Microbial Degradation of Fiber in a Consortium of Human Gut Species

Ideas and Questions?

1. Variables 2. Conditions (simulating the Human Gut?) 3. Method and Control/Monitoring

 Bacterias - pH and Temperature: pH 8 in large intestine, and 37 - Triplicate Samples.

- Ruminoccocus Gnavus (Gram +) C body temperature.
- Bacteroide Fragillis (Gram -) - % Concentration of Substrates?
- Bifidobacterium Bifidum (Gram +) - Anaerobic Cabinet.
- Arkemmansia Muciniphila (Gram -) - Feed or Add Substrate 3 times per day,
- Faecalibacterium Prausnitzii (Gram +) - Is necessary to simulate peristalsis? simulating Human Diet?
[Most Common/Prevalence/Higher Concentration]
- Simulate Mix of Substrate at diferent concentration - Analysis and Control 1 time per day
 Fiber (*according to Perspective on the complexity of
like human everyday diet?.
- Corn/Potato Starch dietary fiber srtuctures..)
- Pectin (Apple/Citrus Peel)
- Cellulose - Total Test Time: 5 days
[More complex and abundant in our human diet; aligned with
vegan and freshness tendency]

 Growth Medium?

6. Challenges
4. Analysis 5. More Questions?
- Undestand Fiber structure?
- Lab Analysis ? 16S rRNA? - Costs? Budget?
- Understand and Figure out affinity for
- How to determine the subproducts and final - Scope and Limits of Research
substrate, and complex Fiber degradation
products of fiber degradation?
ways of each bacteria?

- Understand and link the relation between the

bacterias and their metabolic products,
cooperation or competition?