TintoStainer Training

Training for the 36, 48, and 72 Slide Automated TintoStainer

Bio SB Inc, 69 Santa Felicia Drive,

Santa Barbara, CA 93117
Overview

General Information about TintoStainer

Capabilities
Epitope Retrieval Options
Reagents
Competing Instruments

Operating TintoStainer
Programming TintoStainer
Protocol Programming
Reagent Programming
Manual
Overview (Part 2)

TintoStainer Financing
Instrument Purchase
Instrument Purchase (12 Month
Financing)
Reagent Acquisition (36 60 months)

TintoStainer/Bio SB Positioning
How to position the TintoStainer/Bio SB
Product lines to end users

Service
General Information
What is the TintoStainer?
Capabilities
Epitope Retrieval Options
Reagents
Competing Instruments
 What is the TintoStainer?

Automated IHC/ICC Instrument for use in Anatomic

Pathology laboratories

Used by both research and clinical labs.

Available in 36, 48, and 72 Slide configurations

Right: 36 Slide
TIntoStainer (BSB 0000)
loaded with barcoded
patient samples,
antibodies, and
detection systems.
What is the TintoStainer?

Uses unique
barcoding
technology.

Open systemuse
the reagents from
any supplier.

Comes bundled
with Bio SB Above: Barcoded reagent loaded into Bio SB TintoStainer.
The TintoStainer is an open platform, allowing user to
installation, training program in reagents from any supplier
and support
 What is the TintoStainer?
TintoStainer Components

A. TintoStainer Unit (36, 48,

or 72 Slide System)
B. Reagent Barcode
Printer
C. CPU Interface
D. Slide Barcode Printer
 Epitope Retrieval Options
TintoRetriever Pressure Cooker
(BSB 0000)

Fast Epitope Retrieval.

Cost Effective.
Uses very little reagent.
Built in temperature gauge
ensures proper
temperatures reached.
Bundled with
Immuno/DNA Retriever
Citrate & EDTA
Temperature Validation
Slides (Pictured)
Epitope Retrieval Options
TintoRetriever PT Module
(BSB 0000)
Touch interface allows user
to program exact time to
perform epitope retrieval
Integrates with TintoStainer
PC module.
Generates report of epitope
retrieval.
Reagents
Reagents
Which Reagents can be used in a TintoStainer?
Any reagent for ANY Supplier can be programmed into
the TintoStainer (Detection Systems, Chromogens &
Antibodies).
Completely open system that does not lock you to any
Bio SB reagent, ancillary or antibody.
Reagents
Comes pre-programmed with all Bio SB Reagents
Innovative line of Rabbit Monoclonal Antibodies for IVD use
Detection Systems
Bio SB ImmunoDetector HRP & AP
Bio SB PolyDetector HRP & AP
Bio SB PolyDetector PLUS HRP
 Reagents
Substrate Chromogens
HRP Based Chromogens
DAB
AEC
HRP Green
HRP Black
AP Based Chromogens
Alk-Magenta
Alk-Blue (Ready-to-use)
Alk-Brown (Ready-to-use)
Alk-Red (Ready-to-use)
TintoStainer Programming
Terminology (Reference figure below)
Reagent
Antibody
Protocol
Protocol Template
Refer to Page 8 & 9 in Manual
TintoStainer Programming

Selecting a protocol, Protocol

Template Option

TintoStainer comes pre-programmed

with all Bio SB protocols.

Protocol can also be built from

scratch.

Refer to Page 19 & 20 in Manual.

TintoStainer Programming
Programming Methodology
(Reference figure below)

Fast Programming Methodology

Patient Case Programming
Methodology
Refer to Page 10-12 on Manual
Reagent Programming
Bio SB TintoStainer
comes programmed
with all Rabbit & Mouse
Monoclonal/Polyclonal
Tinto Prediluted
antibodies

3rd part reagents can

easily be programmed
in.

Lots and Incubation

times are easily
changed

Refer to Page 23-28 in

manual
Additional Resources

TintoStainer Binder
Includes more in-depth information about TintoStainer
Includes setup/training outlines
TintoStainer Accessory list

TintoStainer Manual
In-Depth guide on programming reagents, customizing
instrument drop zones, etc.
Bio SB
info@biosb.com
Phone: +1-805-692-2768
Competing AutoStainers
TintoStainer vs. Competition

Bio SB TintoStainer Strengths BioCare Intellipath FLX Weaknesses

Lower Instrument Cost Higher Instrument Cost

Lower Reagent Cost (open) Higher Reagent Cost
Established reliability and Only partially accepted in field
acceptance
TintoStainer vs. Competition

Bio SB TintoStainer Strengths DAKO Link

Lower Instrument Cost Higher Instrument Cost

Lower Reagent Cost (open) Higher Reagent Cost (Closed)
Established reliability and Older instruments not serviced
acceptance
TintoStainer vs. Competition

Bio SB TintoStainer Strengths Ventana Benchmark

Leica Bond

Lower Instrument Cost Higher Instrument Cost

Lower Reagent Cost (open) Higher Reagent Cost (Closed)
Complete control of protocol Less operational flexibility
TintoStainer Financing
 TintoStainer Financing

Multiple Options for acquiring TintoStainer

Reagent Acquisition (w/ 36 - 60 month agreement)
Reagent commitment
Instrument Purchase
No reagent commitment
Instrument Purchase (w/ 12 month Financing)
No reagent commitment
Reagent Acquisition

Finance the instrument through the purchase of Bio SB

Reagents
Includes Bio SB Detection Systems, Ancillaries, and Tinto
Prediluted Antibodies.
Reagent Acquisition

How does a reagent acquisition work?

