Universidad del Zulia

Facultad Experimental de Ciencias

División de Estudios para Graduados
Programa de Educación Continua
Maestría en Biología mención Inmunología Básica
Curso Teórico

CÉLULAS MADRE Y TERAPIA CELULAR

INTRODUCCIÓN AL CURSO DE
CELULAS MADRE Y TERAPIA CELULAR

Héctor Pons
hpons@fmed.luz.edu.ve
0414 362.3287
And how can you mend a broken heart?
How can you stop the rain from falling down?
How can you stop the sun from shining?
What makes the world go round?
How can you mend a this broken man?
How can a loser ever win?
Please help me mend my broken heart and let me live again.
 Related MedlinePlus Pages:
•Heart Diseases - http://www.nlm.nih.gov/medlineplus/he
•Stem Cells - http://www.nlm.nih.gov/medlineplus/stemc
      Date last updated: 13 October 2009

Lab Study Makes Strides in Mending Hearts

Mouse stem cells are being used to help develop a heart muscle patch
URL of this page: http://www.nlm.nih.gov/medlineplus/news/fullstory_90476.html (*this news item will not be available after 01/10/2010)

 
HealthDay

Robert Preidt
Monday, October 12, 2009

Using mouse embryonic stem cells, Duke University bioengineers performed a

M
series of lab experiments that mimicked the way embryonic stem cells develop
into heart muscle.
The researchers used a special mold they created to make a 3-D "patch" made of
ONDAY, Oct. 12 (HealthDay News) -- U.S. researchers have achieved a first step toward growing a living "heart patch" to repair damage from heart
heart muscle cells called cardiomyocytes. The mold had features that enabled the
disease.
researchers to control the direction and orientation of the growing cells.
Using mouse embryonic stem cells, Duke University bioengineers performed a series of lab experiments that mimicked the way embryonic stem cells develop into
heart muscle. The newly created tissue displayed two critical features of heart muscle cells --
The researchers used a special mold they created to make a 3-D "patch" made of heart muscle cells called cardiomyocytes. The mold had features that enabled the
researchers to the
controlability toandcontract
the direction and
orientation of the to cells.
growing conduct electrical impulses, the study authors
The newly created tissue displayed two critical features of heart muscle cells -- the ability to contract and to conduct electrical impulses, the study authors explained
explained
in a Duke University in a Duke University news release
news release.
The results were scheduled to be presented at the Biomedical Engineering Society's annual scientific sessions, held Oct. 7 to 10 in Pittsburgh.
"While we were able to grow heart muscle cells that were able to contract with strength and carry electric impulses quickly, there are many other factors that need to be
considered," Nenad Bursac, an assistant professor at Duke's Pratt School of Engineering, said in the news release.
The rate of heart muscle cell development is just one area that requires further research.
"Human cardiomyocytes tend to grow a lot slower than those of mice," Bursac said. "Since it takes nine months for the human heart to complete development, we need to find
a way to get the cells to grow faster while maintaining the same essential properties of native cells.“
SOURCE: Duke University, news release, Oct. 10, 2009
HealthDay
Copyright (c) 2009 ScoutNews, LLC. All rights reserved.
1908. El histólogo Ruso Alexander Maksimov (1874-1928), en un congreso
de la sociedad de hematología, en Berlín, dijo que debía existir célula madre
Hemopoyeticas.
The Lymphocyte as a stem cell common to different blood elements in embry
onic development and during the post-fetal life of mammals
, translated from German, originally in: Folia Haematologica 8.1909, 125-134.

1960. Joseph Altman y Gopal Das, Neurogenesis del adulto, Trabajo ignorado
1963
1968, Trasplante de medula ósea
1978, Se descubren las HSC en sangre del cordón
1981, Martin Evans, Matthew Kaufman y Gail Martin, aislaron CM embrionarias, de la
Masa celular interior (Martin usó el termino ESC por primera vez) RATÓN
1992, se cultivaron las CM Neurales
1997, Leucemia derivada de HSC, primera evidencia de origen del cáncer en Cel. Madre
1998, James Thompson et al. ESC lines HUMANAS
2007. iPSC Humanas
2009. phiPSC
Reprinted from RADIATION RESEARCH
Volume 13, No. 1, July 1960
Copyright © by Academic Press Inc. Printled
in U.S.A.
RADIATION RESEARCH 13, 115-125 (1960)
The Radiation Sensitivity of Normal Mouse
Bone Marrow Cells,
Determined by Quantitative Marrow
Transplantation
into Irradiated Mice1
E. A. McCULLOCH AND J. E. TILL
Department of Medical Biophysics, University
of Toronto, and the Divisions of Biological
Research
and Physics of the Ontario Cancer Institute,
Toronto, Ontario
Reprinted from RADIATION RESEARCH,
Volume 14, No. 2, February 1961
Copyright @ 1961 by Academic Press Inc.
Printed in U.S.A.
RADIATION RESEARCH 14, 213-222 (1961)
A Direct Measurement of the Radiation
Sensitivity of
Normal Mouse Bone Marrow Cells1
J. E. TILL AND E. A. McCULLOCH
Department of Medical Biophysics, University
of Toronto, and the Divisions of
Biological Research and Physics of the Ontario
Cancer Institute,
Toronto, Ontario

