Multilocation Test Some Melon Genotype

(Cucumis melo L. var. Makuwa) in

Three Regions

Diah Rusita Handayani

155040201111279

SUPERVISED BY
Prof. Ir. Sumeru Ashari, M. Agr. Sc. Ph. D

AGRICULTURE FACULTY OF BRAWIJAYA UNIVERSITY

Melon (Cucumis melo L.) merupakan salah satu komoditas buah-
buahan yang banyak digemari oleh masyarakat karena memiliki rasa
yang manis dan warna daging buah yang bervariasi.
Mulai tahun 2014 produksi buah melon mengalami penurunan. Salah
satu permasalahan yang dihadapi petani melon adalah masalah benih.
Usaha untuk meningkatkan produksi buah melon dapat ditempuh
melalui intensifikasi, diantaranya dengan cara memperbaiki teknologi
budidaya.
Salah satu cara yang bisa dilakukan adalah menggunakan bibit unggul
yang mempunyai kualitas lebih baik.
Agar varietas unggul dapat stabil diberbagai kondisi lingkungan, maka
perlu dilakukan beberapa tahapan pengujian varietas.Tahapan tersebut
diantaranya uji multilokasi yang bertujuan untuk mengetahui daya hasil
dan pertumbuhan calon varietas.
Melon (Cucumis melo L.) is one of the fruit commodities that is much
favored by the community because it has a sweet taste and the color of
fruit flesh varies.
Starting in 2014 the production of melon fruit has decreased. One of
the problems faced by melon farmers is the problem of seeds.
Effort to increase melon production can be achieved through
intensification, including by improving cultivation technology. One
way that can be done is to use superior seeds that have better quality.
In order for high-yielding varieties to be stable in various
environmental conditions, it is necessary to do several stages of
varieties testing. The stage include multilocation tests which aim to
determine the yield and growth potential of the varieties.
 Introduction
Background
Melon (Cucumis melo L.) is one of the fruit commodities that is
much favored by the community because it has a sweet taste
and the color of fruit flesh varies.

Starting in 2014 the production of melon fruit has decreased.

One of the problems faced by melon farmers is the problem of
seeds.

Efforts to increase melon production can be achieved through

intensification, including by improving cultivation technology.
One way that can be done is to use superior seeds that have
better quality.

In order for high-yielding varieties to be stable in various

environmental conditions, it is necessary to do several stages of
varieties testing. The stages include multilocation tests which
aim to determine the yield and growth potential of the varieties.

Background
Melon (Cucumis melo L.) is one
of the fruit commodities that is
much favored by the community
because it has a sweet taste and
the color of fruit flesh varies.
Efforts to increase melon production
can be achieved through
intensification, including by
improving cultivation technology.
One way that can be done is to use
superior seeds that have better
quality.
AGRICULTURE FACULTY OF BRAWIJAYA UNIVERSITY
Introduction
Starting in 2014 the production of
melon fruit has decreased. One of
the problems faced by melon
farmers is the problem of seeds.
In order for high-yielding varieties to be
stable in various environmental
conditions, it is necessary to do several
stages of varieties testing. The stages
include multilocation tests which aim to
determine the yield and growth
potential of the varieties.
 Introduction

This study aims to determine which genotypes are

superior to other tested genotypes and to determine the
Purpose effect of genotype x environment interactions on
agronomic characters grown in Malang Regency,
Ponorogo Regency and Batu City.

The hypothesis proposed in this study is that there are

genotypes that have advantages over other tested ones
Hypothesis
and there are effects of genotype x environment
interactions on agronomic characters grown in Malang
Regency, Ponorogo Regency and Batu City.

