The Effects on the Rise Time of Filter Paper from the

Concentration of H2O 2

William Henderson, Jayla Bush, Jacob Alexander, and Luke Cogdell

Sandy Spring Friends School 16923 Norwood Rd, Sandy Spring, MD 20860

Introduction: Results: F.g.7 Results Graph

Conclusions:
Enzymes are proteins that act as a biological F.g.1. Enzyme ● As expected the mean increased and decreased as a
catalyst, speeding up chemical reactions in result to the amount of H2O2.
cells. The enzyme we used in this experiment ● The speed of the paper decreased when testing with
was catalase (chicken liver solution). more hydrogen peroxide.
The research objective th ● Variation of 3% or less did not generate a
F.g.4.
the question of, Substrate statistically significant result.
“What are the effects ● We found that our alternative hypothesis was
of rise time/reaction of correct and that when the hydrogen peroxide was
the filtered paper from F.g.2. F.g.3. O2
exposed to more H2O2, the filter paper rose at a
the different H2O
quicker/faster pace.
concentrations of
H2O2?” The effects This diagram shows the
Future Directions:
enzymes and substrate
were determined through
before and after the
different concentrations of enzymatic action Methods: 2
H2O2 (hydrogen peroxide) ● Complete more trials for further comparison.
that would affect the speed of the filtered paper, that had previously ● First we placed the filter paper into ● Use more and different concentrations for H2O2
been soaked in the catalase. Our alternative hypothesis was that the the 25mL of chicken liver solution (catalase) (highest was 3%).
the filtered paper would reach the top of the hydrogen peroxide faster or 20 seconds of soakage. ● Use a different type of liver (a different animal).
when presented with more H2O2. Through our methods, we were able ● Next we removed the filter paper
to conduct and find the answers to our research question. from the chicken liver solution (catalase) and
placed it into the H2O. Acknowledgments:
F.g.6. ● We then waited for the reaction to occur
Picture
(timed the reaction from first contact of filter This research was supported by Angela Colegrove and
paper, to solution)(measured time to the the SSFS Science Department.
hundredths place).