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NANOPARTICLE

 A set of material substances which have at least one dimension


less than 100 nm

 Its surface layer will be functionalised with a variety of small


molecules, metal ions, surfactant and polymers for specific
applications
CELL SORTING
• The isolation of certain types of cells from
Definition mixed cell population of organs or tissues

Single-
Bulk cell-based
sorting sorting Cell separation
Separate large
that done
number of
through flow
desired cells
cytometry
ENZYMATIC TRANSFORMATION OF PHOSPHATE
DECORATED MAGNETIC NANOPARTICLES FOR
SELECTIVELY SORTING AND INHIBITING CANCER CELL

Nanoparticle: D-tyrosine Phosphate Decorated


Magnetic Iron Oxide Nanoparticles
Function: Separate Cocultured Cancer Cell from
Stromal cell
Method: Magnetic Separation
WHAT IS THE MECHANISM ??
Enzymatic
Transformation
Placental alkaline Dephosphorylate the Form tyrosine
phosphatase phosphate-bearing coated magnetic
(ALPP) magnetic nanoparticles
nanoparticles

ALPP
LAST STEP
SIMPLE AND EFFICIENT CAPTURE OF EGFR-EXPRESSING TUMOR
CELLS USING MAGNETIC NANOPARTICLES
Anti-epidermal growth factor receptor (EGFR)
Nanoparticle antibody-conjugated dimercaptosuccinic acid-coated
magnetic nanoparticles (DMSA-MNPs)

Function Separate EGFR-expressing PCG (lung


adenocarcinomas) from fetal bovine serum (FBS)

Polyethylene
glycol (PEG-) Reduce the non-specific binding of cells
linker

Method Immunomagnetic cell separation


Direct magnetic
labelling

Positive selection

WHY
USING THIS
METHOD ?
IMMUNOLOGICAL MECHANISM
RECEPTOR BLOCKAGE : To prevent the ligand-induced EGFR tyrosine
kinase activation

(epidermal growth EGFR


factor and
transforming
growth factor) anti- EGFR
antibody
EGFR-
expressing
PCG
PCG
cell

Red
blood
cell
IN SITU MAGNETIC SEPARATION AND
IMMOBILIZATION OF DIBENZOTHIOPHENE-
DESULFURIZING BACTERIA

Aims for high-level desulfurization


Conventional hydrodesulfurization (HDS) unit operate at severe
conditions- high temperature, high pressure, long residence time
There goes Biodesulfurization(BHS)
it is to use cell to desulfurize
Then, cells are immobilization and removed with magnet
Nanoparticle
• Oleate-modified Fe3O4

Function
• Separate Rhodococcus erythropolis from its
original culture fermentation broth
Method
• Magnetic separation
Adsorption mechanism is due nano-sized effect and
hydrophobic interaction between bacterial cell wall and
nanoparticles