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 Materials and Methods

Place and Time

1. Ngijo Village, Karangploso District, Malang Regency
2. Sooko Village, Sooko District, Ponorogo Regency
3. Pendem Village, Junrejo District, Batu City

Desember 2018 – March 2019

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 Materials and Methods

Tools Materials

1. Hoe 1. BME 4140

2. Meter 2. BME 2800
3. Analytical scales 3. BME 2802
4. Refractometer 4. Varieties Silver light
5. RHS colour chart 5. NPK Fertilizer
6. Tweezers 6. KNO3 Fertilizer
7. Sprayer 7. Multi KP Fertilizer
8. Marker 8. Pesticide
9. Calipers 9. Mulch (MPHP)
10. Stationery 10. Stake
11. Camera

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 Research Method

The research method that used in each experimental location is

Randomized Complete Block Design (RCBD) with single factor
that is genotype. The experiment will be repeated 3 times. So there
are 12 experimental plots in each location.

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 Implementation of Research

Land processing Germination Seeding

Weed control and

Fertilization Planting
disease pests

Harvest

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 Variable Observations
Qualitative
Quantitative

1. Stem shape
2. Stem colour 1. Size of leaf length and width
3. Leaf shape 2. Size of fruit length and width
4. Leaf colour 3. Thick fruit flesh
5. Flower shape 4. Fruit cavity width
6. Flower petals colour 5. Sweetness level
7. Flower crown colour 6. Fruit weight per plot
8. The color of stigma 7. Fruit weight
9. Stamen colour 8. Fruit yield per hectare
10. Fruit shape 9. Flowering age
11. Fruit skin colour 10. Harvest age
12. The colour of fruit flesh
13. The taste of fruit flesh
14. Seed shape
15. Seed colour

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 Data Analysis

The data analyzed by using variance (ANOVA). Then Analysis

of Variance (ANOVA) was collected in three locations in one
season. If there are significant differences, then proceed with
the HSD (honestly significance diffirence) test with a level of
5%.

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 Sketch of Experiment

Location Batu Location Malang and Ponorogo

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 Sketch of Experiment

X X X X X X X X X X X X X X X

X X X X X X X X X X X X X X X

Note:
1. Plant spacing 50 x 60 cm
2. Plot width 8 m
3. Plot length 1,1 m
4. X is sample plants

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 Qualitatif Character
Result

Genotype Stem shape Stem colour

BME 2802 Round Green

(YGG RHS 144A)
STEM

BME 4140 Round Green

(YGG RHS 144A)
BME 2800 Round Green
(YGG RHS 144A)
Silver Light Round Green
(YGG RHS 144A)

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 Qualitatif Character
Result

Genotype Leaf Shape Leaf Colour

BME 2802 Pentalobate Dark green

(GG RHS N137A)
LEAF

BME 4140 Pentalobate Dark green

(GG RHS N137A)
BME 2800 Pentalobate Dark green
(GG RHS N137A)
Silver Light Pentalobate Dark green
(GG RHS N137A)

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 Qualitatif Character
Result

Flower Flower petals Flower crown Stigma Stamen

Genotype Flower shape
colour colour colour colour colour

Yellow Yellowish Yellow Yellowish Yellow

(YG RHS 9B) Green (Y RHS 12A) Green (Y RHS
BME 2802
FLOWER

Rotate
(YG RHS 144B) (YG RHS 12A)
144 A)
Yellow Yellowish Yellow Yellowish Yellow
(YG RHS 9B) Green (Y RHS 12A) Green (Y RHS
BME 4140 Rotate
(YG RHS 144B) (YG RHS 12A)
144 A)
Yellow Yellowish Yellow Yellowish Yellow
(YG RHS 9B) Green (Y RHS 12A) Green (Y RHS
BME 2800 Rotate
(YG RHS 144B) (YG RHS 12A)
144 A)
Yellow Yellowish Yellow Yellowish Yellow
(YG RHS 9B) Green (Y RHS 12A) Green (Y RHS
Silver Light Rotate
(YG RHS 144B) (YG RHS 12A)
144 A)

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 Qualitatif Character
Result

Fruit skin Fruit flesh Taste of fruit

Genotype Fruit shape
colour colour flesh

White White
BME 2802 (GWG RHS (WG RHS NN Fresh sweetness Round
FRUIT

157 D) 155B)
White White
BME 4140 (GWG RHS (WG RHS NN Fresh sweetness Oblate
157 D) 155B)
White White
BME 2800 (GWG RHS (WG RHS NN Fresh sweetness Oblate
157 D) 155B)
White
White
(WG RHS NN
Silver Light (GWG RHS 155A) Fresh sweetness Round
157 C)

