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relatively expensive,
e.g., rennin obtain from calf's stomach,
◦ the value depend on demand of lamb or beef,
◦ and their availability.
b) Plant sources
While some plant enzymes are relatively easy
to obtain
e.g., papain from papaya, bromelain from
pineapple, actinidin from kiwi fruit
◦ their supply is also governed by food demands
c) Microbial enzyme
Microbial enzymes are produced by methods
which can be scaled up easily
Recombinant DNA technology now provides
◦ α-amylase
◦ b-amylase
◦ Amyloglucosidase/glucoamylase
Starch contains straight-chain glucose
polymers called amylose and a branched
component known as amylopectin.
The branched structure is relative more
α-amylase
The action of α-amylase reduces the
to tree
Hydrolysis of cellulose
cellulase – enzyme that hydrolyze cellulose
◦ Trichoderma fungi are commonly used at the
present time.
◦ They are thoroughly developed and characterized at
present.
◦ There are three major classes of enzymes for
different substrates and products
1.Exo-b-1,4-cellobiohydrolase (CBH)
2.Endo-b-1,4-glucanase
3. b-glucosidase
Hydrolysis of cellulose
3 steps of reaction catalyze by cellulase :
1)Breakage of non-covalent interactions
present in the crystalline structure of
cellulose by endo-β-1,4-glucanase
noncollagen protein
Environmental friendly rather than using
chemical
4) Dairy industries
- Coagulation of milk
Rennin remove glucopeptide from soluble
precipitates
Esterase
Cleave or synthesize ester bonds to yield an
acid and an alcohol
lipase
of paper
Conventional method – used chemical to
enzyme preparations
eg. Blend of diff. amylase yields large amount of
cell walls.
Used as an antibacterial agent, treatment of
to aspartic acid
Some cancer cells require asparagine
allergic individuals
Convert the drug into nonallergic form
Thank You