An Ayurvedic Formulation of a Cream for

Treating Rheumatoid and Osteoarthritis

Binoy K. Bordoloi, Ph.D., MBA

Director, Bordoloi Biotech India Pvt. Ltd.(BBIPL)
CEO, BORDOLOI BIOTECH, LLC (usa)

Guwahati – December 8, 2017

Jorhat – December 24, 2017
A Unique Natural Formulation For Freedom From Joint Pain
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Bordoloi Biotech India Pvt Ltd. (BBIPL)

Binoy (Ben) K. Bordoloi, Director

- Ph.D. (NYU)
- MBA(UCLA)
- Chief Scientific Officer
- IP and Legal Liaison
- BusinessDevelopment
- Past experience at Baxter, Johnson & Johnson

Kulwant S. Saini, Director

- M.Pharma
- Ph.D.
- Chief of Regulatory Affairs & Quality Assurance
- Clinical Affairs
- Past experience at Johnson & Johnson

Manash P.Baruah, Director

- Licensee of a French Diagnostics Company
- Head, Manufacturing Operations of BBIPL
- Head, Marketing Operations of BBIPL
- Head, e-Commerce of BBIPL
 PROMOTERS’ VIEWS ON THE PRODUCT
Dr. BINOY B. BORDOLOI
“Demonstrated efficacy of reduction of inflammatory cytokines with the synergistic blend of
essential oils was established in an arthritic animal model with topical treatment. It was carried
out in the labs of Prof. Samir Bhattacharya (Former Director IICB - Kolkata, CSIR-NEIST -
Jorhat, and Research Emeritus Professor, School of Life Sciences, Visva-Bharati, a Central
University, Santiniketan, West Bengal)”. (Please see next 7 slides.)

Dr. KULWANT S. SAINI

“HerboJoint is a combination of Mother Nature’s time tested herbs into a scientific, evidence
based product meeting the needs of patients for alleviating crippling joint pain thereby
improving day-to-day quality of life.”
KATTRNA (Cymbopogon citratus) OIL
Cymbopogon citratus, commonly known as lemon grass or oil grass, is a tropical plant from
South Asia and Southeast Asia.

Laboratory studies have shown cytoprotective, antioxidant, and anti-inflammatory properties in

in vitro studies.

Research has demonstrated that the plants essential oil enhances GABA-ergic neurotransmission
at sufficient doses (with an anxiolytic threshold dose of 10 mg/kg) via positive allosteric
agonism in the same manner as benzodiazepines (eg. diazepam) which are used clinically
as anxiolytics, sedative/hypnotics, muscle relaxants....
Cymbopogon Citratus Oil (Lemongrass Oil) is very well known in India. It
has been studied extensively in Japan as well (Mitoshi et al , “Suppression
of allergic and inflammatory responses by essential oils derived from herbal
plants and citrus fruits”, International Journal of Molecular Medicine, 33:
1643 – 1651, 2014). They extensively investigated 20 essential oils, and
reported that Lemongrass Oil was the most efficacious of them all with
respect to in-vitro anti-allergic and anti-inflammatory activities. (Please see
the Chart in next slide.)
SATI (Hedychium spicatum) OIL
Known as Sati in Ayurveda, Kappor Katcheri herb and the essential oil extracted from the
rhizomes of this plant through steam distillation process has been in use traditionally for treating
inflammation and certain other problems...

Kappor Katcheri is a Hindi name and is scientifically called as Hedychium spicatum. It is a limb of
the Ginger family Zingiberaceae. It is a small hardy ginger with bright and large white and
orange flowers and is indigenous to the subtropical Himalayan regions in India at an altitude of
1000 to 3000 m...
TUMURU (Zanthoxylum armatum) OIL
With the botanical name Zanthoxylum armatum, the Winged Prickly Ash tree is a member of the
Citrus family Rutaceae. Tomar tree is indigenous to Eastern Asia to China along the Himalayas
and few other parts of India.

This oil was trusted to give warmness to the body and was recommended for treating ailments
like rheumatism...
MITOSHI et. Al.: THE BIOLOGICAL ACTIVITY OF ESSENTIAL OIL FROM LEMONGRASS

Figure 1 - In vitro anti-allergic and anti-inflammatory

activities of essential oils (EOs) isolated derived from

20 plant species. (A) Inhibition of β-hexosaminidase

release in RBL-2H3 cells treated with the calcium

ionophore, A23187. (B) Inhibition of tumor necrosis

factor-α (TNF-α) production by RAW264.7 cells

treated with lipopolysaccharide (LPS). EOs, 100

μg/ml; Data are shown as the means ± standard

deviation (SD) of 5 independent experiments.

**P<0.01 and *P<0.05 vs. controls (untreated cells).

THREE ESSENTIAL OILS
The three essential oils have proven efficacy for pain relief in the joints. Based on published data
in scientific literature in India, Japan and other countries these herbal oils are reported to be
effective in Rheumatoid arthritis and Osteoarthritis.

Pharmacological trials established that systemic manifestation of Rheumatoid arthritis may be

accounted for by release of inflammatory molecules of Cytokines from joints such as TNF-alfa
(tumour nercosis factor), IL-1beta, and 1L-6 in arthritic animal model the oil blend was found to
bring down/regulate these arthritic markers in blood serum in pre and post measurement of
these Cytokines.