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 Qualitatif Character
Result

Genotype Seed colour Seed shape

BME 2802 Cream Roundish

(YG RHS 162 A)
SEED

BME 4140 Cream Roundish

(YG RHS 162 A)
BME 2800 Cream Roundish
(YG RHS 162 A)

Silver Light Cream Roundish

(YG RHS 162 A)

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 Quantitatif Character
Result

Combined Analysis

Interaction
Character Genotype Location
GxL

Leaf length (cm) 0.71 tn 1.58 tn 0,28 tn

Leaf width (cm) 0.23 tn 2.22 ** 0.20 tn
Fruit length (cm) 7.27 ** 2.23 ** 0.15 tn
Fruit width (cm) 1.13 ** 2.20 ** 0.12 tn
Fruit weight (gram) 4806.77 ** 33344.11 ** 1054.30 tn

472.60 **
Fruit weight per plot (kg) 281.41 ** 14.69 **

Fruit cavity width (cm) 2.07 ** 0.25 tn 0.18 tn

Thick fruit flesh (cm) 0.24 ** 0.25 ** 0.01 **
Sweetness level (Brix) 7.42 ** 0.10 tn 1.00 **
Fruit yield per ha (ton ha-1) 144.74 ** 86.30 ** 4.50 **
Flowering age (DAP) 3.66 ** 4.08 tn 0.60tn
Harvest age (DAP) 110.62 ** 79.08 ** 2.01 **

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 Quantitatif Character
Result

Location
Genotype
Malang Batu Ponorogo
Leaf Length

BME 2802 16.33 16.32 16.64

BME 4140 16.24 15.75 16.07
BME 2800 15.58 15.50 16.37
Silver Light 15.83 15.18 16.56
HSD 5% tn
Average 16.00 15.69 16.41
Note: Numbers followed by the same lowercase letter in one column show no significant difference in the HSD test level of
5%.

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 Quantitatif Character
Result

Genotype Leaf width (cm)

BME 2802 15.63

Leaf Width

BME 4140 15.03

BME 2800 15.10

Silver Light 15.03

Average in Malang 14.89 a
Average in Batu 14.86 a
Average in Ponorogo 15.62 b
HSD 5% Location 0.54
Note: Numbers followed by the same lowercase letter in one column show no significant difference in the HSD test level of
5%.

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 Quantitatif Character
Result

Genotype Fruit length (cm)

BME 2802 8.50 b

BME 4140 9.82 d

Fruit Length

BME 2800 9.13 c

Silver Light 7.71 a

HSD 5% Genotype 0.10

Average in Malang 9.22 c

Average in Batu 8.35 a

Average in Ponorogo 8.80 b

HSD 5% Location 0.03

Note: Numbers followed by the same lowercase letter in one column show no significant difference in the HSD test level of
5%.

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 Quantitatif Character
Result

Genotype Fruit width (cm)

BME 2802 8.44 c

BME 4140 7.87 a

Fruit Width

BME 2800 7.67 a

Silver Light 8.27 b

HSD 5% Genotype 0.11

Average in Malang 8.33 b

Average in Batu 7.57 a

Average in Ponorogo 8.29 b

HSD 5% Location 0.08

Note: Numbers followed by the same lowercase letter in one column show no significant difference in the HSD test level of
5%.

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 Quantitatif Character
Result

Genotype Fruit weight (gram)

BME 2802 375.33 c

Fruit Weight

BME 4140 358.44 bc

BME 2800 320.22 a

Silver Light 354.56 b

HSD 5% Genotype 12.36

Average in Malang 390.75 c

Average in Batu 292.08 a

Average in Ponorogo 373.56 b

HSD 5% Location 14.64

Note: Numbers followed by the same lowercase letter in one column show no significant difference in the HSD test level of
5%.

