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Amino acids
Amino group
(NH2)
Carboxyl group From: Elliott, WH. Elliott, DC. (1997) Biochemistry
(COOH)
and Molecular Biology. Oxford: Oxford University
Press. p23
Amino acids
20 amino acids make
up protein
8 essential amino
acids
From: Elliott, WH. Elliott, DC. (1997) Biochemistry
9 in infant (histidine)
Dipolar
and Molecular Biology. Oxford: Oxford University
Press. p23
+ve end (NH3+)
-ve end (COO-)
Protein structure - bonding
Disulfide bond
Ionic bond
Hydrophobic force
Peptide bond
Peptide bond joins
amino acids
Bond at both ends
Increases range of
possible proteins
2 peptides can result
from bonding of 2
amino acids
1.0 x 1026 peptides can From: Elliott, WH. Elliott, DC. (1997) Biochemistry
and Molecular Biology. Oxford: Oxford University
be formed from 20 Press. p23
amino acids
Primary structure of insulin
Linear sequence of
amino acids forms
primary structure
Sequence essential for
proper physiological
function
Random coil
- helix
Shape
maintained by
hydrogen bonds
between C=O
and N-H groups
in backbone
R groups
directed
outward from
coil
From: Elliott, WH. Elliott, DC. (1997) Biochemistry and Molecular
Biology. Oxford: Oxford University Press. p28
- pleated sheet
Structure
maintained by
hydrogen bonds
between C=O and
N-H groups in
backbone
R groups directed
above and below
backbone From: Elliott, WH. Elliott, DC. (1997) Biochemistry and Molecular
Biology. Oxford: Oxford University Press. p29
Random coil
Not really random
structure, just non-
repeating
‘Random’ coil has fixed
structure within a given
protein
Commonly called
‘connecting loop region’
From: Elliott, WH. Elliott, DC. (1997) Biochemistry Structure determined
and Molecular Biology. Oxford: Oxford University
Press. p27 by bonding of side
chains (i.e. not
necessarily hydrogen
bonds)
Tertiary protein structure
Covalent bond
between sulfur
atoms on two
cysteine amino
acids
Ions on R
groups form
salt bridges
through ionic
bonds
From: Summerlin, LR. (1981) Chemistry for the Life Sciences. New
York: Random House, p459
Tertiary structure -
hydrophobic forces