR CVSc,
Gannavaram and

Cytomorphology in genital and

extragenital TVTs.
 Incidence

20 cases of TVTs were recorded during the

period from March, 2013 to July, 2014.

Accounted for 38.5% of all the canine

neoplasms.

17 cases were recorded in bitches and 3 in

male dogs.
 External genitalia was the primary site in all the 20
dogs. In addition to the genital tumor, extragenital TVT
was identified in the oral cavity attached to the gum in
a bitch.

A circumscribed, A smooth, soft, small mass

elliptical, soft growth attached to the gingiva in
attached to the vulval the oral cavity of 0.5-1cm
lips (2-3 cm in diameter). diameter.
 Cytology

Impression smears of all the tumor

masses were collected and stained
with Leishmans stain.
Cytology revealed sheets of round cells with
cytoplasmic basophilia, intracytoplasmic
vacuolation, round to oval nuclei with coarse
chromatin and mitotic figures.

Photomicrograph showing sheets of Photomicrograph showing sheets of

TVT cells with cytoplasmic TVT cells with cytoplasmic basophilia
basophilia and intracytoplasmic and intracytoplasmic vacuolation and a
vacuolation (arrow). Leishmans mitotic figure (arrow). Leishmans
stain, 1000X stain, 1000X
 Cytomorphology- classification
100 TVT cells were counted on each slide to
determine the predominant cell type.

Lymphocytic type: predominance of 60%

or more of TVT cells with round morphology
and thin rim of cytoplasm.

Plasmacytic type: predominance of 60%

or more of TVT cells with ovoid morphology,
abundant cytoplasm and eccentric nucleus.
(Amaral et al; 2007)
 Cytology of genital TVTs
In all the genital TVTs, the cells were
lymphocytic type.

Photomicrograph showing sheets of

TVT cells with round morphology,
increased N:C ratio and thin rim of
cytoplasm.
Leishmans stain, 1000X
 Cytology of extra genital
TVT
In case of extragenital TVT, the cells were
plasmacytic type.

Photomicrograph showing
sheets of TVT cells with ovoid
morphology, abundant
cytoplasm, eccentric nucleus
and a mitotic figure (arrow).
Leishmans stain, 1000X
Comparison of genital and extragenital
TVT

Cytology of genital TVT showing Cytology of extragenital TVT

increased N:C ratio. showing cells with abundant
Photomicrograph depicts cells cytoplasm and eccentric nucleus.
with thin rim of cytoplasm. A mitotic figure is seen in the
photomicrograph.

LYMPHOCYTIC TYPE PLASMACYTIC TYPE

 Conclusions
Impression cytology is a reliable method for
diagnosis of suspected TVT.

TVTs of plasmacytic morphology presented higher

frequency of nuclear abnormalities suggestive of more
aggressiveness associated with metastasis
compared to that of lymphocytic type.

Hence, this type of classification would help

clinician to decide treatment modality.
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