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Single-
Bulk cell-based
sorting sorting Cell separation
Separate large
that done
number of
through flow
desired cells
cytometry
ENZYMATIC TRANSFORMATION OF PHOSPHATE
DECORATED MAGNETIC NANOPARTICLES FOR
SELECTIVELY SORTING AND INHIBITING CANCER CELL
ALPP
LAST STEP
SIMPLE AND EFFICIENT CAPTURE OF EGFR-EXPRESSING TUMOR
CELLS USING MAGNETIC NANOPARTICLES
Anti-epidermal growth factor receptor (EGFR)
Nanoparticle antibody-conjugated dimercaptosuccinic acid-coated
magnetic nanoparticles (DMSA-MNPs)
Polyethylene
glycol (PEG-) Reduce the non-specific binding of cells
linker
Positive selection
WHY
USING THIS
METHOD ?
IMMUNOLOGICAL MECHANISM
RECEPTOR BLOCKAGE : To prevent the ligand-induced EGFR tyrosine
kinase activation
Red
blood
cell
IN SITU MAGNETIC SEPARATION AND
IMMOBILIZATION OF DIBENZOTHIOPHENE-
DESULFURIZING BACTERIA
Function
• Separate Rhodococcus erythropolis from its
original culture fermentation broth
Method
• Magnetic separation
Adsorption mechanism is due nano-sized effect and
hydrophobic interaction between bacterial cell wall and
nanoparticles