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ORF 2 (5147-7126)
5` NTR
(1-27)
HVR Signal
ORF1 encodes a protein of 1693 peptide 3` NTR
aminoacids containing functional motifs
include methyl transferase, protease, RNA (7127-94)
helicase, and RNA-dependent RNA ORF2 encodes the viral capsid protein of 660
aminoacids responsible for virion assembly,
polymerase. interaction with target cells and immuno
genicity.
Epidemiology
• Genotype 1 : Asia, Africa
• Genotype 2: Mexico, Africa
• Genotype 3: World wide
• Genotype 4: South East Asia
• HEV genotype 1 is more conserved and five subtypes: 1a, 1b, 1c, 1d,
and 1e.
• Genotype 2 two subtypes: 2a and 2b.
Also high seroprevalence among blood donors in south west France and
South West England
• Arankalle and Choube from Pune, India, have shown 1.5% (3/2
of blood donors to be positive for HEV RNA and suggested the
possibility of transmission by transfusion.
Study from developed countries
• Cons: Expensive, labour intensive, special training for working in molecular Lab.
What should be testing Strategy
HEV in India
• Q2: Should we go for alternative testing?
developing countries, a simple and non laborious test such as
V-Ag, enabling early detection of viral infection prior to the
currence of IgM antibodies, would enhance the management
HEV infection. [Detection of HEV antigen as a novel marke
r the diagnosis of hepatitis E. J. Med. Virol. 2006; 78:1
448.]