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SETUP
F1
F2
F3
Pancreatin
- lipase, protease, amylase: catalyze hydrolysis of fats into glycerol and fatty acid
Bile Salt
- emulsifies fats - helps in eliminating toxins from the body - fats mixed with bile salts can be broken down by pancreatic lipase into fatty acids monoglycerides
SETUP
Measure intial pH of each solution.
SETUP
Incubate in a 37C water bath for an hour. Repeat pH measurement of each solution after 20, 40, and 60 minutes incubation.
7 6.2 6.71
WHAT HAPPENED?
as the incubation time increased, the pH of solution of cream with pancreatin decreased. as pancreatic enzymes and bile breaks the ester bonds of triglycerides in the cream, pH continues to decrease because the work of the enzymes is starting to effect.
the enzymes are starting to emulsify fats from the cream. That explains the slight decrease in pH after 60 minutes of incubation.
CHEMICAL DIGESTION
Process by which food products are chemically processed by the body starts in the mouth and then in the stomach Salivary amylase Hydrochloric acid, gastric juices enzymes are used to break down food Pancreatic enzymes bile
DIGESTIVE ENZYMES:
Lipase
released by the pancreas contains enzymes that break down carbohydrates, fat and protein
Bile
released by the liver dissolves fat so that the pancreatic juices (lipase) can digest it properly
CHEMICAL DIGESTION
factors like pH may influence enzyme functions during chemical digestion enzymes of digestion are more efficient in a neutral and slightly basic environment than in acidic one. any changes from the particular pH generally decrease catalytic activity. fats are the most difficult to digest since enzymes require a high pH for activation of pancreatic enzymes when there is no food to be broken down in the stomach, pancreatic enzymes and bile are not present pancreatic enzymes and bile salts also act as alkalinity regulator in the stomach.
CHEMICAL DIGESTION
addition of bile salts hastens fat digestion and toxin elimination from the body. Thus, samples containing bile salts should have exhibited a decreasing trend in pH.
RATE OF DIGESTION VS PH