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the Encoding of
Biological
Information
Chapter 3
Griffith’s Experiment
Virulent Nonvirulent
bacteria bacteria
Avery,
Macleod, and
Nonvirulent
bacteria
McCarty’s
Experiment
Virulent and
nonvirulent Nonvirulent bacteria only
bacteria
Protein
mRNA
Ribosome
Translation
Phosphate
group
Deoxyribose
sugar
The Four Bases
5′-AGCT-3′
Phosphodiester
bond
3'-hydroxyl
group
3' 5'
Sugar–
phosphate
backbone
34 Å =
3.4 nm
(10 base
pairs) per Major groove
complete
turn
Minor groove
3' 5'
Diameter 20 Å = 2 nm T A G C
Hydrogen Bonding
A and T are held together by
two hydrogen bonds.
Sugar–
Hydrogen phosphate
bonding backbone
DNA Replication
Parental strands serve
as the templates for
the daughter strands.
Parental Daughter
strands strands
6
Condensed chromatid Chromatin
1400 nm in diameter
5
Coiled coil
4 700 nm in
Coiled diameter
chromatin fiber
300 nm in
diameter 3
Chromatin
fiber 30 nm
2 in diameter
Nucleosome Histone
proteins 1
fiber 10 nm in DNA duplex 2
diameter nm in diameter
Nucleosome
DNA vs. RNA
Ribose Deoxyribose
Uracil Thymine
(U) (T)
Ribose Deoxyibose
DNA vs. RNA
DNA RNA
Sugar Deoxyribose Ribose
Bases A, T, C, G A, U, C, G
5′ end Monophosphate Triphosphate
Size Very large Smaller
Strands Double Single
Template vs. Nontemplate Strands
Template strand
RNA transcript
Nontemplate strand
Initiation and Termination of Transcription
Transcription is initiated at a promoter
sequence and ends at a terminator
sequence. The transcript is synthesized
in a 5'-to-3' direction.
TATA
TATA
Promoter region
RNA Polymerase II Adds Nucleotides
RNA
transcript
RNA
polymerase
complex (Pol II)
Template RNA–DNA duplex
DNA strand
Polymerization Reaction
Template strand
1
Incoming ribonucleotides
RNA transcript are accepted if they
correctly base pair with
the template DNA.
Direction of 2
The 3'–OH of the
chain growth
growing strand
attacks the high-
energy phosphate
bond of the
incoming
ribonucleotide,
providing the energy
to drive the reaction.
Hydrogen 3
bonding The two phosphates of
the incoming
ribonucleotide are
released as
pyrophosphate.
RNA Polymerase in Prokaryotes
RNA transcript
RNA polymerase
acts here
Incoming
RNA
DNA already
trinucleotides
transcribed
DNA still to
Nontemplate be
DNA strand transcribed