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• Staphylococcus saprophyticus:
important cause of UTI in young
women
• Novobiocin: RESISTANT
Family Streptococcaceae
• Streptococci
• Gram-positive cocci occurring in pairs or in chains
• Nonmotile
• Non-spore forming
• Facultative anaerobes
• Catalase negative
Classification
• Lancefield Classification
• By antigenic character of a group-specific cell wall polysaccharide
• Almost 20 Lancefield groups are identified
• Brown’s Classification
• Classified according to the pattern of hemolysis
Hemolysis Species
Alpha Streptococci pyogenes,
Viridans strep. And some
Group D.
Beta Streptococci pyogenes and
Lancefield Classification
Group Species
A Group A Streptococci
(Streptococci pyogenes)
B Group B Streptococci
(Streptococci agalactiae)
C Streptococci equisimilis
Streptococci zooepidemicus
Streptococci equi
Streptococci dysagalactiae
D Entrococci
Entrococci faecalis
Entrococci faecium
Entrococci durans
Entrococci avium
Non-Entrococci
Streptococcus bovis
Streptococcus equinus
Brown’s Classification
Hemolysis Species
Alpha Streptococci pyogenes, Viridans strep.
And some Group D.
NEUFELD QUELLUNG
11. Francis test
• Detection of pneumococcal antibodies
Gram Positive Bacilli
BACILLUS
CLOSTRIDIUM
CORYNEBACTERIUM
LISTERIA
ERYSIPELOTHRIX
LACTOBACILLUS
KURTHIA AND ROTHIA
Bacillus
• Gram positive bacilli that form spores AEROBICALLY.
• Catalase POSITIVE
Bacillus anthracis
• is the most virulent, and the agent of ANTHRAX
• * Cutaneous anthrax = malignant pustule (black eschar)
• * Woolsorter’s disease or pulmonary anthrax
• * Gastrointestinal anthrax
• Bacillus anthracis is the largest bacteria, largest pathologic bacilli
and it is NON-MOTILE.
Laboratory Diganosis
COLONIES LIFTED BY LOOP OR
NEEDLE
MEDUSA HEAD COLONIES “BEATEN EGG CONSISTENCY”
Laboratory Diagnosis
FROM CULTURE BAMBOO
FROM PATIENT CAPSULATED POLE ARRANGEMENT
Laboratory Diagnosis
STRING OF PEARLS
(MHA WITH PENICILLIN)
Bacillus cereus
• Is associated with food poisoning isolated from several foods: rice,
other cereals, vegetables and milk.
• “FRIED RICE POISONING” = vomiting and diarrhea
• MOTILE
Bacillus anthracis vs Bacillus cereus
CCFA MORPHOLGY
Biochemical test for CLOSTRIDIA SPP.