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CHAPTER 1:
INTRODUCTION OF ANALYTICAL CHEMISTRY
SYLLABUS CONTENT
Types and steps in analysis.
Review the terms: moles, molarity and concentration.
Other expressions of concentration: % (w/w), % (w/v),
% (v/v), ppm, ppt, ppb.
Calculations involving different expressions of concentrations.
LEARNING OUTCOME
• Chemical analysis:
Determination of the chemical composition or chemical
make up as well as the quantity of each composition
presence in a sample.
Examples:
o The chemical analysis of a blood sample involves the determination of
its iron content, its alcohol content, or perhaps its drug content and
how much each of the composition presence in the blood sample.
QUALITATIVE QUANTITATIVE
ANALYSIS ANALYSIS
Analysis to identify the Analysis to determine
constituents (elements, the relative amounts of
atoms, ions, compounds) the constituents
present in a sample. presence in a sample.
QUALITATIVE QUANTITATIVE
To determine the PRESENCE or ABSENCE of a To determine the EXACT AMOUNT of that
substance in a sample substance/analyte in a sample
CHEMICAL IDENTITY of the substance of the QUANTITY of the species in a sample.
sample. (Concentration/level/percentage/mass/relative
(Type of species/analyte/atom/ion/ abundance)
molecule/functional group present)
Eg: Eg:
1. The presence of copper ion detected 1. The concentrations of oxygen in blood
when flame colour is green samples is equal to 3 mL O2/liter blood.
2. Identifying anions and cations in 2. The percentage by mass of silver is 42.88% in
aqueous solution ore.
Eliminating interferences
Gross sample:
• consist several portions of tested material.
• must be reduced in size to gain laboratory sample.
Laboratory sample:
• small portion, crushing or sieving to form
homogeneous or uniform powder for analysis.
• the analysis sample that actually analyzed.
STEP 4 PREPARE THE SAMPLE FOR ANALYSIS
INTERFERENCE
Species other than analyte
that can cause ERROR & affect
the final measurement
STEP 6 PERFORMING THE MEASUREMENTS
Replicates samples
Improve the quality of the results & provide a measure
reliability.
Analysis
Incorporates the measurement of the concentration of the
analyte in replicates and comparing with standards.
Gravimetric analysis
based on MEASUREMENT OF MASS (precipitation)
Volumetric analysis
based on MEASUREMENT OF VOLUME (titration)
STEP 6 PERFORMING THE MEASUREMENTS
Instrumental techniques
MOLE
• The mole (mole) is the SI unit for the amount of a chemical
species.
• One mole (mol) is the amount of a substance that contains as
many entities (atoms, molecules or ions) as there are in 12.0 g
of C12 atoms.
• The mass in grams of 1 mole of a substance
is called its molar mass.
2. MOLES, MOLARITY, CONCENTRATION
RELATIONSHIP BETWEEN MOLE & NUMBER OF
ENTITIES
For example:
1 mol of Mg atom contain 6.022 x 1023 Mg atoms.
1 mol of H2O molecule contain 6.022 x 1023 H2O
molecules.
1 mol of Na+ ions contain 6.022 x 1023 Na+ ions
EXERCISES
1 g= 1000 mg
1 mol = 1000 mmol
1 L = 1000 mL
EXAMPLE
EXAMPLE
EXAMPLE
FORMULA
EXERCISES
RELATIONSHIP BETWEEN MOLE & NUMBER OF ENTITIES
1. Calculate the number of moles for 3.4 g of carbon atoms.
(0.28 moles)
2. Find the mass of 0.30 moles of CH4 (4.8 g)
3. Calculate no of moles of Cl- is present in 0.34 moles of CaCl2
(0.68 moles)
4. Find the mass of H in 2.5 moles of H2O? (5.0 g)
5. Find the mass of O in 3.0 moles of CO2? (96.0 g)
2. MOLES, MOLARITY, CONCENTRATION
CONCENTRATION
WATER is considered a
universal solvent for
most solutions
1 L = 1000 mL
Remember!! 1 dm3 = 1000 cm3
• convert volume
to L or dm3
2. MOLES, MOLARITY, CONCENTRATION
MOLALITY
Number of moles of solute that dissolved in 1000 g of solvent, m
Preferred expression of concentration involving colligative
properties such as (boiling point elevation, freezing point
depression, osmotic pressure)
Unit for molality, m = (mol/kg)
FORMULA!!
