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(GAS CHROMATOGRAPHY)
Angela Wulansari
Introduction • Chromatography
– Chromatography is a general term applied to a
wide variety of separation techniques based
on the portioning or distribution of a sample
between a moving or mobile phase and a fixed
or stationary phase (Nielsen, 2010).
• Gas Chromatography
– Gas chromatography is the separation
technique that is based on the multiplicative
distribution of the compounds to be separated
between the two phase system solid or liquid
(stationery phase) and gas (mobile phase)
(Gunzler and Williams, 2002)
Advantage • Requires only very small samples with
little preparation
of GC • Good at separating complex mixtures
into components
• Results are rapidly obtained (1 to 100
minutes)
• Very high precision
• Only instrument with the sensitivity to
detect volatile organic mixtures of low
concentrations
• Equipment is not very complex
(sophisticated oven)
The GC System
1. Gas supplies
2. Sample injector
3. Column
4. Detector
5. Oven
6. Data station
7. Recorder
The GC
Theory
The GC • Mobile phase : Gas
Theory Gas Liquid • Stationary phase: liquid
Chromatography • Principle : volatility,
partition