Prepared by:

Dagdagan, Jeanne Frances S.

 Question #1
• A sample of material from bluish purulent head
lesions is submitted for analysis. A gram negative,
motile, non-spore forming oxidase positive rod was
isolated. This organism is most likely:

a) Proteus mirabilis
b) Proteus vulgaris
c) Pseudomonas aeruginosa
d) Stenotrophomonas maltophilia
 Question #1
• A sample of material from bluish purulent head
lesions is submitted for analysis. A gram negative,
motile, non-spore forming oxidase positive rod was
isolated. This organism is most likely:

a) Proteus mirabilis
b) Proteus vulgaris
c) Pseudomonas aeruginosa
d) Stenotrophomonas maltophilia
Pseudomonas aeruginosa is the only oxidase positive organism listed.
 Question #2
• Organisms of this genus are gram negative, motile
rods. A few are chromogenic and produce a red
non-water soluble pigment . Some have been
implicated in septicemia, pulmonary and urinary
tract infections. One member of this genus is

a) Pseudomonas aeruginosa
b) Sarcina lutea
c) Serratia marcescens
d) Proteus mirabilis
 Question #2
• Organisms of this genus are gram negative, motile
rods. A few are chromogenic and produce a red
non-water soluble pigment . Some have been
implicated in septicemia, pulmonary and urinary
tract infections. One member of this genus is

a) Pseudomonas aeruginosa
b) Sarcina lutea
c) Serratia marcescens
d) Proteus mirabilis
The only organism listed which produces a red pigment is Serratia.
Options B and D are cocci.
 Question #3
• Which characteristic is most useful in
differentiating Citrobacter and Salmonella?

a) H2S production
b) Indole production
c) Lysine decarboxylase
d) Urease production
 Question #3
• Which characteristic is most useful in
differentiating Citrobacter and Salmonella?

a) H2S production
b) Indole production
c) Lysine decarboxylase
d) Urease production

The classic biochemical reaction which separates these two genera is

lysine. The other biochemical reactions can be variable.
 Question #4
• A gram negative rod, isolated from the urine of a
female with recurrent UTI, was oxidase negative,
urease positive showing A/A with H2S on TSI is most
likely

a) Escerichia coli
b) Klebsiella pneumonia
c) Proteus mirabilis
d) Pseudomonas aeruginosa
 Question #4
• A gram negative rod, isolated from the urine of a
female with recurrent UTI, was oxidase negative,
urease positive showing A/A with H2S on TSI is most
likely

a) Escerichia coli
b) Klebsiella pneumonia
c) Proteus mirabilis
d) Pseudomonas aeruginosa

Options A and B are H2S negative and deaminase negative. Option D

is K/K on TSI and oxidase positive.
 Question #5
• An organism recovered from a diarrheal stool was
K/A with no gas or H2S on TSI, lysine negative,
oxidase negative, urease negative and citrate
negative. This organism is most likely:

a) Aeromonas hydrophila
b) Escherichia coli
c) Proteus vulgaris
d) Shigella sonnei
 Question #5
• An organism recovered from a diarrheal stool was
K/A with no gas or H2S on TSI, lysine negative,
oxidase negative, urease negative and citrate
negative. This organism is most likely:

a) Aeromonas hydrophila
b) Escherichia coli
c) Proteus vulgaris
d) Shigella sonnei

Option A is oxidase positive. Option B is A/A on TSI and indole positive.

Option D is H2S positive and urease positive.
References:
• Harr, R. R. (2013). Medical laboratory science
review (4th ed.). Philadelphia: F.A. Davis
• Bishop, M. L., Fody, E. P., & Schoeff, L. E.
(2013). Clinical chemistry: principles, techniques,
and correlations. 7th ed. Philadelphia: Wolters
Kluwer Health/Hippincott Williams & Wilkins.
• Hubbard, J. D. (2009). A Concise Review of Clinical
Laboratory Science. 2nd ed. Philadelphia: Hippincott
Williams & Wilkins.