Chromatography

Chemistry
6R
Boys group 2
Presentation
Group members

 The team members of our team : Umer (captain)

Krishna
Naqi
Ramithu
Jabez
Rohail
Harsh
Trashid
 What is chromatography

 Chromotography is a physical method of separating mixtures.

Chromatography is used to separate mixtures of colored compounds .
 The various constituents of the mixture travel at different speeds, causing them
to separate.

Mikhail Tswet Super fact

Mikhail Tsvet invented
chromatography in 1900
during his research on plant
pigments. He was a Russian
botanist
Types of chromatography

 There are many chromatography’s

paper chromatography
Thin-layer chromatography.
Gas chromatography.
etc.…

Super fact
Mikhail twestt died in the
age of 47
Paper chromatography and
uses
Uses of paper chromatography
Paper chromatography

1. It is used separate colored chemicals or

 Paper chromatography is an analytical substances
method used to separate colored
chemicals or substances. It is primarily
used as a teaching tool, having been 2. It is used in the sequencing of DNA and RNA.
replaced by other chromatography
methods, such as thin-layer
chromatography.
Steps in paper chromatography

 Step 1: Gather the Needed Materials (coffee Filter, Pencil,

Beaker, Water Soluble Pen/marker, Water)
 Step 2: Cut a Strip of Filter Paper From the Coffee Filter and
Using the Marker Draw a Thick Line Near the Bottom of the
Filter Paper- About 1cm From the Bottom.
 Step 3: Allow the Water to Move Up the Paper for Approx. 5
Minutes and Then Remove the Strip From the Water
 Step 4: You can identify the ink changed into several colors
 Thin layer chromatography

Uses of chromatography
Thin layer chromatography
 Thin-layer chromatography
(TLC) is a chromatography
 chromatography in which technique used to separate
compounds are separated on non-volatile mixtures
a thin layer of adsorbent
material, typically a coating of
silica gel on a glass plate or
plastic sheet.
Gas chromatography

Gas chromatography Uses of gas chromatography

Gas chromatography is a common type used in analytical chemistry for separating

of chromatography used in analytical and analyzing compounds that can be
chemistry for separating and analyzing vaporized without decomposition
compounds that can be vaporized
without decomposition
Things we should not do in
chromatography

 There are many ways or methods to do chromatography so there many things we should
not do in common chromatography
in paper chromatography we should not keep on adding water
in thin layer chromatography we should not keep it uneven.

Super fact
Did you know we should
be accurate to make
that the test becomes
perfect
Lets watch an video