m = no of mol of solute (mol)
1 kg of solvent (kg)
EXAMPLE
Next, convert the volume of solvent to the weight of solvent using the
density
= 1.00 L x 0.779 kg/L = 0.779 kg
Mass of solvent:
1 L of solution = 1000 mL = 1000 cm3
1.329 g/cm3 times 1000 cm3 = 1329 g (the mass of the entire solution)
1329 g - 571.4 g = 757.6 g = 0.7576 kg (the mass of water in the solution)
% CONCENTRATION
1. % WEIGHT/WEIGHT
% (w/w)
EXAMPLE
Calculate the molarity of the following acids.
1. % WEIGHT/WEIGHT
% (w/w)
EXAMPLE
c) Briefly explain how to prepare the following solution in the laboratory. 500 mL of 2.0 M
H2SO4 from a commercial reagent at 90 % (w/w) purity and specific gravity of 1.84.
So, measure and transfer 59.2 mL from commercial reagent to 500 mL of volumetric flask.
Dilute with distilled water until calibration mark. Shake upside down.
3. EXPRESSION OF CONCENTRATION (PERCENTAGES %)
2. % WEIGHT/VOLUME TO INDICATE THE
2. % WEIGHT/VOLUME
% (w/v)
EXAMPLE
1. Briefly explain how to prepare the following solutions ( 1.0 L, 10.50 % (w/v) aqueous
CH3CH2CH2OH.
Weigh 105 g of CH3CH2CH2OH, dissolve with small amount of distilled water, transfer to 1.0 L of
volumetric flask, dilute with distilled water to the calibration mark. Shake upside down.
3. EXPRESSION OF CONCENTRATION (PERCENTAGES %)
Part per thousand (ppt), part per million (ppm) and part per billion (ppb)
For sample that contain smaller amount of component the concentration is
expressed in terms of ppt, ppm or ppb.
EXAMPLE
1. Calculate w/v in ppm of sodium in a solution containing
2.500mg of Na+ in 500 mL of solution.
Or
? g x 109 = 1.20
103 mL
? g = 1.2 x 103
109
= 1.2 x 10-6 g
= 1.2 µg
EXAMPLE
3. Determine the ppm of ferrous ion, Fe2+, in a solution known
to be 1.2 × 10-6 M Fe3(PO4)2.8H2O
(RMM=501.61) Answer: 0.6ppm
EXAMPLE
4. Describe the preparation of 1 L 0.1 M HCl from
concentration HCl 37% w/w and with density 1.18 g/mL
EXAMPLE
1. Molarity (concentrated) = percentage (%) x (density/RMM) X 1000
2. M1V1 (concentrated) = M2V2 (diluted)
Using 2 formula:
EXAMPLE
EXAMPLE
5. Describe the preparation of a 250 mL 50 ppm solution
of AgNO3 from solidchemical.
EXAMPLE
6. Describe the preparation of 0.5 L 50 ppm of Cl- from solid KCl.
EXAMPLE
7. Calculate mass in gram of Ba(NO3)2 is needed to prepare a 50 mL
solution containing 100 ppm NO3-?
EXAMPLE
EXAMPLE
EXAMPLE
EXAMPLE
How many milliliters of concentrated sulfuric acid, 94.0% (g/100 g
solution), density 1.831 g/cm3, are required to prepare 1 L of 0.100
M solution?
Extra note!
Preparation of solution: Describe to prepare the solution - include 2 parts
1) calculation and
2) sentence to prepare the solution (in detail)
• Transfer to volumetric
flask (mL)
• Measure mL • Dilute until calibration
mark.
• Shake upside down
EXERCISES
1. Define:
a) Parts per million
b) Molality
c) Molarity
d) Percentage