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First lecture
2
Radiation ionizes
the gas. Ions
move toward
http://www.science.uwaterloo.ca/~cchieh/cact/nuctek/int electrodes,
eractdetector.html creating current.
8
Ion chamber continued
• Voltage is high enough that ions
reach the electrodes, produce current.
• Proportional to energy: the more
energy, the more current.
• Generally requires some amplification
of the signal.
• Example of use: pocket dosimeters
http://www.ludlums.com/images/dosimeter.jpg
Proportional Counters 9
• At some hundreds volts, the improvement in sensitivity is more than
collecting all the When voltages applied to electrodes of ionization
chambers increase, the sensitivities increase. Electrons and ions on the
electrode. The currents corresponding to multiples of ions and electrons
produced by radioactivity. To distinct them from simple ionization
chambers, these detectors are called proportional counters.
• In proportional counters, the high voltage applied to the electrodes
created a strong electric field, which accelerate electrons. The electrons,
after having acquired the energy, ionize other molecules. Production of
secondary ion pairs initiates an avalanche of ionization by every primary
electron generated by radiation. Such a process is called gas
multiplication.
• The gas multiplication makes the detection much more sensitive. Yet, the
current is still proportional to the number of primary ion pairs.
• When voltages applied to proportional counters get still higher, sparks
jump (arcs) between the two electrodes along the tracks of ionizing
particles. These detectors are called spark chambers, which give
internal amplification factors up to 1,000,000 times while still giving an
initial signal proportional to the number of primary ion pairs.
10
Proportional counters -+
• Each ionization electron is
-+-+
accelerated by the voltage
so that it ionizes more of -+-+-+-+
the gas.
– The higher the energy of -+-+-+-+
the radiation event, the -+-+-+-+
greater the avalanche, the
higher the current -+-+-+-+-+-+-+-+
– Each ionization event -+-+-+-+-+-+-+-+
detected separately.
.................
.................
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Geiger Mueller counters
http://www.pchemlabs.com/images/eberline-rm20-geiger-counter-a.JPG
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How Geiger counters work
• Voltage is high enough that every radiation event
triggers a complete avalanche of ionized gas
– Does not discriminate among different energy
levels
– Each event is registered
• A quenching agent stops the reaction, resets gas
for next event
• Slow response time (comparatively) but simpler
circuitry.
• Good for simple, sturdy, instruments
• Best for gamma; low efficiency for alpha, beta.
13
More Geiger details
Higher voltage leads to
constant avalanches;
instrument “pegs”.
http://www.fnrf.science.cmu.ac.th/theory/radiation/Radiation%20and
%20Radioactivity_files/image018.gif
17
Liquid Scintillation counters
• Workhorse in biology labs for many years
• Very useful for beta emitters, some alpha
• Modern equipment:
– Computer driven
http://www.gmi-inc.com/Genlab/Wallac
%201414%20LS.jpg
18
Basic principles
• Radioactive sample is mixed with organic
solvents (cocktail)
• Toluene replaced with biodegradable solvents
• Detergents allow up to 5% aqueous samples
• Radiation hits solvent, energy is absorbed by
solvent; Energy passed to one or more fluors
• Fluor emits visible light which is detected
– By fluorescence
– Amplified by photomultiplier, converted to
electrical signal.
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20
Coincidence circuitry
• Photomultipliers very sensitive
– Inside of instrument completely dark
– Tubes give off “thermal electrons”
• Result would be very high background counts
• Coincidence circuitry compares results from
2 photomultipliers
– Event not detected by both: thermal electron
• Ignored
– Event detected by both is affect of beta particle
• Counted.
21
Counts and energy discrimination
• As radiation travels through solvent, it gives
up energy
– The more energy it has, the more fluor molecules
get excited and release photons
– Thus, the higher the energy, the brighter the flash
• The higher the electrical pulse sent from the
PMs
• Instruments can be electronically adjusted
– Discriminators set for different “pulse height”
– Able to count betas from H-3 vs. C-14 vs. P-32
22
Pulse height
23
Summary of capabilities
• Pulse height
– From brightness of flash; the more energetic the
radiation, the brighter the flash.
– Discriminators (“gain”) in the instrument can be set
so you determine what energy you want counted.
• Number of pulses
– Corresponds to how many flashes, that is how
many radiation events (decays): the amount of
radioactivity.
24
Difficulties with LSC
1. Static electricity: causes spurious high counts,
esp. when humidity is low;
1. don’t wipe outside of vials!
2. Chemiluminescence: chemical reactions in
sample, from overhead lights, glass.
1. Suspiciously high counts can be redone; chemi-
induced high counts subside over time.
3. Quench
1. Anything that interferes with counting efficiency.
1. Measured: counts per minute (cpm)
2. Desired: decompositions per minute (dpm)
25
Counting efficiency
• Because samples are usually dispersed in clear
containers, geometry is favorable for energy
transfer in all directions and good light emission
• Not all decay events will get registered,
however, because no system is 100% efficient
• We seek to know the # of decompositions per
minute (dpm) but measure the counts per
minute (cpm).
• Using standards helps determine efficiency.
26
Effect of Quench
27
All about quench
• Chemical quench
– Acids, bases, high salt, any chemical that interferes
with transfer of energy from the solvent to the fluor.
– Result: fewer activated fluor molecules, less intense
flash, interpreted as a lower energy event.
• Color quench
– Colored material absorbs visible light from fluor
– Less intense flash, appears as lower energy event
28
About quench -2
• Self absorption
– If particulate matter not well suspended, energy
not absorbed by fluor, not detected as well. Both
lowering of cpm and forcing into lower energy
range.
29
Counting statistics
• Radioactive decay is a random event
– To be sure results are reliable, a minimum number
of decay events must be recorded.
– Reliability depends on total number of counts!
• Example
– Statistical significance is the same in these two
cases;
• 10 minute count yielding 500 cpm
• 1 minute count yielding 5000 cpm.
– Both have total of 5000 counts
– Instruments have settings for stopping count when
a certain statistical threshold is reached.