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Organizing Science

Research Papers (1)




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As an island, Taiwan has abundant seafood available for consumption. Of
particular concern is Vibrio paraemolyticus, an epidemic pathogen commonly found in
seafood. This pathogen can cause sitotoxism, a disease with an incidence rate in
Taiwan that is the highest worldwide. Often a result of insufficiently cooked seafood,
sitotoxism is especially prevalent from May to August, when a large amount of seafood is
consumed islandwide.
As the conventional means of identifying Vibrio paraemolyticus, serotyping is
expensive, tedious, and time consuming. Various molecular subtyping methods are also
applied to identify an appropriate means of differentiating Vibrio paraemolyticus isolates
from other serotypes. However, whether molecular subtyping methods provide a more
rapid and sensitive alternative for differentiating among Vibrio paraemolyticus strains is
unknown.
For instance, serotyping requires seven days before supplying reliable
results and, in doing so, is relatively inefficient, thus necessitating the need for a viable
alternative.
The inability to develop a more reliable and efficient means of identifying
Vibrio paraemolyticus will lead to an increasingly higher incidence rate of sitotoxism in
Taiwan.
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Based on the above, we should develop a rapid molecular
identification method using the high specific gene of tdh gene in bacteria
chromosome to detect a major target.
To do so, tdh gene can be detected by polymerase chain
reaction (PCR). Exactly how V. paraemolyticus and antibiotics are related can
then be determined using a drug sensitivity test. Next, using this same test,
whether bacteria are drug resistant or drug sensitive can be determined as well.
Additionally, the Vibrio paraemolyticus epidemic phenomenon in the
surrounding environment can be more thoroughly understood using pulsed field
gel electrophoresis.
As anticipated, the proposed molecular identification method can
detect the presence of Vibrio paraemolyticus in the environment in only a few
hours. Additionally, the pulsed field gel electrophoresis results can provide
further insight into the molecular epidemic phenomenon in the surrounding
environment.
Importantly, the proposed method can provide rapid and accurate
molecular biology results to detect Vibrio paraemolyticus in the environment.
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As is well known, ionizing irradiation causes material
damage. The range/number of radiation effects in solids depends
on both the crystal structure and the type of ionizing irradiation.
Additionally, the energy and dosage of irradiation essentially affect
radiation processes. Ionizing irradiation excites both electronic and
ionic subsystems of crystals. Relaxation processes occur after
excitation, forming defects. Defects impair the solids, extensively
stimulating material damage.
However, the radiation-stimulated ordering effect (RSOE)
is found in various materials. At least two competitive processes
are observed in crystals and semiconductors under ionizing
irradiation 1) generation of radiation defects and 2) radiation-
stimulated annihilation of defects.
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For instance, on a specific stage, the annihilation
of irradiation defects can dominate the generation of defects,
enhancing the structure. This stage is referred to as RSOE.
Therefore, low-dose irradiation could serve as an effective method
for increasing the reliability of semiconductors and the stability of
parameters in the final stage of manufacturing. This fact
substantially increases economical efficiency.
Nevertheless, the RSOE mechanisms in II-VI
semiconductors remain unknown, making it impossible to implement
process applications capable of increasing the efficiency of barrier
structures. Although the application of RSOE could markedly
increase product output in microelectronics, the generalization and
analysis of RSOE remain an obstacle to further development.

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Based on the above, we should investigate the
RSOE in II-VI semiconductors and construct a related
model.
To do so, voltage-current characteristics
(VIC), voltage-capacity characteristics (VCC) and capacity-
modulated spectra for barrier structures can be measured
at various irradiation doses, providing the preliminary
experimental data. Based on that data, the control
parameters can then be derived. Following Hall
experiments to consider bulk effects, the parametric
changes can be analyzed and synthesized.
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As anticipated, low-dose radiation processes can be
clarified in solids, improving the parameters of structures based on II-VI
semiconductors. The proposed work can also elucidate low-dose
radiation processes in II-VI semiconductors. Thus, applying this model
to barrier structure manufacturing can allow us to accurately predict the
irradiation conditions to enhance the parameters of structures based on
II-VI semiconductors. The range of objects in which the RSOE is
observed can be expanded. II-VI and other (Si, III-V) semiconductors
can also be compared in terms of the RSOE mechanisms. Analysis of
RSOE in II-VI reveals the peculiarities of related objects.
In contrast with III-V semiconductors, for which the
properties of pure crystals can be determined by impurities in II-VI
semiconductors, the lattice stoichiometric defects prevail. Naturally,
RSOE can be explained by the reconstruction of defect centers.
Importantly, the proposed model can consider the effect of the
radiation-stimulated diffusion (RSD) of point defects.

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