Electrical characterization of

nanowire bridges incorporating

biomolecular recognition elements

L u S h a n g , Ta m i L a s s e t e r C l a re , M a r k
A Eriksson et al.
Department of Chemistry and Physics, University
o f Wi s c o n s i n - M a d i s o n , M a d i s o n , U S A

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 Contents

1. Introduction
2. Experiment
3. Results
4. Conclusion
 1.Introduction

! Integration of biomolecular recognition with nanoscale

systems applications [biological sensing, control
nanoscale assembly]
!! Nanoscale materials manipulation Dielectrophoretic
manipulation (directs nanoscale object to specific place)
!!! Dielectrophoretic manipulation + electrical detection
methods directing temporarily individual bacterial cells
across electrode
 1.Introduction

Physical manipulation + biomolecular recognition

unique types of nano-bio-hybrid structures (by
ligating structures in place)
Formation and electrical
characterization of nanowire
bridges nanowires
modified with biotin span
(biomolecule) across a gap
between avidin (biomolecule) 3.5 m
functionalized gold
microelectrodes.
 1.Introduction

Nanowire bridges
Potential way to sense the presence of biomolecules

A biologically activated digital switch

Avidin (a tetrameric biotin

Biotin (vitamin B7 and formerly binding protein produced in
known as vitamin H or coenzyme R) the oviducts of birds, reptiles and amphibians
and deposited in the whites of their eggs)
 2. Experiment

Gold nanowires (Easy to synthesize and manipulate) preparation

1. Synthesized by electrochemical deposition in porous alumina membrane
109 nanowires simultaneously - 250 50 nm diameter and 60 m length
2. Sputter coating silver metal contact on one side of membrane
3. Electrochemical reduction from gold plating solution (j = 5 mA*cm-2) gold
nanowires deposited inside the pores (10 m length)
4. Removing the silver in nitric acid
5. Releasing the Au nanowires by dissolving the membrane in 6M NaOH
6. Rinsing several times Au nanowires in deionized water with centrifugation
7. Suspensing Au nanowires in absolute ethanol
 2. Experiment

Chemical functionalization
1. Immersing nanowires in 1 mM of 11amino undecanethiol hydrochloride
(MUAM) in ethanol
2. Shaking for 12 h to form amine-terminated thiolate
3. Exposed amine groups react 30 min with sulfosuccinimidyl
biotinamidohexanoate (SSBAH)
 2. Experiment

Gold microelectrodes
1. On oxidized silicon wafers by conventional UV
lithography and metal evaporation (teardrop shape = a
region of high electric field for nanowire)
2. The same steps for immobilizing the biotin into
electrodes before and after avidin saturation of them
Gold thin film
1. Functionalized with biotin (completely) and with avidin
(half) into a suspension of biotinylated nanowires for 30
min.