Science Project

Recommendations (7)


http://www.chineseowl.idv.tw


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During our most recent weekly group meeting,
participants expressed concern over the increasing incidence of
food poisoning in humans from aquatic foods.
Widely distributed in aquatic environments, bacteria in the genus
Vibrio are considered autochthonous bacteria in marine and
estuarine waters. Capable of causing infection in humans, some
Vibrio species have been isolated from a variety of intestinal and
extraintestinal sites. Specifically, this bacteria can cause
gastroenteritis in humans. PCR method is the conventional
means of detecting the target with the tdh gene or trh gene, in
which the specificity in the chromosome of V. parahaemolyticus
gene of chitinase can be used as the detection marker.
However, this method is inefficient in terms of detecting
the target with the tdh gene or trh gene. (NOTE : Add 2-4
sentences that describe characteristics of the problem or
statistics that reflect its severity)
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For instance, PCR method
requires seven days to authenticate the results.
However, the PCR method can be used as long as
the effect can be authenticated. The molecular
biology method is an effective means of
authenticating the results efficiently.
The inability to detect the target with the
tdh gene or trh gene more efficiently will lead to a
higher incidence of food poisoning in humans from
aquatic foods, with an especially high incidence
during the spring season.
(:)
Therefore, we recommend developing a PCR-based
molecular biology method to identify a particular gene of Vibrio
parahaemolyticus rapidly. To do so, this gene in a
chromosome can be extracted from bacteria. Amplification of this gene
can be achieved by using the PCR method. PCR-based assays can then
be made by preparing all of the genomic DNAs of the strains in Tris-
EDTA buffer (TE; pH 8.0)according to previous literature. Next, the purity
and the amount of DNA in each preparation can be estimated
colorimetrically, with the DNAs subsequently stored at 4C until further
use. Additionally, whether germs can be found in seafood can be
determined using the PCR method. As anticipated, the
proposed PCR-based molecular biology method can rapidly identify the
presence of marine bacteria in the early stages. In addition
to providing quick results with a high degree of sensitivity, the proposed
method can yield rapid and accurate results to detect Vibrio
parahaemolyticus in the environment. (NOTE : Add 2-4 more sentences
that describe more thoroughly how the proposed method contributes to a
particular field or sector)
(:)
At a recent meeting, members
discussed how to determine the period and location of
gene expression. Gene expression
requires that many proteins interact with a regulated
element. However, modulation of gene expression has not
been thoroughly investigated, making it impossible to
determine the period and location of gene expression.
However, expression of the same gene in a
diverse cell can be regulated based on different
expressions. (NOTE : Add 2-4 sentences that describe
characteristics of the problem or statistics that reflect its
severity) and, thus, cannot be
expressed 100%. The inability to
thoroughly understand the mechanism of distinct gene
expression makes it impossible to understand how this
unique mechanism affects embryo development.
(:)
Therefore, we recommend elucidating the
mechanism of gene expression in a cell owing to the
importance of acquiring genes in a cell for embryo
development. To do so, the regulatory role
of a gene in the upstream as promoter and enhancer can be
analyzed by adopting molecular cloning methods. Next, the
identified gene can be linked with a regulating gene to clarify
how gene expression sites are regulated. As
anticipated, analysis results can clarify the mechanism of
gene expression in cells and regulation of tissue-specificity
genes. Results of this study can provide a
valuable reference for efforts to develop a therapeutic
method of gene expression for patients with genetic defects.
(NOTE : Add 2-4 more sentences that describe more
thoroughly how these results contribute to a particular field or
sector)
Further details can be found at
http://www.chineseowl.idv.tw