REPLIKASI

DNA

Dr. Oeke Yunita, S.Si., M.Si., Apt.

1
Synthesis Phase (S phase)
S phase during interphase of the
cell cycle
Nucleus of eukaryotes
S
DNA replication takes phase
place in the S phase.

G1 interphase G2

Mitosis
-prophase
-metaphase
-anaphase
-telophase
Handout Biologi Sel, Dr. Oeke Yunita 2
 DNA Replication

Begins at Origins of Replication

Two strands open forming Replication
Forks (Y-shaped region)
New strands grow at the forks
3

Parental DNA Molecule

5 Replication
Fork
3

Handout Biologi Sel, Dr. Oeke Yunita 3 5

 DNA replication system

Template: double stranded DNA

Substrate: dNTP
Primer: short RNA fragment with a
free 3-OH end
Enzyme: DNA-dependent DNA
polymerase (DDDP),
other enzymes,
protein factor
Handout Biologi Sel, Dr. Oeke Yunita 4
 Players involved in DNA Replication
Origin of Replication Enzymes (Initiator Proteins) recognize the start site on
template DNA and initially open up the DNA (single strands)
Helicases untwist the double helix at the replication forks
Primase begins DNA replication by laying an an RNA primer (10 nt long) for DNA
polymerase to start at
Single-strand binding proteins bind to and stabilize the single-stranded DNA
until it can be used as a template
DNA polymerase polymerizes new DNA in a 5 to 3 direction, proofreads the
newly laden DNA, excises error ridden DNA and replaces that DNA with the
correct sequence. Speed/fidelity: mutates 1 X 10-10
Topoisomerase corrects overwinding ahead of replication forks by breaking,
swiveling, and rejoining DNA strands
Terminal replication proteins stall replication fork progress for polymeriation
to catch-up
DNA ligase anneals nucleotides together, creating a DNA strand

Handout Biologi Sel, Dr. Oeke Yunita 5

 TAHAP TAHAP
REPLIKASI DNA
1. Denaturasi (Pemisahan) untaian DNA
template
2. Inisiasi
3. Pemanjangan untaian DNA
4. Ligasi fragmen DNA
5. Terminasi

Handout Biologi Sel, Dr. Oeke Yunita 6

 III III

I I

DNA

Handout Biologi Sel, Dr. Oeke Yunita 9

 Replication of Strands
Point of Origin
Replication
Fork

Handout Biologi Sel, Dr. Oeke Yunita 10