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BIOL 171 Lab

Fall 2014
Michelle Smith

Contact Information:
Email: miliefsk@hawaii.edu
Class Web Sites:

• Syllabus
• Schedule
• Assignments
• Handouts
Lab Time & Location
• Section 62109
Monday 2:30-5:15
• Section 62356
Monday 5:30-8:15
Imiloa 106
Instructor’s Office
Location & Hours

Imiloa 136
M 12:45-2:30, W 10:30-11:30
or by apt.
Biology and diversity of:
• Measurements and statistics
• Biological Molecules
• Microscope use and cells
• Enzyme kinetics
• Meiosis and mitosis
• Photosynthesis
• Genetics
• DNA electrophoresis
• Bioinformatics
• Prokaryotes
Cell Phones
Please turn
it off before
arriving to
• Mandatory
• Be on Time!
• Don’t expect labs to end
• Let instructor know if you
anticipate an absence.
Lab Notebook
• 60 pts
• 4 note books lined
• turned in next lab
Figure 1. Frequency histogram of
number of seeds per pod for
koa haole, Leucaena
Date: 9/30/14 leucocephala, seed pods.
Lab 04 Cells
Fig. 1. pseudostratified epithelia

pseudostratified epithelia


Lab Summaries
• 240 pts
• 12 lab summaries (20 pts each)
• Late summaries deduct 3 pts
• More than 1 week late not accepted

• Answer questions
• Purpose, procedures,
results, discussion
• Be organized!
Library Research Paper
• 20 pts
• literature searches, scientific
writing, citation procedures,
and bibliography formatting
• 5 pages minimum
• Late- 5 points deduction up to
one week WCC Library

Fritts, T.H. 1988. The brown tree snake, Boiga
irregularis, a threat to Pacific islands. U.S. Fish Wildl.
Serv., Biol. Rep. 88:23-25.
Pre-Lab Quizzes
• 13 pre-lab quizzes (15 points each) for 150 pts
• Posted @ laulima
• Go to BIOL 171L site
• May get questions from previous lab as well as
current lab
• 10 best scores
• No make-up quizzes
• Don’t be late
• Timed- 20 minutes
Lab Practicals:
• 2 exams
• 100 points each (200 pts)
• No make-up or retake

Form study groups

• Practical Examinations (2) 200 points
• Pre-Lab Quizzes (13) 150 points
• Laboratory Notebook (13) 60 points
• Laboratory Summaries (13) 240 points
• Library Research Report 20 points
TOTAL 670 points
Grade cutoffs:
A = above 90%
B = 80%-89%
C = 70%-79%
D = 60%-69%
F = below 59%

No plus or minus final grades

Adams, B. & Crawley, J. (2013). Van De
Graff's Photographic Atlas for the Biology
Laboratory (7th edition). Morton Pub. ISBN.
161731058-1 (~$40)
• Cheating

• Plagiarism

See student handbook on conduct code or

Lab Safety

Lab Policies
• Sign Waiver
• Read assignment before
attending lab
• Take pre-lab quiz up to
1 hr before lab
Lab Attire
You will be working with some toxic
substances in lab

• Lab coat
• Gloves
• Goggles
• Proper clothing
• No open-toed shoes
Lab Safety

• Food
• Drink
• Smoking
• Cell phone
• Horseplay
• Open-toed shoes
Lab Safety
• Material Safety Data Sheet
(MSDS) for each chemical used is
available to you upon request.
• Identify the location of the fire
extinguisher, first aid kit,
emergency shower, and eyewash
Handling Chemicals

• Treat all chemicals as

though they were
• Do not mouth-pipette
any chemical.
• Do not ingest, inhale,
touch any chemical.
Handling Organisms
• All organisms, living or dead, should be
treated with care and respect.
• Some organisms may be hazardous if
touched or ingested.
• Avoid direct handling when possible.
• Proper handling of microorganisms will
be demonstrated.
Lab Safety
Please Report The Following
to the instructor:

• Injuries
• Broken equipment
• Broken glass or tools
Lab Safety
• Broken glassware,
slides and coverslips
must be disposed of
into the “GLASS
ONLY” boxes

These guidelines will help to protect

you from the harmful materials used in
Lab Safety Equipment
• In case of an emergency,
you should know the
location and proper use
of all the safety
equipment provided in
the laboratory.
• Always immediately
inform the instructor of
any accident.
Fire Extinguishers (3)
Pull out
locking key.

Aim nozzle
at base of
Fire Extinguishers
• Carbon dioxide fire extinguishers are suitable
for many of the potential fire hazards in the
general chemistry laboratories.
• To operate this type of fire extinguisher, aim the
nozzle at the base of the fire, pull out the
locking key and depress the handles together.
• This will release a discharge of carbon dioxide
that will smother the fire.
• Always inform the instructor of the use of a fire
extinguisher so that it can be recharged.
Safety Eyewash

Any chemical
contacting one’s eyes
should be
immediately and
thoroughly rinsed out
using the safety
eyewash found in
every laboratory.
Position head Push handle to start
between spigots. the flow of water!
Safety Eyewash

• Call for assistance to get to the eye

wash station.
• Position the head with eyes open
between the spigots.
• Push the handle to start the flow of
• Flush for at least 15 minutes.
Safety Shower
Any chemical spill on
a person should be
washed off
immediately and
thoroughly using the
safety shower.

Pull the chain to start

the flow of water!
Safety Shower
Any chemical spill on a person
should be washed off immediately and
thoroughly using the safety shower.

• Remove all contaminated clothing and

stand under the shower.
• Pull the chain to start the flow of water.
• Washing should continue long enough to
insure complete removal of the chemical, at
least 15-20 minutes.
Chemical Spills
• Located on lab benches are containers
of sodium bicarbonate to neutralize acid
spills. These work by covering the spill
of acid with a solid.
• Contact your instructor immediately if
there is a chemical spill.
• Your instructor will handle any and all
chemical spills.
Special Caution Handling Glassware

Protect hands.

Lubricate glass NEVER force

surfaces. the glassware!
Special Caution Handling Glassware

• Many laboratory accidents involve cuts or

puncture wounds due to improper
manipulation of glassware.
• Never force a piece of glassware when
constructing experimental apparatus!
• Use proper hand protection and lubricants
such as glycerin or soapy water when
Waste Disposal

Discard waste as needed, during

and at the end of each laboratory period.
Waste Disposal
• Specific
guidelines need
to be observed
for waste
disposal and will
be given by your
• Not Sure?
Waste Basket
Paper Products

Paper products
that do not
chemicals can
be placed in the
waste baskets.

Waste Basket
Solid and Liquid Chemicals
• Solid and liquid
chemicals need to
be placed in
• The exact means
will be given in the
lab procedure or
specified by the
Never dump chemicals down the drain!
Never return unused chemicals to the original
stock bottle!
• Have a positive attitude!
• Work cooperatively with group
• Everyone participates
• Disinfect your work area
• Put equipment away
Most Important
• Keep you eyes open to
your surroundings.
• If your not sure, ASK!