(Reprinted from Nature, Vol. 197, No. 4866, pp. 452-454,

February 2, 1963)
CYTOLOGICAL DEMONSTRATION OF THE CLONAL NATURE OF SPLEEN COLONIES DERIVED
FROM TRANSPLANTED MOUSE MARROW CELLS
By DR. A. J. BECKER, E. A. McCULLOCH and J. E. TILL
Departrnent of Medical Biophysics, University of Toronto and Ontario Cancer Institute, Toronto
1908. El histólogo Ruso Alexander Maksimov (1874-1928), en un congreso
de la sociedad de hematología, en Berlín, dijo que debía existir célula madre
Hemopoyeticas.
The Lymphocyte as a stem cell common to different blood elements in embry
onic development and during the post-fetal life of mammals
, translated from German, originally in: Folia Haematologica 8.1909, 125-134.

1960. Joseph Altman y Gopal Das, Neurogenesis del adulto, Trabajo ignorado
1963
1968, Trasplante de medula ósea
1978, Se descubren las HSC en sangre del cordón
1981, Martin Evans, Matthew Kaufman y Gail Martin, aislaron CM embrionarias, de la
Masa celular interior (Martin usó el termino ESC por primera vez) RATÓN
1992, se cultivaron las CM Neurales
1997, Leucemia derivada de HSC, primera evidencia de origen del cáncer en Cel. Madre
1998, James Thompson et al. ESC lines HUMANAS
2007. iPSC Humanas
2009. phiPSC
Generation of Pluripotent Stem Cells from Adult Mouse Liver and Stomach Cells
Takashi Aoi,1,2 Kojiro Yae,1 Masato Nakagawa,1 Tomoko Ichisaka,1,3 Keisuke Okita,1
Kazutoshi Takahashi,1 Tsutomu Chiba,2 Shinya Yamanaka1,3,4,5*
www.sciencemag.org SCIENCE VOL 321 1 AUGUST 2008
1908. El histólogo Ruso Alexander Maksimov (1874-1928), en un congreso
de la sociedad de hematología, en Berlín, dijo que debía existir célula madre
Hemopoyeticas.
The Lymphocyte as a stem cell common to different blood elements in embry
onic development and during the post-fetal life of mammals
, translated from German, originally in: Folia Haematologica 8.1909, 125-134.

1960. Joseph Altman y Gopal Das, Neurogenesis del adulto, Trabajo ignorado
1963
1968, Trasplante de medula ósea
1978, Se descubren las HSC en sangre del cordón
1981, Martin Evans, Matthew Kaufman y Gail Martin, aislaron CM embrionarias, de la
Masa celular interior (Martin usó el termino ESC por primera vez) RATÓN
1992, se cultivaron las CM Neurales
1997, Leucemia derivada de HSC, primera evidencia de origen del cáncer en Cel. Madre
1998, James Thompson et al. ESC lines HUMANAS
2007. iPSC Humanas
2009. phiPSC
 Aspectos que deben ser claramente entendidos en este curso:

Qué son las Células Madre, que características posee y por que son importantes?

Que diferencias conceptuales y funcionales existen entre:

Células Madre Embrionarias, Células Madre somáticas o adultas y Células Madre
inducidas a Pluripotentes iPSC. piPSC (inducida por proteinas)

Tener claros los conceptos de Auto Renovación, AR sostenida (long term),

Proliferación y Diferenciación. Plasticidad vs diferenciación y las Potencias, desde la
Omnipotencia hasta la impotencia.

El concierto de señales que afecta a la célula: Internas (genes), externas (proteínas y

otras células o microambiente)

Como se identifica una célula como Célula Madre?

Quimeras, Teratomas, Crecimiento sin diferenciación in vitro, ensayo clonogénico,
factores de transcripción como Nanog y Oct4, Marcadores en general…
Como se “dirige” la diferenciación
De Células Madre embrionarias?

Récipes y protocolos
Para que sirve la Terapia Celular basada en Células Madre?
Geron Receives FDA Clearance to Begin World's First Human Clinical Trial of Embryon ic Stem Cell-Based Therapy News releases Geron .mht - Shortcu t.ln k
Patient Application for the Institute of Cellular Medicine.pdf - Shortcut.lnk
http://stemcells.nih.gov/info/basics/basics7.asp
http://stemcells.nih.gov/info/basics/
http://www.nature.com/stemcells/index.html
http://www.stembook.org/

http://www.nlm.nih.gov/medlineplus/stemcells.html