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Fruit Weight Per Plot (kg) Quantitatif Character
Result

Location
Genotype
Malang Batu Ponorogo

BME 2802 31.03 f 19.30 cd 25.10 e

BME 4140 21.97 d 12.13 b 18.67 cd

BME 2800 20.57 d 7.73 ab 17.57 c

Silver Light 9.63 b 5.67 a 7.33 ab

HSD 5% Interaction 2.86

Average 20.80 11.21 17.17

Note: Numbers followed by the same lowercase letter in one column show no significant difference in the HSD test level of
5%.

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 Quantitatif Character
Result

Genotype Fruit cavity width (cm)

Fruit Cavity Width

BME 2802 5.24 c

BME 4140 4.67 a

BME 2800 4.84 b

Silver Light 5.74 d

HSD 5% Genotype 0.12

Average in Malang 5.24

Average in Batu 4.96

Average in Ponorogo 5.16

Note: Numbers followed by the same lowercase letter in one column show no significant difference in the HSD test level of
5%.

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 Quantitatif Character
Result

Location
Thick Fruit Flesh (cm)

Genotype
Malang Batu Ponorogo

BME 2802 1.64 f 1.37 c 1.79 g

BME 4140 1.70 f 1.45 d 1.66 f

BME 2800 1.50 de 1.23 ab 1.53 e

Silver Light 1.34 bc 1.17 a 1.28 b

HSD 5% Interaction 0.06

Average 1.54 1.31 1.57

Note: Numbers followed by the same lowercase letter in one column show no significant difference in the HSD test level of
5%.

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 Quantitatif Character
Result

Location
Genotype
Sweetness Level (Brix)

Malang Batu Ponorogo

BME 2802 8.31 de 8.04 d 8.79 e

BME 4140 7.13 c 7.57 cd 6.17 b

BME 2800 5.68 a 6.69 bc 6.28 b

Silver Light 7.81 d 7.13 c 7.49 cd

HSD 5% Interaction 0.55

Average 7.23 7.36 7.18

Note: Numbers followed by the same lowercase letter in one column show no significant difference in the HSD test level of
5%.

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 Quantitatif Character
Result

Location
Results Per Ha (ton ha -1)

Genotype
Malang Batu Ponorogo

BME 2802 17.18 f 10.68 cd 13.89 e

BME 4140 12.16 d 6.72 b 10.34 cd

BME 2800 11.39 d 4.28 ab 9.73 c

Silver Light 5.34 b 3.14 a 4.06 ab

HSD 5% Interaction 1.59

Average 11.52 6.20 9.50

Note: Numbers followed by the same lowercase letter in one column show no significant difference in the HSD test level of
5%.

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 Quantitatif Character
Result

Location Flowering age (DAP)

Flowering age (DAP)

BME 2802 22.44 b

BME 4140 21.67 a

BME 2800 23.22 c

Silver Light 22.33 b

HSD 5% Genotype 0.34

Average in Malang 22.67

Average in Batu 22.83

Average in Ponorogo 21.75

Note: Numbers followed by the same lowercase letter in one column show no significant difference in the HSD test level of
5%.

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 Quantitatif Character
Result

Location
Harvest Age (DAP)

Genotype
Malang Batu Ponorogo

BME 2802 56.67 ab 58.00 d 56.33 ab

BME 4140 56.33 ab 57.00 bcd 55.33 a

BME 2800 57.00 bcd 57.33 bcd 55.67 ab

Silver Light 64.33 e 64.00 e 63.33 e

HSD 5% Interaction 1.08

Average 58.92 59.83 55.00

Note: Numbers followed by the same lowercase letter in one column show no significant difference in the HSD test level of
5%.

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 Conclusion

Based on the results of the study it can be concluded as follows:

1. The BME 2802 genotype is the genotype that shows the best compared to BME
4140 and BME 2800 genotypes in the three test locations seen from the character
of productivity.
2. Characteristics of fruit weight per plot, fruit flesh thickness, sweetness level (Brix),
fruit yield per hectare and harvest age showed significant differences in genotype
interactions with the environment.

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 Documentation

Location Malang Location Batu

Location Ponorogo

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 Documentation

Leaf color

Flower
color

Stem color

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 Documentation

Thick fruit flesh

Fruit skin color

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 THANK YOU

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