1) B. K. Bordoloi and K. S. Saini, Indian Patent Application No.: 201631031742 dated September 17,
2016, “Anti-inflammatory Activity with Synergism of Herbal Essential Oils”. (This demonstrates the
synergistic efficacy of Cymbopogon citratus, Zanthoxylum armatum and Hedychium spicatum oils).
2) M. Mitoshi, et al, International Journal of Molecular Medicine 33: 1643-1651, 2014, “Suppressionof
Allergic and Inflammatory Responses by Essential Oils Derived from Herbal Plants and Citrus Fruits”.
(It discloses the highest efficacy of Cymbopogon citratus out of 20 essential oils).
3) D. Giri et al, Journal of Medicinal Plants Research, Vol 4(25), pp 2773-2777, 2010; it discloses the
anti-inflammatory activity of Hedychium spicatum oil.
4) Thokchom et al, Indian Journal of Natural Products and Resources, Vol 2(3), September 2011, pp
275-285 (Phytochemical and pharmacological profile of Zanthoxylum armatum DC).
 Collagen Induced Arthritis (CIA)
Collagen type II (3mg/ml) has been
dissolved in 10 mM Acetic Acid

1ml Complete Freund’s Adjuvant

+
1 ml CII solution

Immunization of mice
Inject i.d. 50 ml /mice weighing 20-25 gm

1st Immunization – Day 1

2nd Immunization – Day 21 NATURE PROTOCOLS, 2007
 After 4 days of 2nd immunization
(Day 25)

Visible changes:
Slow movement Serum parameters:
Loss of cage riding ability
Redness of paw ELISA- TNFa, IL1b, IL6

Arthritic mice treated with different combinations of

Oils (LG, ZA, HS) for 21 days (n=6)
Fig-1A: ELISA Inflammatory Cytokines Study on Arthritic Mice Model : TNF-alpha

TNF-alpha
160

140

120
pg/mg Protein

100

80

60

40

20

0
Control CIA CIA+CC+ZA CIA+CC+HS CIA+CC+ZA+HS

CIA = Collagen Induced Mice; CC = Cymbopogon citratus oil;

ZA = Zanthoxylum armatum oil; HS = Hedychium spicatum oil
Fig-1B: ELISA Inflammatory Cytokines Study on Arthritic Mice Model : IL-6

IL-6

400

350

300
pg/mg Protein

250

200

150

100

50

0
Control CIA CIA+CC+ZA CIA+CC+HS CIA+CC+ZA+HS

CIA = Collagen Induced Mice; CC = Cymbopogon citratus oil;

ZA = Zanthoxylum armatum oil; HS = Hedychium spicatum oil
Fig-1C: ELISA Inflammatory Cytokines Study on Arthritic Mice Model : IL-1beta

IL-1beta
120

100

80
pg/mg Protein

60

40

20

0
Control CIA CIA+CC+ZA CIA+CC+HS CIA+CC+ZA+HS

CIA = Collagen Induced Mice; CC = Cymbopogon citratus oil;

ZA = Zanthoxylum armatum oil; HS = Hedychium spicatum oil
 Collagen Induced Arthritic (CIA) Mice:
CIA Mice After Treatment : Climbing
Before Treatment: Dragging Feet to Walk
 THE PRODUCT
HERBOJOINT, a unique composition of three effective oils for joint pain relief.

Composition:
 Kattrna (Cymbopogon citratus) oil 3.0%
 Sati (Hedychium spicatum) oil 1.0%
 Tumuru (Zanthoxylum armatum) oil 1.0%
Cream base q.s

*Besides providing relief from joint pain it works effectively in Osteoarthritis and Rheumatoid
Arthritis
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Blood Plasma Analysis of the Inflammatory Markers in the Arthritic Animal Model after
Three Weeks of Topical Treatment
Results are from the Labs of Prof. Samir Bhattacharya, Visva-Bharati, Santiniketan
Patient Safety and Efficacy Clinical
Trials were Carried out at Govt.
Ayurvedic College and Hospital
Guwahati, Assam

Dr. B P Sarma and Dr. K Jolly

Efficacy of Joint Pain Reduction
in 40 patients in 4 Weeks
Efficacy of Swelling Reduction
in 17 patients in 4 Weeks.
Efficacy of Joint Stiffness Reduction
in 20 patients in 4 Weeks
 DOSAGE & APPLICATION

Apply three times a day for 3 weeks on clean and unbroken skin of
the affected joint areas.

Apply approx..1-4 cm (0.5 - 2.0 gm) expressed length of HERBOJOINT 3 times daily
over the clean unbroken skin of affected joints for a period of at least 3 weeks and
beyond. The dose varies (1 cm or 0.5 gm for fingers; 2 cm or 1.0 gm for areas other
than knee; and 3-4 cm or 1.5 to 2.0 gm for knees) depending on the surface area of
the affected joint and the intensity of the pain. A 75gm tube may last for 7-10 days.
 INDICATION

Joint Pain
………….arising due to

Osteoarthritis

Rheumatoid Arthritis
 SUPREMACY
• HerboJoint contains Mother Nature’s time tested herbs (rather than chemical and
NSAIDs etc.) in a synergistic combination
• HerboJoint is formulated into an aqueous base (oil in water) cream which is easy
for lavigation and dissipates rapidly into the skin for easy deposition of the
essential oils on to the skin for absorption and action.
• HerboJoint is in Non greasy (no oily feel) and non-staining base.
• It is effective and has no side effects at all.
PACK SIZE AND PRICING

Pack size: Each Tube Contains 75g of Cream

Price Structure: Maximum Retail Price Rs.279/-

 BORDOLOI BIOTECH INDIA PVT. LTD.

OUR ESTABLISHMENTS

 Head Office: A-20 Okhla Industrial Area, Phase-I, New Delhi-110020

 Regional/Sales Office: 4 Dr. Suresh Sarkar Road, RTC House, Kolkata-700014

 Manufacturing Unit: MS LAB, EPIP Complex, Amingaon, Guwahati-781031

Joint pain… Not again

Thank You….