M odifying H IV

Ashley Mattingly
 H ow to m odify H IV so it can be used to
regenerate cells in a blind eye.

Lab safety
Maneuvering around the lab
Prepping HIV for modification process
Modification and Cloning of the HIV process
Lab Safety

HIV is dangerous.
Wear gloves and lab coat
Make sure any openings in the skin are covered.
 M aneuvering around the lab

Syringe

Centrifug
e PCR Machine Lab oven and
Machine Refrigerator
Ice
Bucket
 Prepping the H IV Virus

Gather ice, 6 ml syringe, empty vial, a vial labeled HIV, and

a vial labeled plasma.
Put all labeled vials in the ice bucket.
Measure 1 ml of plasma and 3 mls of HIV.
Place the new vial in Centrifuge.
Leave for 6 hours.
Put all materials away.
M odifi
cation/Cloning Process

Gather primers 1-9

6 microliters of each primer
Micropipette (like a syringe but
Dial/Volum
e Adjuster with a dial and disposable tip)
Place in HIV/Plasma vial
Put in PCR machine

Removable Run 66 minutes

Tip
Put materials away
REFEREN CES

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surfaces as a tool for the surveillance of biosafety standards.Journal Of
Applied Microbiology,111(1), 70-82. doi:10.1111/j.1365-2672.2011.05042.x
Chulay, J. D. (2015, June). Retinal gene therapy moving closer to clinical
reality.Ophthalmology Times Europe. pp. 37-40.
Colella, P., Trapani, I., Cesi, G., Sommella, A., Manfredi, A., Puppo, A., & ...
Auricchio, A.
(2014). Efficient gene delivery to the cone-enriched pig retina by dual AAV
vectors.Gene Therapy,21(4), 450-456. doi:10.1038/gt.2014.8
Dalkara, D., Goureau, O., Marazova, K., & Sahel, J. (2016). Let There Be Light:
Gene and Cell