Class: 6-2

Student:
● Chicaiza Rodríguez Mishel
 OBJECTIVES

• Obtain nanoparticles from the coffee seed extract and

the addition of 0.001 M of ferric chloride to synthesize
and obtain zero iron Valente under atmospheric
conditions.
• Show a test of organic compounds from
phytochemical screening
• Perform a UV analysis to find the diameter of the
nanoparticles and the change in absorbance.
RESUM

In the present work, the green synthesis of nanoparticles

to obtain zero iron Valente using the aqueous extract of
seeds of the robust coffee plant has been reported.
Phytochemical tests were carried out to make their
respective test of organic compounds and determine the
presence of resins, saponins, reducing compounds,
phenolic compounds, flavonoids and alkaloids. The
aqueous solution of ferric chloride was used, after
reacting with the coffee extract, led to the very stable and
crystal clear rapid formation of iron nanoparticles.
 Treatment of
contaminated
groundwater

Eliminate
contaminants Disinfection
relevant including APPLICATIONS byproducts
viruses and bacteria

Microbicide
 Obtaining extract
In a beaker place the coffee seed and wash with
distilled water, let the sample dry and then crush it in
a homemade blender. In a beaker place distilled
water and heat with 15g of the sample during 30
minutes in a range of 65-70 ° C with constant
agitation, let cool the sample. The extract is filtered
using filter paper, the filtrate is collected in a test
tube and placed in refrigeration.
 PHYTOCHEMICAL SCREENING
Phytochemical screening or phytochemical screening is one of the
initial stages of phytochemical research, which qualitatively
determines the main chemical groups present in a plant

Resin Test - - -
Reducing - (greenish blue) - (yellow) - (yellow)
compounds. Fehling
trial
Saponins Foam test - - -
Phenolic + test (dark green) + (dark green) + (dark green)
compounds. Ferric
chloride
Flavonoids + + +

Alkaloids +++ (Precipitated) +++ (Precipitated) ++ (Defined

Dragendorff Tests Turbidity)

Alkaloids Mayer Test +++ (Precipitated) ++ (Defined ++ (Defined

Turbidity) Turbidity)
 Reaction mechanism
Quinic acid, acid 1,3,4,5-tetrahidoxycyclohexane-1-carboxylic, is a
vegetable acid very abundant in nature that was found in the cinconarinda
and also in the coffee seeds and leaves of many plants
 Synthesis of zero-valence iron nanoparticles

During the synthesis of

nanoparticle iron both the
precursor and the reducing
agent were mixed in a
flask. For the reduction of
the Fe ions, the filtrate of
the seed extract was added
and 0.001 M of FeCl 3 was
mixed in an aqueous
solution with constant
stirring at a temperature.
Within a certain time it
turns from a Brown color
to a Brown color obtained
by nanoparticles.
 Conclusions
It has been shown that the
extract is very effective for
the preparation of iron
nanoparticles that shows
good stability in solution,
by virtue of the UV-Visible
wavelength, nanoparticles
show resonance behavior.
The ferric chloride with the
agent reduction the coffee
seed extract has shown a
remarkable color change
with the change in question
in the pH of the solution.
The success of such a rapid
time scale for synthesis of
metallic nanoparticles is an
alternative to chemical
synthesis protocols and low
cost reduction to synthesize
iron nanoparticles.