1. Contact Bio SB
2. Give Us the following information
a. Number of IHC Slides per Year
b. Length of Agreement
c. What TintoStainer(s) you are interested in acquiring
d. Epitope retrieval system requested
e. Preferred detection chemistry
3. We provide a monthly commitment amount & Cost per slide.
4. You keep the instrument!
Reagent Acquisition

We will then reply back with

a. Requested down payment (to
cover part of equipment cost).
b. Monthly financial commitment to
Bio SB for reagent.
c. Annual amount of detection
system that is included in
agreement.
d. Annual amount of antibody
included in agreement.
e. Cost per test.
Reagent Acquisition Form
Reagent Acquisition Example
Scenario 1 (Small Volume)
User performs 5000 IHC Tests Annually.
3 year period.
Wants 36 Slide Instrument
Wants to enroll in a reagent rental
agreement that includes every reagent
necessary.
Wants to use the New Bio SB PolyDetector
PLUS HRP Detection with Bio SB Rabbit
Monoclonal Antibodies.
How much will it cost this person per slide
and per month?
Reagent Acquisition Example

Bio SB Provided Equipment

1 TintoStainer 36 Instrument
1 TintoRetriever Pressure Cooker

Bio SB Provided Reagents

Ancillaries (ImmunoRetriever, ImmunoWasher,
etc.)
Bio SB Tinto Predilutes
Bio SB Detection System (Block, Link, Label)
Bio SB Substrate Chromogen of choice
(Choose from all 8!)
Reagent Acquisition Example

Down Payment/Security Deposit

$15,000

Monthly Commitment
$3,721.61 Per Month

Cost Per Slide

$9.27 Per Slide
Reagent Acquisition Example
Scenario 2 (Medium Volume)
User performs 10,000 IHC Tests Annually.
3 year period.
Wants 48 Slide Instrument
Wants to enroll in a reagent rental
agreement that includes every reagent
necessary.
Wants to use the New Bio SB PolyDetector
PLUS HRP Detection with Bio SB Rabbit
Monoclonal Antibodies.
How much will it cost this person per slide
and per month?
Reagent Acquisition Example

Bio SB Provided Equipment

1 TintoStainer 48 Instrument
1 TintoRetriever Pressure Cooker

Bio SB Provided Reagents

Ancillaires (ImmunoRetriever,
Immunowasher, etc.)
Bio SB Tinto Predilutes
Bio SB Detection Chemistry of your choice
Bio SB Substrate Chromogen of choice
(Choose from all 8!)
Reagent Acquisition Example

Down Payment/Security Deposit

$15,000

Monthly Commitment
$5, 985.50

Cost Per Slide

$7.18 Per Slide
Reagent Acquisition Example
Scenario 3 (Large Volume)
User performs 15,000 IHC Tests Annually.
5 year period.
Wants a 48 Slide Instrument
Wants to enroll in a reagent rental
agreement that includes every reagent
necessary.
Wants to use the New Bio SB PolyDetector
PLUS HRP Detection with Bio SB Rabbit
Monoclonal Antibodies.
How much will it cost this person per slide
and per month?
Reagent Acquisition Example

Bio SB Provided Equipment

1 TintoStainer 48 Instrument
1 TintoRetriever Pressure Cooker

Bio SB Provided Reagents

Ancillaires (ImmunoRetriever,
Immunowasher, etc.)
Bio SB Tinto Predilutes
Bio SB Detection Chemistry of your choice
Bio SB Substrate Chromogen of choice
(Choose from all 8!)
Reagent Acquisition Example

Down Payment/Security Deposit

$15,000

Monthly Commitment
$5, 707.77

Cost Per Slide

$4.57 Per Slide
TintoStainer 36 Financing

Instrument Purchase with Financing

End User pays Bio SB down
payment, with rest financed for 1
year through Bio SB
Down Payment = $15,000
15% APR

Price - $4,312.50 per month

TintoStainer 36 Financing

Instrument Purchase (36 Slide)

End User pays Bio SB for entire cost
of instrument
Competitively priced unit
Still includes training, setup and
support for 1 year
Includes preventative maintenance
after 6 month period

Price - $60,000
Bio SB Product Positioning

How do Bio SB Antibodies and reagents differ from the

competition?
Competitively priced
FDA/cGMP certified
Largest array of IVD Rabbit Monoclonals (130+)
Large array of innovative detection systems
Bio SB Antibody Pricing

On average, when
comparing prices of Bio SB
vs. other brands, Bio SB is
cheaper by about
$35 per mL!
Talking Points

Important talking points when position Bio SB reagents

Bio SB portfolio of Rabbit Monoclonals (130+!) which have
higher sensitivity and specificity than mouse counterparts.
Probe with customers to see if they have any problem
antibodies
Does your customer have any backorder issues?
What is your cost per test for __________________
Unique antibodies/clones in Bio SB portfolio.
Declining reimbursement for diagnostic medical devices
(U.S.)
 Key Antibody Panels

Bio SB has a large portfolio of

IVD/ISO manufactured
antibodies.

Available in concentrate or
predilute format.

330+ Antibodies for use in

molecular pathology.

130+ Rabbit Monoclonal

antibodies.
Bio SB Reagents in Other Autostainers
Questions?

Thank you!