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Synthesis
Function of DNA as “master” program
• DNA codes for the primary structure of a protein which impacts the
tertiary structure, which determines the function of the protein
• DNA doesn’t control protein synthesis directly…instead
DNA VS RNA
Differences Between DNA and RNA
DNA RNA
double-stranded single-stranded
• Occurs in 3 stages
Transcribe the following DNA:
Transcription – 3 Stages
1. Initiation
- RNA polymerase binds to the DNA at the
promoter region. It is directed to this
region by proteins called initiation factors.
2. Elongation
- RNA polymerase moves along the coding
strand of DNA away from the promoter,
building the RNA strand.
3. Termination
- RNA polymerase reaches the terminator
region of the DNA & is released from the DNA
with the primary transcript.
RNA Processing
• All 3 types of RNA (mRNA, tRNA, &
rRNA) are processed in the nucleus
before they leave it.
• This can involve adding, chemically
changing, and/or removing
nucleotides. (proofreading)
RNA Splicing
All RNA transcripts undergo processing called splicing.
Practice:
tRNA:
tRNA and Amino Acids
-There are 64 possible
codon combinations (3
nitrogen bases) BUT
only 20 amino acids.
- SO – some codon
“words” code for the
same amino acid.
- We can interpret or
“read” this code using a
codon table:
Let’s try “AUG”
Now try:
• CCU
• CCG
• UGA
• GGA